Shadia AM Lazim¹*, Dirk Geysen², Abdelrahim Idris Abdelrahim¹, Adil MA Salman¹ and Ammar Abdalla Hamid³

¹College of Veterinary Medicine, University of Bahri, Khartoum, Sudan
²Institute of Tropical Medicine, Antwerp, Belgium
³Sudan University for Science and Technology, College of Veterinary Medicine, Sudan

*Corresponding Author: Shadia AM Lazim, College of Veterinary Medicine, University of Bahri, Khartoum, Sudan.

Received: October 15, 2025; Published: November 10, 2025

Abstract

Trypanosoma vivax was isolated from cattle with natural infection in Raga city South of Sudan. It is denoted as DRSS06 (Deleba Raga South Sudan 2006). It was then used in the present study. The parasite was identified in the laboratory of Professor Ahmed Ali Ismail at College of Veterinary Medicine /Sudan University of Science and Technology based on its morphological character. A single polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) assay was used to confirm the T. vivax species.

The comparative characterization of T. vivax DRSS06 from Southern Sudan and T. vivax IRALD700 from Nigeria was done by using of Short oligodeoxyribonucleosite primers (10-mers) of arbitrary sequence in polymerase chain reaction (PCR) to generate genomic fingerprints (RAPD).

There was a similarity in the pathogenicity between the two strains. They had caused severe disease in goats and sheep with clinical signs as anaemia, loss of weight, poor condition, recumbency which culminating in death. However, there was no genetic similarity between the two strains.

Keywords: Characterization; PCR–RFLP; RAPD; Trypanosoma vivax

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Citation

Citation: Shadia AM Lazim., et al. “Comparison between Trypanosoma vivax Strains Isolated from Central and Western of Africa Following RAPD Assay".Acta Scientific Nutritional Health 9.12 (2025): 02-06.

Copyright

Copyright: © 2025 Shadia AM Lazim., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.