Acta Scientific Nutritional Health (ASNH)(ISSN: 2582-1423)

Research Article Volume 8 Issue 1

Nutritional Value, Phytochemical Composition, Toxicological Properties and Antiulcer Action of the Aqueous Extract from Pinus halepensis Mill. Needles in Swiss Mice

Saber Jedidi1,2*, Foued Aloui2, Houcine Selmi2, Dalinda Marzougui1 and Hichem Sebai1

1Laboratory of Functional Physiology and Valorization of Bio-Resources, University of Jendouba, Higher Institute of Biotechnology of Béja, Tunisia
2Laboratory of Sylvo-Pastoral Resources, Institution of Agricultural Research and Higher Education (IRESA), University of Jendouba, Sylvo-Pastoral Institute of Tabarka, Tabarka, Tunisia

*Corresponding Author: Dr. Saber Jedidi, Laboratory of Functional Physiology and Valorization of Bio-Resources, University of Jendouba, Higher Institute of Biotechnology of Béja, Tunisia.

Received: November 23, 2023; Published: December 08, 2023


Background and objectives: Pinus halepensis Mill. (Pinaceae) is commonly used in Tunisian traditional medicine to treat gastrointestinal disorders, including diarrhea and ulcers. The aim of this study was to investigate the toxicological and gastroprotective properties of the aqueous extract from Pinus halepensis Mill. needles (AEPHN) against ethanol-induced gastric ulcer in mice.

Methodology: The phytochemical/antioxidant properties were analyzed by colorimetric/biochemical methods. The toxicological properties of AEPHN were tested with concentrations ranging from 50 to 3000 mg/kg b.w. To study the gastroprotective action of AEPHN, male mouse were divided into five groups of ten animals in each: control (C), ethanol (EtOH), and EtOH + various doses of AEPHN (75, 150, and 300 mg/kg, b.w., p.o.) were daily administrated for 15 days. The last day, animals were intoxicated by acute administration of absolute EtOH (500 μL/kg, b.w., p.o.). After EtOH intoxication, mice were sacrificed and various physiological and biochemical tests were conducted.

Results: The results demonstrated that AEPHN contained many bioactive compounds with a considerable free radicals scavenging activity. The in vivo studies showed that AEPHN protected against EtOH-induced macroscopic alterations and the secretory profile disturbances. AEPHN significantly corrected the depletion of both enzymatic and non-enzymatic antioxidants. Importantly, AEPHN also regulated the intracellular mediators levels in plasma and gastric mucosal.

Conclusion: Our findings clearly demonstrated that the AEPHN exerted an effective protective effect against EtOH-induced gastric ulcer. These effects could be associated with its antioxidant properties and by opposite effects to the components of the Fenton reaction.

Keywords: Pinus Halepensis; Peptic Ulcer; Oxidative Stress; Intracellular Mediators; Mice


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Citation: Saber Jedidi., et al. “Nutritional Value, Phytochemical Composition, Toxicological Properties and Antiulcer Action of the Aqueous Extract from Pinus halepensis Mill. Needles in Swiss Mice".Acta Scientific Nutritional Health 8.1 (2024): 15-20.


Copyright: © 2024 Saber Jedidi., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


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