Acta Scientific Women's Health (ASWH)(ISSN: 2582-3205)

Review Article Volume 2 Issue 10

Follicular Fluid Aspiration and Oocyte Retrieval Techniques, Several Flushing, Difficulty Situations Damage Risk to the Cumulus Complex and Complications

Mohamed Zarqaoui1, Mustafa Zakaria2*, Noureddine Louanjli3, Nisrine En-Naciri4, Mohamed Ennaji5, Malak Jamil6, Amal Kabit7, Saadani Brahim8 and Boutiche Romaissa9

1Strasbourg University Medicine Pole, Endoscopic Surgery Obstetrics and Gynecology Strasbourg, Alsace-Champagne-Ardenne, Specialist, Gynaecologist Obstetrician, General Medical Coordinator of IRIFIV Fertility Center, Casablanca, Morocco
2Ain Shams University, Faculty of Medicine, MD General Practitioner, RB MSc of Reproductive Biology, and Assisted Reproductive Technology, Consultant at IRIFIV Fertility Center, Casablanca, Morocco
3Strasbourg University Medicine, Department of Clinical Biology, Strasbourg, Alsace-Champagne-Ardenne, FR |RB Reproductive Biology, Head of LABOMAC Laboratory, of Clinical Analysis and Assisted Reproductive Technology IRIFIV Center, AFC Center, Casablanca, Morocco
4Ibn Zohr University Higher School of Technology Agadir, Biology, BE Senior Clinical Embryologist, Laboratory IVF Agadir, Souss-Massa, Morocco
5University Hassan II Aïn Chock, Faculty of Sciences and Techniques, BE Bachelor's Degree Biomedical Technologies Senior Clinical Embryologist at IRIFIV Fertility Center, Morocco
6Hassan II Ain Chock University, PhD Student, Laboratory of Molecular Genetic Physiopathology and Biotechnology, Department of Biology, Faculty of Sciences, BE Senior Clinical Embryologist at IRIFIV Fertility Center, Casablanca, Morocco
7University Hassan II Aïn Chock, Faculty of Sciences and Techniques, BE Master’s Degree Applied Biology (Pharmaceutical Quality Control) Senior Clinical Embryologist at IRIFIV Fertility Center, Casablanca, Morocco
8Mohammed V University, Faculty of Medicine and Pharmacy, Rabat| Formerly Director Obstetrics and Gynaecology Department at Moulay Youssef Hospital, Head Medical MD General Medical Coordinator of IRIFIV Fertility Center, Casablanca, Morocco
9Universite des Sciences et de la Technologie Houari Boumediene, Mcs Molecular Pathology and Biotechnology, BE Clinical Embryologist, Laboratory IVF Algeria, Rotaby Fertility Center, Algeria

*Corresponding Author: Mustafa Zakaria, Ain Shams University, Faculty of Medicine, MD General Practitioner, RB MSc of Reproductive Biology, and Assisted Reproductive Technology, Consultant at IRIFIV Fertility Center, Casablanca, Morocco.

Received: August 28, 2020; Published: September 28, 2020

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Abstract

  In Assisted Reproductive Technology (ART), oocyte retrieval is a vital step. It was initially performed through laparoscopy, which was complex and difficult and of low efficiency. Ultrasound-guided transvaginal oocyte retrieval was safer and more effective; it is presently the standard operation for in vitro fertilization (IVF) treatment. Theoretically, oocyte retention is achievable after the initial aspirate due to abnormal development of the follicle or oocyte and human technical factors, and such retention could be overcome by recurrent follicular flushing. Follicular flushing is considered to maximize the number of oocytes retrieved and thereafter to improve the rate of IVF pregnancy. There are a number of factors that can affect egg collection and/or egg damage. These involve variables like pump vacuum flow, velocity, needle bore size and length, follicle pressure and size and collection techniques. Cook Medical Technology, Brisbane, has developed appropriate equipment to study the factors influencing the success of egg collection and the cause of egg trauma.

Keywords: Vacuum Profiles in Aspiration System; Damage to the Cumulus; Clinical Aspects of Oocyte Retrieval; Preparation the Success of Oocyte Retrieval; Retrieval Techniques; Follicular Flushing; Difficulties; Complications

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References

  1. Edwards RG. “Maturation In Vitro of Human Ovarian Oocytes”. The Lancet7419 (1965): 926-929.
  2. Steptoe PC and Edwards RG. “Laparoscopic recovering of preovulatory human oocytes after priming of ovaries with gonadotrophins”. Lancet (1970): 683-689.
  3. Lopata A., et al. “Collection of human oocytes at laparoscopy and laparotomy”. Fertility and Sterility 25 (1974): 1030.
  4. Renou P., et al. “The collection of human oocytes for in vitro fertilization. I. An instrument for maximising oocyte recovering rate”. Fertility and Sterility 35 (1981): 409-412.
  5. Feichtinger W and Kemeter P. “Transvaginal sector scansonography for needle guided transvaginal follicle aspiration and other applications in gynecologic routine and research”. Fertility and Sterility 45 (1986): 722-725.
  6. Cohen J., et al. “Follicular aspiration using a syringe suction system may damage zona pellucida”. Journal of In Vitro Fertilization and Embryo Transfer 4 (1986): 224-226.
  7. Horne R., et al. “Aspiration of oocytes for In vitro fertilization”. Human Reproduction Update 2 (1996): 77-85.
  8. Soussis I., et al. “Follicular fluid levels of midazolam, fentanyl and alfentanyl during transvaginal oocyte retrieval”. Fertility and Sterility 64 (1995): 1003.
  9. Coetsier T., et al. “Propofol anaesthesia for ultrasound guided oocyte retrieval: accumulation of the anaesthetic agent in follicular fluid”. Human Reproduction 7 (1992): 1422.
  10. Waterstone JJ and Parsons JH. “A prospective study to investigate the value of flushing follicles during transvaginal ultrasound‐directed follicle aspiration”. Fertility and Sterility1 (1992): 221‐223.
  11. Tan SL., et al. “A prospective randomized study comparing aspiration only with aspiration and flushing for transvaginal ultrasound–directed oocyte recovery”. Fertility and Sterility 58 (1992): 356-360.
  12. El Hussein E., et al. “A prospective study comparing the outcome of oocytes retrieved in the aspirate with those retrieved in the flush during transvaginal ultrasound directed oocyte recovery for In vitro fertilization”. British Journal of Obstetrics and Gynaecology 99 (1992): 841.
  13. Biljan MM., et al. “Prospective randomized trial of the effect of two flushing media on oocyte collection and fertilization rates after in vitro fertilization”. Fertility and Sterility 68 (1997): 1132-1134.
  14. Bennett S.J., et al. “Complications of transvaginal ultrasound-directed follicle aspiration: A review of 2670 consecutive procedures”. The Journal of Assisted Reproduction and Genetics 10 (1993): 72.
  15. Ho JR and Paulson RJ. “Modified natural cycle in In vitro fertilization”. Fertility and Sterility4 (2017): 572-576.
  16. Simoni MK., et al. “Women's career priority is associated with attitudes towards family planning and ethical acceptance of reproductive technologies”. Human Reproduction 10 (2017): 2069-2075.
  17. Chambers GM., et al. “Assisted reproductive technology in Australia and New Zealand: cumulative live birth rates as measures of success”. Medical Journal of Australia 3 (2017): 114-118.
  18. Haas J., et al. “Coadministration of GnRH-agonist and hCG for final oocyte maturation(double trigger) in patients with low number of oocytes retrieved per number of preovulatory follicles--a preliminary report”. Journal of Ovarian Research 7 (2014): 77.
  19. Awonuga A., et al. “A prospective randomized study comparing needles of different diameters for transvaginal ultrasound-directed follicle aspiration”. Fertility and Sterility1 (1996): 109-113.
  20. Stanger JD and Yovich JL. “Follicle recruitment determines IVF productivity rate via the number of embryos frozen and subsequent transfers”. Reproduction Biomedicine Online3 (2013): 286-296.
  21. Nakamura M., et al. “Analyzing the risk factors for a diminished oocyte retrieval rate under controlled ovarian stimulation”. Reproductive Medicine and Biology 16.1 (2017): 40-44.
  22. Palmsten K., et al.In vitro fertilization, interpregnancy interval, and risk of adverse perinatal outcomes”. Fertility and Sterility5 (2018): 840-848.
  23. James E and Jenkins TG. “Epigenetics, infertility, and cancer: future directions”. Fertility and Sterility1 (2018): 27-32.
  24. Calhaz-Jorge C., et al. “Assisted reproductive technology in Europe, 2013: results generated from European registers by ESHRE”. Human Reproduction 10 (2017): 1957-1973.
  25. Cai Q., et al. “Does the number of oocytes retrieved influence pregnancy after fresh embryo transfer?” PLOS ONE.2 (2013): e56189.
  26. Huo X., et al. “Bisphenol-A and Female Infertility: A Possible Role of Gene-Environment Interactions”. International Journal of Environmental Research and Public Health 9 (2015):11101-11116.
  27. Ji J., et al. “The optimum number of oocytes in IVF treatment: an analysis of 2455 cycles in China”. Human Reproduction 10 (2013): 2728-2734.
  28. Magnusson A., et al. “The number of oocytes retrieved during IVF: a balance between efficacy and safety”. Human Reproduction 1 (2018): 58-64.
  29. Steward RG., et al. “Oocyte number as a predictor for ovarian hyperstimulation syndrome and live birth: an analysis of 256,381 In vitro fertilization cycles”. Fertility and Sterility4 (2014): 967-973.
  30. Pelinck MJ., et al. “Embryo quality and impact of specific embryo characteristics on ongoing implantation in unselected embryos derived from modified natural cycle In vitro fertilization”. Fertility and Sterility2 (2010): 527-534.
  31. Artini PG., et al. “Cumulus cells surrounding oocytes with high developmental competence exhibit down-regulation of phosphoinositol 1,3 kinase/protein kinase B(PI3K/AKT) signalling genes involved in proliferation and survival”. Human Reproduction 12 (2017): 2474-2484.
  32. Ko DS., et al. “Pregnancy and fertilization potential of immature oocytes retrieved in intracytoplasmic sperm injection cycles”. Clinical and Experimental Reproductive Medicine 3 (2015):118-125.
  33. Rienzi L., et al. “The oocyte”. Human Reproduction S1 (2012): i2-i21.
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Citation

Citation: Mustafa Zakaria., et al. “Follicular Fluid Aspiration and Oocyte Retrieval Techniques, Several Flushing, Difficulty Situations Damage Risk to the Cumulus Complex and Complications". Acta Scientific Women's Health 2.10 (2020): 24-33.




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