Sragvi Verma ^1,2 , Diya Nanthakumarvani ^1,2 , Leesha Haarshiny Perumal ^1,2 , Atoshi Abirami RajKumar ^1,2 , Cheryl Kai Ning Kang^1,2 , Shafeeqa Abul-Hasan^1,2 and Maurice HT Ling^2,3,4 *

¹ Department of Applied Sciences, Northumbria University, United Kingdom
² Management Development Institute of Singapore, Singapore
³ Newcastle Australia Institute of Higher Education, University of Newcastle, Australia
⁴ HOHY PTE LTD, Singapore

*Corresponding Author: Maurice HT Ling, Management Development Institute of Singapore, Singapore.

Received: December 29, 2025; Published: January 31, 2026

Abstract

Streptococcus thermophilus is a lactic acid bacterium, which is used in yogurt production, and known for its ability to produce folate, exopolysaccharides; thus, making it a highly valuable organism for food biotechnology and probiotic applications. Mathematical kinetic models, which extend beyond steady-state predictions in genome-scale models, are useful tools for directing metabolic engineering efforts. Although a genome-scale models of S. thermophilus is available, a comprehensive whole-cell kinetic model is lacking. In this study, we describe a simulatable kinetic model of S. thermophilus STH_CIRM_65, constructed in an ab initio fashion by locating enzymes from the genome sequence and mapping them to corresponding reactions in KEGG. The resulting model, stheVS26, encompasses 322 metabolites, 400 enzymes along with their transcription and translation processes, and 336 enzyme reactions. This model provides a foundational platform for the simulation and prediction of cellular behaviour, allowing for informed design decisions in metabolic engineering.

Keywords: Whole-cell Model; Kinetic Model; Differential Equations; AdvanceSyn Toolkit; Yoghurt; Exopolysaccharides

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Citation

Citation: Maurice HT Ling., et al. “Ab Initio Whole Cell Kinetic Model of Streptococcus thermophilus STH_CIRM_65 (stheVS26)". Acta Scientific Nutritional Health 10.2 (2026): 43-47.

Copyright

Copyright: © 2026 Maurice HT Ling., et al.This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.