Exploring the Immunoglobulin Y (IgY) Technology for the Production of Polyclonal Antibodies for Targeted Pathogens of Livestock for Possible Development of Biomolecules in Disease Diagnostics
Kekungu-u Puro1*, Rebecca Marbaniangm2#, Heibor Shilla1, Sabia Khan1, Zakir Hussain1, Umjer Ramshon1, Arun Prince Milton1, Samir Das1 and Sandeep Ghatak1
1Division of Animal and Fisheries Sciences, ICAR-RC for NEH Region, Umiam, Meghalaya, India
2Department of Biotechnology, Faculty of Science and Humanities, SRM Institute of Science and Technology, Chennai, India
*Corresponding Author: Kekungu-u Puro, Division of Animal and Fisheries Sciences, ICAR-RC for NEH Region, Umiam, Meghalaya, India.
Received:
January 27, 2025; Published: February 18, 2025
Abstract
Exploring Immunoglobulin Y (IgY) technology for the production of polyclonal antibodies offers exciting possibilities, particularly in the context of livestock health and disease diagnosis. IgY is the primary antibody found in the egg yolk of birds, such as chickens and presents several advantages over traditional mammalian antibodies. We presented here the production of IgY antibodies in eggs of hyperimmunized rainbow rooster birds against heat inactivated Brucella organism (Brucella abortus and Brucella melitensis) which is an economically important pathogens for livestock with zoonotic implications. The IgY protein extracted from the yolk of immunized eggs was found to be in the range from 1.016mg ml-1 to 4.493 mg ml-1 concentration. The anti-brucella IgY produced can suitably be use to explore and developing an affordable diagnostic kit to detect brucella antigen for screening of brucellosis in livestock
Keywords: Antibodies; Brucellosis; Diagnosis; Immunoglobulin
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