Acta Scientific Nutritional Health (ASNH)(ISSN: 2582-1423)

Research Article Volume 9 Issue 4

Identification of Stress Fingerprints Induced by Acrylamide, a Toxic Contaminant in Starchy Food Products, Using Bacterial Bioluminescence

Maryam Ganjavi1,3* and Yangming Martin Lo2,3

1Nutritional Sciences Program, Department of Public and Allied health, School of Community Health & Policy, Morgan State University, Baltimore, MD, USA
2Institute for Advanced Study, Shenzhen University, Shenzhen, China
3Department of Nutrition and Food Science, College of Agriculture and Natural Resources, University of Maryland, College Park, MD, USA

*Corresponding Author: Maryam Ganjavi, Nutritional Sciences Program, Department of Public and Allied health, School of Community Health & Policy, Morgan State University, Baltimore and Department of Nutrition and Food Science, College of Agriculture and Natural Resources, University of Maryland, College Park, MD, USA.

Received: February 18, 2025; Published: March 04, 2025

Abstract

The presence of acrylamide in food is recognized as a significant concern to human health based on its ability to induce cancer and heritable mutations in laboratory animals. This study aimed to characterize the cellular-level damage of acrylamide by bioluminescence stress fingerprinting. Five genetically engineered strains containing selected stress-responsive E. coli promoters fused to the lux CDABE reporter were employed. Results showed that Luminous E. coli DPD2222, having DNA damage responsive promoter, recA, yielded the highest response, followed by luminous E. coli DPD2234, which contained protein damage responsive promoter, grpE. Quantitative and fingerprint assessment of acrylamide damage could be achieved by optimizing bioluminescence cells constructed with different stress-responsive reporter plasmids.

Keywords: Acrylamide; Luminous Bacteria; Biosensor; E. Coli; Stress Damage

References

  1. Gülcan Ü., et al. “Impact of inert and inhibitor baking atmosphere on HMF and acrylamide formation in bread”. Food Chemistry 332 (2020): 127434.
  2. Cheng L., et al. “Investigation of variations in the acrylamide and Nε‐ (carboxymethyl) lysine contents in cookies during baking”. Journal of Food Science5 (2014): T1030-T1038.
  3. Zhao M., et al. “Evaluation of protective effect of freeze‐dried strawberry, grape, and blueberry powder on acrylamide toxicity in mice”. Journal of Food Science4 (2015): H869-H874.
  4. Sansano M., et al. “Effect of pretreatments and air‐frying, a novel technology, on acrylamide generation in fried potatoes”. Journal of Food Science5 (2015): T1120-T1128.
  5. Mucci LA and Wilson KM. “Acrylamide intake through diet and human cancer risk”. Journal of Agricultural and Food Chemistry15 (2008): 6013-6019.
  6. Singh P., et al. “Determination of acrylamide concentration in processed food products using normal phase highperformance liquid chromatography (HPLC)”. African Journal of Biotechnology47 (2010): 8085-8091.
  7. Geng Z., et al. “Determination of acrylamide in starch-based foods by HPLC with pre-column ultraviolet derivatization”. Journal of Chromatographic Science10 (2011): 818-824.
  8. Longhua X., et al. “Determination of trace acrylamide in potato chip and bread crust based on SPE and HPLC”. Chromatographia 75 (2012): 269-274.
  9. Sun S-y., et al. “A facile detection of acrylamide in starchy food by using a solid extraction-GC strategy”. Food Control2 (2012): 220-222.
  10. Weijun Y. “Direct determination of acrylamide in food by gas chromatography with nitrogen chemiluminescence detection”. Journal of Separation Science13 (2015): 2272-2277.
  11. Saraji M and Javadian S. “Single-drop microextraction combined with gas chromatography-electron capture detection for the determination of acrylamide in food samples”. Food chemistry 274 (2019): 55-60.
  12. Zhang Y., et al. “Ultra high-performance liquid chromatography-tandem mass spectrometry for the simultaneous analysis of asparagine, sugars, and acrylamide in maillard reactions”. Analytical Chemistry 83.9 (2011): 3297-3304.
  13. De Paola EL., et al. “Determination of acrylamide in dried fruits and edible seeds using QuEChERS extraction and LC separation with MS detection”. Food Chemistry 217 (2017): 191-195.
  14. Fernández A., et al. “Evaluation of phenolics and acrylamide and their bioavailability in high hydrostatic pressure treated and fried table olives”. Journal of Food Processing and Preservation4 (2020): e14384.
  15. Cagliero C., et al. “Determination of acrylamide in brewed coffee and coffee powder using polymeric ionic liquid-based sorbent coatings in solid-phase microextraction coupled to gas chromatography-mass spectrometry”. Journal of Chromatography A 1449 (2016): 2-7.
  16. Rasooly A and Herold KE. “Biosensors for the analysis of food-and waterborne pathogens and their toxins”. Journal of AOAC International3 (2006): 873-883.
  17. Wu D., et al. “Recent progress on nanomaterial-based biosensors for veterinary drug residues in animal-derived food”. TrAC Trends in Analytical Chemistry 83 (2016): 95-101.
  18. Bitton G and Dutka BJ. “Introduction and review of microbial and biochemical toxicity screening procedures”. Toxicity Testing Using Microorganisms: CRC Press (2019): 1-8.
  19. Baldwin TO and Ziegler MM. “The biochemistry and molecular biology of bacterial bioluminescence”. Chemistry and Biochemistry of Flavoenzymes: CRC Press (2019): 467-530.
  20. Stewart G and Williams P. “Lux genes and the applications of bacterial bioluminescence”. Journal of General Microbiology7 (1992): 1289-1300.
  21. Chatterjee J and Meighen EA. “Biotechnological applications of bacterial bioluminescence (lux) genes”. Photochemistry and Photobiology4 (1995): 641-650.
  22. Ramesh C and Mohanraju R. “Isolation and characterization of marine bioluminescent bacteria for toxicity bioassays and biotechnological applications”. Brazilian Journal of Microbiology3 (2021): 1191-1199.
  23. Van Dyk TK., et al. “Rapid and sensitive pollutant detection by induction of heat shock gene-bioluminescence gene fusions”. Applied and Environmental Microbiology5 (1994): 1414-1420.
  24. Ramanathan S., et al. “Bacterial biosensors for monitoring toxic metals”. Trends in Biotechnology12 (1997): 500-506.
  25. Buchinger S., et al. “Evaluation of chrono-amperometric signal detection for the analysis of genotoxicity by a whole cell biosensor”. Analytica Chimica Acta1-2 (2010): 122-128.

Van Dyk TK. “Bioluminescent gene fusions for characterization of xenobiotic-induced gene expression changes in Escherichia coli: University of Delaware” (1998).

  1. Fukushima K. “Bioactivity of Ephedra: integrating cytotoxicity assessment with real-time biosensing: University of Maryland, College Park” (2004).
  2. Van Dyk T., et al. “A panel of bioluminescent biosensors for characterization of chemically-induced bacterial stress responses”. ACS Publications (2000).

Citation

Citation: Maryam Ganjavi and Yangming Martin Lo. “Identification of Stress Fingerprints Induced by Acrylamide, a Toxic Contaminant in Starchy Food Products, Using Bacterial Bioluminescence". Acta Scientific Nutritional Health 9.4 (2025): 03-07.

Copyright

Copyright: © 2025 Maryam Ganjavi and Yangming Martin Lo. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




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