Acta Scientific Nutritional Health (ASNH)(ISSN: 2582-1423)

Research Article Volume 4 Issue 4

Microbiological Assessment of Selected Groundnut Based Snack “Kulikuli” and “Donkwa” in Ogbomoso And Ilorin, Nigeria

Adeoye AO1, Adeyinka OO2, Ajala AS1* and Ojo MA3

1Department of Food Science and Engineering, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria
2Anatomy Department, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria
3Department of Food Science and Engineering, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria

*Corresponding Author: Ajala AS, Department of Food Science and Engineering, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria.

Received: February 27, 2020; Published: March 14, 2020

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  In this study, the microbial quality assessments of two peanut based snacks were analyzed. Samples were obtained from ‘Orita Naira’ market, Ogbomosho, Oyo State and ‘Oja Titun’ Ilorin, Kwara State, Nigeria. The controls were prepared under hygienic condition in the Food processing laboratory, Ladoke Akintola University of Technology, Ogbomoso. These samples were analyzed to investigate their safety. Thus, microbial colony estimation and characterization were carried out; these include microbial load, frequency of occurrence and antimicrobial sensitivity test. All the samples had bacterial and fungal contamination at varying degree; microbial load in terms of percentage frequency of occurrence of bacteria was between 5 - 30%, the percentage frequency of occurrence for fungi was 5 - 16%. For bacteria, the highest percentage microbial count was Salmonella spp (30%) while the least was E. coli (5%), while for fungi, the highest and lowest percentage microbial count were 16 and 5% respectively. Total Viable Count ranged from 1.3 ± 0.10 x 103 to 4.10 ± 0.10 x 103 (cfu/ml), coliform ranged between 0.00-5.40 ± 0.40 cfu/ml, while mould count ranged from 1.00 ± 0.00 - 3.87 ± 0.31. Sensitivity test showed that bacteria inhibition zone of 32 mm and 25 mm in Kulikuli and Donkwa respectively, while fungal sensitivity test indicated 26 mm, 32 mm in kulikuli and donkwa respectively. With 30% salmonella spp found in kulikuli and 10% E. coli found in donkwa, it shows poor hygiene in processing and handling.

Keywords: Isolation; Hygiene; Contamination; Processing; Organism

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References

  1. Ezekiel CN., et al. “Microbiology Quality and Proximate Composition of Peanut cake (kulikuli) in Nigeria Markets”. Academic Arena 3.4 (2011): 103-111. 
  2. Adebesin AA., et al. “Microbiological quality of some groundnut products hawked in Bauchi, a Nigerian City”. The Journal of Food Technology in Africa 6.2 (2001): 53-55.
  3. Aletor O and Ojelabi A. “Comparative evaluation of the nutritive and functional attributes of some traditional Nigerian snacks and oil seed cakes”. Pakistan Journal of Nutrition 6.1 (2007): 99-103.
  4. Oladimeji GR and Kolapo AL. “Evaluation of proximate changes and microbiology of stored defatted residues of some selected Nigerian oil seeds”. African Journal of Agricultural Research 3.2 (2008): 126-129.
  5. Talaro KP and Talaro A. Foundations in Microbiology. 4th Ed. The McGraw-Hill Companies, NY. (2002).
  6. Baah-Tuahene S. “Assessment and Safety Improvement of Peanut oil based by-product from peanut oil processin: Kulikuli and Khebab Powder”. MPhil Thesis, University of Ghana (2015).
  7. Bakhiet AO., et al. “Food safety knowledge and practices of street food-vendors in Atbara city (Naher Elneel state, Sudan)”. African Journal of Biotechnology 8.24 (2009): 6967-6971. 
  8. Adeoye AO., et al. “Safety assessment of fermented African locust bean seed (Parkia biglobosa) in Ogbomosa market, Oyo Sate Nigeria”. International Journal of Research in Engineering and Technology 6.1 (2018): 33-40.
  9. Ojo KA., et al. “Safety assessment of Donkua- An Indigenous Maize- peanut Street- vended Snack”. Journal of Food and Agriculture, Nutrition 12.3 (2017): 6-8. 
  10. AOAC. Official Methods of Analysis. 15th Ed. Association of Official Analytical Chemists. Washington DC, USA (1995).
  11. Samson RA., et al. “Methods for the detection and isolation of food-borne fungi”. In Samson RA, ES Hoekstra, JS Frisvad, O Filtenborg (Eds.), Introduction to Food-borne Fungi. 4th Ed. Central Bureauvoor Schimmel cultures, The Netherlands (1995): 235-242.
  12. International Commission on Microbiological Specification for Food (ICMSF 2002) Microorganisms in food. Microbiological testing in food safety management. Kluwer Academic/Plenum Publishers, New York, USA (2002).
  13. Frazier WC and Westhoff DC. Food Microbiology, 23rd Edition, Tata McCgraw Hill Publishing Co, Ltd (2005).
  14. Brown AE. “Benson's Microbiological Application”. 9th Ed. McGraw-Hill, NY. (2005): 249-279.
  15. FAO. Assuring food safety and quality - Guideline for strengthening national food control system. FAO food and Nutrition paper number 76 (2003).
  16. Adegoke GO. “Understanding Food Microbiology”. 2nd Ed. Alleluia, Ibadan (2004): 111-126.
  17. Ezekiel CN., et al. “Zearalenone Production by naturally occurring Fusarium species on maize, wheat and soybeans from Nigeria”. Journal of Biological Environmental Science 2.6 (2008): 77-82.
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Citation

Citation: Ajala AS.,et al “Microbiological Assessment of Selected Groundnut Based Snack “Kulikuli” and “Donkwa” in Ogbomoso And Ilorin, Nigeria". Acta Scientific Nutritional Health 4.4 (2020): 67-71.




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