Khasan Kayumov¹*, Lubov Kuchkarova¹ and Komila Eshbakova²

¹National University of Uzbekistan 4 University Street, Tashkent, Uzbekistan
²Institute of the Chemistry of Plant Substances of the Academy of Sciences of the Republic of Uzbekistan, Uzbekistan

*Corresponding Author: Khasan Kayumov; National University of Uzbekistan 4 University Street, Tashkent, Uzbekistan.

Received: November 20, 2023; Published: January 04, 2024

Abstract

With the L-arginine model of experimental pancreatitis (EP), hyperproteinemia, hyperlipidemia, hyperglycemia, hypercholesterolemia, a sharp increase in the activity of digestive hydrolases (α-amylase, lipase, protease complex, active phosphatase) occur. The introduction of flavonoids before the onset of the disease reduced the number of organic substrates and the secretion of hydrolytic enzymes. Therefore, the dysfunction of acinar cells in the pancreas is reduced under the influence of acute pancreatitis. Dihydroquercetin (DHQ) and Rutins (Rt) showed a more effective antipancreatic effect than Pulicaron (Pl) and Tamiflazid (Tm). During EP, the activity of α-glucosidases (maltase, sucrase) decreased in the mucous membrane of the small intestine, while the activity of β-galactosidase (lactase) did not change. The introduction of Rt, DHQ, Pl, and Tm before EP induction led to the fact that the activity of maltase and sucrase approached normal levels in the mucous membrane and cavity of the small intestine, i.e., in the chyme. The rate of glucose transfer from the small intestine into the blood from the carbohydrate polymer, dimer, and monomers was different and was expressed at the highest level in disaccharides (maltose and sucrose). In EP, the transition of glucose from solutions of starch, maltose, sucrose, lactose and glucose into the blood decreases depending on the time of incubation in the small intestine. The introduction of flavonoids before the induction of EP increases the transport of glucose into the blood from almost all substrates. Rt and DHQ have a more stimulating effect on the transport of glucose from carbohydrate substrates into the blood, in contrast to Pl and Tm. With EP, occurs edema, infiltration and inflammation, and vacuole degeneration in the pancreatic acini. The constriction of blood vessels observed during the prophylactic effect of Rt, DHQ, and Tm prevented interstitial tissue edema, vacuolization, and general structural tissue disorders. In contrast to Rt, DHQ, and Tm, the prophylactic effect of Pl on the histostructure of the pancreas was not expressed at all.

Keywords: Experimental Acute Pancreatitis; L-Arginine, Dihydroquercetin; Rutin; Pulicaron; Thamiflaside; Α- Amylase; Acute Pancreatit; Glucose; Histology; Pancreatic Tissue

References

1. Ouyang G., et al. “The global, regional, and national burden of pancreatitis in 195 countries and territories, 1990-2017: a systematic analysis for the Global Burden of Disease Study 2017”. BMC Medicine 18 (2020): 388.
2. Jordan P Iannuzzi., et al. “Global Incidence of Acute Pancreatitis Is Increasing Over Time: A Systematic Review and Meta-Analysis”. Original research full report: Clinical—pancreas1 (2022): 122-134.
3. J Lakshmi Prasanna., et al. “Malabsorption of drugs in gastrointestinal diseases: A Review”. Research Journal of Pharmacy and Technology 1 (2013): 29-33
4. Peery AF., et al. “Burden of gastrointestinal, liver, and pancreatic diseases in the United States”. Gastroenterology 149 (2015): 1731-17
5. Schepers NJ., et al. “Impact of characteristics of organ failure and infected necrosis on mortality in necrotising pancreatitis”. Gut 68 (2019): 1044-10
6. van Santvoort HC., et al. “A conservative and minimally invasive approach to necrotizing pancreatitis improves outcome”. Gastroenterology 141 (2011): 1254-1263.
7. Bang JY., et al. “Superiority of endoscopic interventions over minimally invasive surgery for infected necrotizing pancreatitis: meta-analysis of randomized trials”. Digestive Endoscopy 32 (2020): 298-308.
8. Roberts SE., et al. “The incidence of acute pancreatitis: impact of social deprivation, alcohol consumption, seasonal and demographic factors”. Alimentary Pharmacology and Therapeutics 38 (2013): 539-548.
9. Floyd A., et al. “Secular trends in incidence and 30-day case fatality of acute pancreatitis in North Jutland County, Denmark: a register-based study from 1981-2000”. Scandinavian Journal of Gastroenterology 37 (2009): 1461-1465.
10. Thompson BS., et al. “Prodromal Signs and Symptoms of Chronic Pancreatitis: A Systematic Review”. Journal of Clinical Gastroenterology1 (2022): e1-e10.
11. Ji Hoon Yu and Hyeyoung Kim. “Oxidative stress and inflammatory signaling in cerulein pancreatitis World Journal of Gastroenterology46 (2014): 17324-17329.
12. Zhi Zheng., et al. “A narrative review of acute pancreatitis and its diagnosis, pathogenetic mechanism, and management”. Annals of Translational Medicine's1 (2021): 69.
13. S Khanna and J C Vij. “Severe acute pancreatitis due to hepatitis A virus infection in a patient of acute viral hepatitis Case Reports”. Tropical Gastroenterology1 (2003): 25-26.
14. Kim Myoung-Jin MD., et al. “Silymarin Attenuates the Severity of Cerulein-Induced Acute Pancreatitis”. Pancreas 49.1 (2020): 89-95.
15. Peter Hegyi., et al. “L-arginine-induced experimental pancreatitis”. World Journal of Gastroenterology 10.14 (2004): 2003-2009.
16. R E Banks., et al. “Alpha 2 macroglobulin state in acute pancreatitis. Raised values of alpha 2 macroglobulin-protease complexes in severe and mild attacks”. Gut 32.4 (1991): 430-434.
17. Ajay Dixit., et al. “Role of trypsinogen activation in genesis of pancreatitis”. Pancreapedia: Exocrine Pancreas Knowledge Base (2016).
18. Oleksiy Gryshchenko., et al. “Calcium signalling in the acinar environment of the exocrine pancreas: physiology and pathophysiology”. Journal of Physiology14 (2018): 2663-2678.
19. Srinath Chinnakotla., et al. “Total pancreatectomy and islet autotransplantation in children for chronic pancreatitis: indication, surgical techniques, postoperative management, and long-term outcomes”. Annals of Surgery1 (2014): 56-64.
20. Himesh Soni., et al. “Antimicrobial and Antiinflammatory Activity of the Hydrogels Containing Rutin Delivery”. Asian Journal of Chemistry15 (2013): 8371-8373.
21. Yang X., et al. “Experimental Acute Pancreatitis Models: History, Current Status, and Role in Translational Research”. Frontiers in Physiology 23 (2020).
22. Kahale VP., et al. “To access the efficacy of rutin on 6-hydroxydopamine induced animal model of memory impairment in Parkinson's disease”. Research Journal of Pharmacology and Pharmacodynamics6 (2013): 331-336.
23. Beatriz Gullón Thelmo A., et al. “Rutin: A review on extraction, identification and purification methods, biological activities and approaches to enhance its bioavailability”. 67 (2017): 220-235.
24. KH Kim., et al. “Optimal recovery of high-purity rutin crystals from the whole plant of Fagopyrum esculentum Moench (buckwheat) by extraction, fractionation, and recrystallization”. Bioresource Technology 96 (2005): 1709-1712.
25. Lee KJ., et al. Evidence-Based Complementary and Alternative Medicine (2015): 165457.
26. Chobot V., et al. Molecules12 (2014): 20023-20033.
27. S Karakurt. “Modulatory effects of rutin on the expression of cytochrome P450s and antioxidant enzymes in human hepatoma cells”. Acta Pharmaceutica 66 (2016): 491-502.
28. N Kamalakkannan and SMP Prince. “Rutin improves the antioxidant status in streptozotocin-induced diabetic rat tissues”. Molecular and Cellular Biochemistry 293 (2006): 211-219.
29. Kumar BS., et al. “Estimation of Rutin and Quercetin in Terminalia chebula by HPLC”. Asian Journal of Research in Chemistry4 (2009): 388-389.
30. M Rabiskova., et al. “Coated chitosan pellets containing rutin intended for the treatment of inflammatory bowel disease: In vitro characteristics and in vivo evaluation”. International Journal of Pharmaceutics 422 (2012): 151-159.
31. Himesh Soni., et al. “Antimicrobial and Antiinflammatory Activity of the Hydrogels Containing Rutin Delivery”. Asian Journal of Chemistry15 (2013): 8371-8373.
32. S Faujdar., et al. “Phytochemical evaluation and anti-hemorrhoidal activity of bark of “Acacia ferruginea DC”. Journal of Traditional and Complementary Medicine 9 (2019): 85-89.
33. Kori Yashpal S., et al. “Antihaemorrhoid activity of isolated and semi-synthesized rutin derivative from Euphorbia hirta Linn”. Research Journal of Pharmacy and Technology3 (2020): 1333-1338.
34. Kahale VP., et al. “To access the efficacy of rutin on 6-hydroxydopamine induced animal model of memory impairment in Parkinson's disease”. Research Journal of Pharmacology and Pharmacodynamics6 (2013): 331-336.
35. Choi KS., et al. “Rutin inhibits UVB radiation-induced expression of COX-2 and iNOS in hairless mouse skin: p38 MAP kinase and JNK as potential targets”. Archives of Biochemistry and Biophysics 559 (2014): 38-45.
36. Xu PX., et al. “Rutin improves spatial memory in Alzheimer's disease transgenic mice by reducing A? oligomer level and attenuating oxidative stress and neuroinflammation”. Behavioural Brain Research 264 (2014): 173-180.
37. SV Orlova., et al. “Bioavailability and Safety of Dihydroquercetin (Review)”. Pharmaceutical Chemistry Journal 55 (2022): 1133-1137.
38. Radik A Zaynullin., et al. Medicinal Chemistry Research3 (2017).
39. Efficacy and Safety Study of Diquertin Preparation: Final Report [in Russian], NPO VILAR (1999).
40. B Havsteen. Biochemical Pharmacology 2 (1983): 1141-1148.
41. C Rice-Evans., et al. Trends in Plant Science 2 (1997): 152-159.
42. NV Kondakova., et al. Biol., Med. Farm. Khim. 4 (2002): 46-49.
43. NV Kondakova., et al. Vys. Energ., 32.2 (1998): 106-111.
44. Weidmann AE. “Dihydroquercetin: More than just an impurity?” European Journal of Pharmacology 684 (2012): 19-26.
45. Chen Y and Deuster P. “Comparison of quercetin and dihydroquercetin: Antioxidant-independent actions on erythrocyte and platelet membrane”. Chemico-Biological Interactions 182 (2009): 7-12.
46. Chisolm GM and Steinberg D. “The oxidative modification hypothesis of atherogenesis: An overview”. Free Radical Biology and Medicine 28 (2000): 1815-1826.
47. Marty AT. “The complete german commission e monographs: Therapeutic guide to herbal medicines”. JAMA 281 (1999): 1852-1853.
48. VV Tatarinov., et al. Alfavit 21 (2021): 28-32.
49. V Voskoboinikova., et al. Phytotheraphy Research2 (1993): 208-210.
50. VV Tatarinov., et al. “ Alfavit, 21 (2021): 28-32.
51. VV Tatarinov., et al. “ Alfavit 21 (2021): 28-32.
52. AG Schauss., et al. International Journal of Toxicology2 (2015): 162-181.
53. MB Plotnikov., et al. “Drugs Based on Diquertin [in Russian], Izd”. Tomsk. Univ., Tomsk (2005): 228.
54. D Turck., et al. EFSA Journal2 (2017): e04682.
55. Yu P Fomichev., et al. “Dihydroquercetin and Arabinogalactan-Natural Bioregulators in Human and Animal Vital Activity, Use in Agriculture and Food Industry, Nauchnaya Biblioteka, Moscow” (2017): 702.
56. AA Shkarenkov., et al. Biol. Med. Fam. Khim . 3 (1998): 36-39.
57. GOST 33504-2015. “Food Additives. Dihydroquercetin. Technical Specifications [in Russian], Standartinform, Moscow (2016): 17.
58. A. Bizyuk and M. P. Korolevich, Lech. Delo: Nauchn.-Prakt. Ter. Zh., No. 1, (2013): 13 – 19.
59. D Turck., et al. European Food Safety Authority Journal.15.2 (2017): e04682.
60. R Svobodova., et al. Photochemistry and Photobiology 5 (2017): 1240-1247.
61. RP Terekhov., et al. Molecules22 (2020): 5437.
62. VV Tatarinov., et al. Alfavit 21 (2021): 28-32.
63. YuS Tarakhovskii., et al. “Flavonoids: Biochemistry, Biophysics, Medicine [in Russian]”. Synchrobook, Pushchino (2013): 310.
64. RP Terekhov., et al. “Acta Crystallographica Section B”. Crystal Engineering and Materials2 (2019): 175-182.
65. RP Terekhov and IA Selivanova Vopr. Biol., Med. Farm. Khim 21 (2018): 17-22.
66. VV Tatarinov., et al. Med. Alfavit 21 (2021): 28-32.
67. Wang., et al. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 877.18-19 (2009): 1778-1786.
68. VP Zinchenko., et al. Biofizika, 56.3 (2011): 433-438.
69. CJ Yang., et al. Molecules 21.4 (2016): 494.
70. P. Zinchenko, Yu. A. Kim, Yu. S. Tarakhovskii, and G. E. Bronnikov, Biophysics, 56, No. 3, 433-438 (2011)
71. Y Zu., et al. J. Pharm 477.1-2 (2014): 148-158.
72. RP Terekhov., et al. Molecules22 (2020): 5437 (2020).
73. RP Terekhov., et al. Acta Crystallogr., Sect. B: Struct. Sci., Cryst. Eng. Mater., 75.2 (2019): 175-182.
74. RP Terekhov and I. A. Selivanova, Vopr. Biol., Med. Farm. Khim 21.3 (2018): 17-21.
75. A Selivanova and R. P. Terekhov, Izv. Akad. Nauk, Ser. Khim 12 (2019): 2155-2162.
76. ShO Zokirova., et al. “Dosage form of the drug pulicaron Scientific-practical conference of young scientists dedicated to the 110^th anniversary of Academician S.Yu. Yunusov”. "Actual Problems of the Chemistry of Natural Compounds"
77. Zokirova ShO., et al. “Technology of obtaining dry extract from ground part of pulicaria gnaphalodes l. By percolating extraction method”. World Journal of Pharmaceutical and Medical Research5 (2020): 19-21.
78. Zokirova ShO., et al. “Technology of obtaining dry extract from ground part of pulicaria gnaphalodes l. By percolating extraction method”. World Journal of Pharmaceutical and Medical Research5 (2020): 19-21.
79. R Shakirov., et al. Chemistry of Natural Compounds 32 (1996): 102
80. AS Gromova., et al. Chemistry of Natural Compounds 30 (1994): 363
81. Shakhnoza S. “Azimova Natural Compounds Alkaloids Plant Sources, Structure and Properties” Springer Science (2013).
82. Komilov BD., et al. “NEW FLAVONOID GLYCOSIDE FROM Thalictrum minus”. Chemistry of Natural Compounds5 (2020).
83. https://www.plantarium.ru/lang/en/page/image/id/52798.html
84. Komilov BD.,et al. “NEW FLAVONOID GLYCOSIDE FROM Thalictrum minus”. Chemistry of Natural Compounds5 (2020).
85. Komilov BD., et al. “NEW FLAVONOID GLYCOSIDE FROM Thalictrum minus”. Chemistry of Natural Compounds5 (2020).
86. Jin J Hyun and Sik H Lee. “Experimental Models of Pancreatitis”. Clinical Endoscopy3 (2014).
87. Dawra R and Saluja K “L-arginine-induced experimental acute pancreatitis”. Pancreapedia: Exocrine Pancreas Knowledge Base.
88. Manohar M., et al. “Pathogenic mechanisms of pancreatitis”. World Journal of Gastrointestinal Pharmacology and Therapeutics1 (2017): 10-25.
89. Bohn T. “Dietary factors affecting polyphenol bioavailability”. Nutrition Reviews7 (2014): 429-452.
90. Tarahovsky YS., et al. “Rafts making and rafts braking: how plant flavonoids may control membrane heterogeneity”. Molecular and Cellular Biochemistry 314 (2008): 65-71.
91. Chamara Basnayake and Dilip Ratnam. “Blood tests for acute pancreatitis”. Australian Prescriber4 (2015): 128-130.
92. Lippi G., et al. “Laboratory diagnosis of acute pancreatitis: in search of the Holy Grail”. Critical Reviews in Clinical Laboratory Sciences 49 (2012): 18-31.
93. Banks PA.,et al. “Acute Pancreatitis Classification Working Group . Classification of acute pancreatitis--2012: revision of the Atlanta classification and definitions by international consensus”. Gut 62 (2013): 102-111.
94. Yadav D., et al. “A critical evaluation of laboratory tests in acute pancreatitis”. American Journal of Gastroenterology 97 (2002): 1309-1318.
95. Kimie Date., et al. “Pancreatic α-Amylase Controls Glucose Assimilation by Duodenal Retrieval through N-Glycan-specific Binding, Endocytosis, and Degradation”. Journal of Biological Chemistry28.28 (): 17439-17450.
96. Nickavar B and Abolhasani L. “Bioactivity-guided separation of an α-amylase inhibitor flavonoid from Salvia virgata”. Iranian Journal of Pharmaceutical Research 12 (2013): 57-61.
97. Kimie Date. “Regulatory Functions of α-Amylase in the Small Intestine Other than Starch Digestion: α-Glucosidase Activity, Glucose Absorption, Cell Proliferation, and Differentiation”. New Insights Into Metabolic Syndrome IntechOpen (2020).
98. W R Matull., et al. “Biochemical markers of acute pancreatitis”. The Journal of Clinical Pathology4 (2006): 340-344.
99. Abreu FF., et al. “Elucidating the role of oxidative stress in the therapeutic effect of rutin on experimental acute pancreatitis”. Free Radical Research12 (2016): 1350-1360.
100. Yao Tang., et al. “Phytochemicals with protective effects against acute pancreatitis: a review of recent literature”. Pharmaceutical Biology1 (2022): 479-490.

Citation

Citation: Khasan Kayumov., et al. “General Preventive Effect of Flavonoids Isolated from Plants at Experimental Acute Pancreatitis".Acta Scientific Nutritional Health 8.2 (2024): 15-26.

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Copyright: © 2024 Khasan Kayumov., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.