Acta Scientific Nutritional Health (ASNH)(ISSN: 2582-1423)

Research Article Volume 8 Issue 1

Measuring the Rate of Fungal Surface Area Spread in Homogenous Commercial and Heterogenous Non-Commercial Media

Jasraunaq Singh, Harish Jonnalagedda, Mihir Pargaonkar, Shreya Saran, Shanmukha Priya and Neetu Kalra*

School of Biosciences Engineering Technology, Vellore Institute of Technology, Bhopal, India

*Corresponding Author: Neetu Kalra, School of Biosciences Engineering Technology, Vellore Institute of Technology, Bhopal, India.

Received: November 20, 2023; Published: December 16, 2023

Abstract

The effect of various nutritional factors on fungal growth in cultures made from over-the-counter perishable items (Nutritional Heterogeneity) was explored in the current set of research, and results were compared with lab-grade/commercial media properly calibrated for its growth. Agaricus bisporus (white button mushroom), an edible basidiomycete (filamentous fungi), was chosen for this purpose because it's easily available along with many other favorable properties. A trade-off between the generation of exoenzymes (for an extracellular breakdown of organic material in nitrogen-lacking settings) or sporulation and its mycelium expansion determines the growth efficiency (amount of biomass generated per unit substrate) of fungal cultures. Fungal Growth and a reduced carbon-to-nitrogen ratio are positively correlated [1]. Growth discrepancies (measured by the rate of increase of surface area) in different noncommercial media can also be explained by the presence of various carbohydrate sources, nitrogen sources, and other compounds. We tested the fungal Growth in both organic (glycine) and inorganic source of nitrogen.

Keywords: Agaricus bisporus; Fungal Growth Media; Non-Commercial; Carbon To Nitrogen Ratio; Organic/Inorganic Source Of Nitrogen; Surface Area

References

  1. PD Lonardo., et al. “Effect of nitrogen on fungal growth efficiency”. Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology4 (2020).
  2. DJ Bueno and JO Silva. “The Fungal Hyphae”.
  3. J Rousk and E Baath. “Fungal and bacterial Growth in soil with plant materials of different C/N ratios”. FEMS Microbiology Ecology (2007).
  4. J Macheleidt., et al. “Regulation and Role of Fungal Secondary Metabolites”. Annual Review of Genetics 50 (2006): 371-392.
  5. JC Nielsen., et al. “Global analysis of biosynthetic gene clusters reveals the vast potential of secondary metabolite production in Penicillium specie”. Nature Microbiology (2017).
  6. T Volk. “Fungi At: Encyclopedia of Biodiversity. Second Edition (2013).
  7. N Venkatesh and NP Keller. “Mycotoxins in Conversation With Bacteria and Fungi”. Frontiers in Microbiology (2019).
  8. SO Omeike., et al. “Potential antibiotic-producing fungal strains isolated from pharmaceutical waste sludge”. Beni-Suef University Journal of Basic and Applied Sciences (2019).
  9. A Antecka., et al. “Morphology engineering of basidiomycetes for improved laccase biosynthesis”. Biotechnology Letters 38 (2016): 667-672.
  10. M Umar and L Griensven. “Studies on the morphogenesis of Agaricus bisporus: the dilemma of normal versus abnormal fruit body development”. Mycological Research10 (1999): 1235-1244.
  11. C Semighini and S Harris. “Regulation of Apical Dominance in Aspergillus nidulans Hyphae by Reactive Oxygen Species”. Genetics7 (2008): 1919-1932.
  12. C Booth., et al. “Fungal culture media”. Methods in Microbiology 4 (1971): 49-94.
  13. R Pandey and G Sharma. “Influence of culture media on Growth, colony character and sporulation of fungi isolated from decaying vegetable”. Journal of Yeast and Fungal Research8 (2010): 157-164.
  14. MJ Jeger., et al. “A fungal growth model fitted to carbon-limited dynamics of Rhizoctonia solani”. New Phytologist (2024).
  15. FA Davidson., et al. “Mathematical modeling of fungal Growth and function”. IMA Fungus 2 (2011): 33-37.
  16. Li GAO., et al. “Effects of carbon concentration and carbon to nitrogen ratio on the Growth and sporulation of several biocontrol fungi”. Mycological Research1 (2007): 87-92.
  17. G Braga., et al. “Protease Production during Growth and Autolysis of submerged Metarhizium Anisopliae Cultures”. Environmental Microbiology 2 (1999).
  18. M Mooshammer., et al. “Adjustment of Microbial Nitrogen use Efficiency to Carbon:Nitrogen imbalances regulates soil Nitrogen Cycling”. Nature Communication5 (2014): 3694.
  19. F Vriesa., et al. “Fungal/Bacterial Ratios in grasslands with contrasting Nitrogen Management”. Soil Biology and Biochemistry 8 (2006): 2092-2103.
  20. Lauren C Cline., et al. “Organic nitrogen addition suppresses fungal richness and alters community composition in temperate forest soils”. Environment International 130 (2019): 104913.
  21. A Willetts. “Growth of Penicillium Janthinellum on Glycine as sole Carbon and Nitrogen source”. Biochimica et Biophysica Acta (BBA) - General Subjects3 (1980): 454-463
  22. Alana L DIGBY., et al. “Some fungi in the Chytridiomycota can assimilate both inorganic and organic sources of nitrogen”. IMA Fungus (2021).
  23. Hans Ronne. “Glucose Repression In Fungi. Trends in Genetics1 (1995): 12-17.

Citation

Citation: Neetu Kalra., et al. “Measuring the Rate of Fungal Surface Area Spread in Homogenous Commercial and Heterogenous Non-Commercial Media".Acta Scientific Nutritional Health 8.1 (2024): 34-40.

Copyright

Copyright: © 2024 Neetu Kalra., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




Metrics

Acceptance rate30%
Acceptance to publication20-30 days
Impact Factor1.316

Indexed In





News and Events


  • Certification for Review
    Acta Scientific certifies the Editors/reviewers for their review done towards the assigned articles of the respective journals.
  • Submission Timeline for Upcoming Issue
    The last date for submission of articles for regular Issues is July 10, 2024.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Article of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of "Best Article of the Issue"
  • Welcoming Article Submission
    Acta Scientific delightfully welcomes active researchers for submission of articles towards the upcoming issue of respective journals.

Contact US