Maryam Ganjavi^1,3* and Yangming Martin Lo^2,3

¹Nutritional Sciences Program, Department of Public and Allied health, School of Community Health & Policy, Morgan State University, Baltimore, MD, USA
²Institute for Advanced Study, Shenzhen University, Shenzhen, China
³Department of Nutrition and Food Science, College of Agriculture and Natural Resources, University of Maryland, College Park, MD, USA

*Corresponding Author: Maryam Ganjavi, Nutritional Sciences Program, Department of Public and Allied health, School of Community Health & Policy, Morgan State University, Baltimore and Department of Nutrition and Food Science, College of Agriculture and Natural Resources, University of Maryland, College Park, MD, USA.

Received: February 18, 2025; Published: March 04, 2025

Abstract

The presence of acrylamide in food is recognized as a significant concern to human health based on its ability to induce cancer and heritable mutations in laboratory animals. This study aimed to characterize the cellular-level damage of acrylamide by bioluminescence stress fingerprinting. Five genetically engineered strains containing selected stress-responsive E. coli promoters fused to the lux CDABE reporter were employed. Results showed that Luminous E. coli DPD2222, having DNA damage responsive promoter, recA, yielded the highest response, followed by luminous E. coli DPD2234, which contained protein damage responsive promoter, grpE. Quantitative and fingerprint assessment of acrylamide damage could be achieved by optimizing bioluminescence cells constructed with different stress-responsive reporter plasmids.

Keywords: Acrylamide; Luminous Bacteria; Biosensor; E. Coli; Stress Damage

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Citation

Citation: Maryam Ganjavi and Yangming Martin Lo. “Identification of Stress Fingerprints Induced by Acrylamide, a Toxic Contaminant in Starchy Food Products, Using Bacterial Bioluminescence". Acta Scientific Nutritional Health 9.4 (2025): 03-07.

Copyright

Copyright: © 2025 Maryam Ganjavi and Yangming Martin Lo. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.