Acta Scientific Nutritional Health (ASNH)(ISSN: 2582-1423)

Research Article Volume 8 Issue 1

Nutritional Value, Phytochemical Composition, Toxicological Properties and Antiulcer Action of the Aqueous Extract from Pinus halepensis Mill. Needles in Swiss Mice

Saber Jedidi1,2*, Foued Aloui2, Houcine Selmi2, Dalinda Marzougui1 and Hichem Sebai1

1Laboratory of Functional Physiology and Valorization of Bio-Resources, University of Jendouba, Higher Institute of Biotechnology of Béja, Tunisia
2Laboratory of Sylvo-Pastoral Resources, Institution of Agricultural Research and Higher Education (IRESA), University of Jendouba, Sylvo-Pastoral Institute of Tabarka, Tabarka, Tunisia

*Corresponding Author: Dr. Saber Jedidi, Laboratory of Functional Physiology and Valorization of Bio-Resources, University of Jendouba, Higher Institute of Biotechnology of Béja, Tunisia.

Received: November 23, 2023; Published: December 08, 2023

Abstract

Background and objectives: Pinus halepensis Mill. (Pinaceae) is commonly used in Tunisian traditional medicine to treat gastrointestinal disorders, including diarrhea and ulcers. The aim of this study was to investigate the toxicological and gastroprotective properties of the aqueous extract from Pinus halepensis Mill. needles (AEPHN) against ethanol-induced gastric ulcer in mice.

Methodology: The phytochemical/antioxidant properties were analyzed by colorimetric/biochemical methods. The toxicological properties of AEPHN were tested with concentrations ranging from 50 to 3000 mg/kg b.w. To study the gastroprotective action of AEPHN, male mouse were divided into five groups of ten animals in each: control (C), ethanol (EtOH), and EtOH + various doses of AEPHN (75, 150, and 300 mg/kg, b.w., p.o.) were daily administrated for 15 days. The last day, animals were intoxicated by acute administration of absolute EtOH (500 μL/kg, b.w., p.o.). After EtOH intoxication, mice were sacrificed and various physiological and biochemical tests were conducted.

Results: The results demonstrated that AEPHN contained many bioactive compounds with a considerable free radicals scavenging activity. The in vivo studies showed that AEPHN protected against EtOH-induced macroscopic alterations and the secretory profile disturbances. AEPHN significantly corrected the depletion of both enzymatic and non-enzymatic antioxidants. Importantly, AEPHN also regulated the intracellular mediators levels in plasma and gastric mucosal.

Conclusion: Our findings clearly demonstrated that the AEPHN exerted an effective protective effect against EtOH-induced gastric ulcer. These effects could be associated with its antioxidant properties and by opposite effects to the components of the Fenton reaction.

Keywords: Pinus Halepensis; Peptic Ulcer; Oxidative Stress; Intracellular Mediators; Mice

References

  1. Birchenough GM., et al. “New developments in goblet cell mucus secretion and function”. Mucosal Immunology4 (2015): 712-719.
  2. Jedidi S., et al. “Phytochemical/antioxidant properties and individual/synergistic actions of Salvia officinalis aqueous extract and loperamide on gastrointestinal altering motor function”. Journal of Medicinal Food 22.12 (2019): 1235-1245.
  3. Rtibi K., et al. “Gastroprotective effect of carob (Ceratonia siliqua) against ethanol-induced oxidative stress in rat”. BMC Complementary Medicine and Therapies 15 (2015): 292.
  4. Zatorsk H. “Pathophysiology and risk factors in peptic ulcer disease”. In: Fichna, J. (eds) Introduction to Gastrointestinal Diseases (2017): 2.
  5. Yeomans ND. “The ulcer sleuths: The search for the cause of peptic ulcers”. Journal of Gastroenterology and Hepatology 1 (2011): 35-41.
  6. Li S., et al. “Molecular targets and mechanisms of bioactive peptides against metabolic syndromes”. Food and Function1 (2018): 42-52.
  7. Chakrabarti S., et al. “Food-derived bioactive peptides on inflammation and oxidative stress”. BioMed Research International 2014 (2014): 11.
  8. Jedidi, S., et al. “Antioxidant properties, phytoactive compounds and potential protective action of Salvia officinalis flowers against combined gastro-intestinal ulcer and diarrhea experimentally induced in rat”. Dose-Response2 (2022): 15593258221102313.
  9. Basseri RJ, et al. “Antibiotics for the treatment of irritable bowel syndrome”. Gastroenterology and Hepatology (N Y)7 (2011): 455-493.
  10. Philpott HL., et al. “Drug-induced gastrointestinal disorders”. Frontline Gastroenterology 5 (2014): 49-57.
  11. McQuaid KR. “Drugs used in the treatment of gastrointestinal diseases”. Basic and Clinical Pharmacology 10 (2017): 1029-1035.
  12. Imhann F., et al. “Proton pump inhibitors affect the gut microbiome”. Gut5 (2016): 740-748.
  13. Lakatos PL and Lakatos L. “Risk for colorectal cancer in ulcerative colitis: changes, causes and management strategies”. World Journal OG Gastroenterology25 (2008): 3937-3947.
  14. Gill SK., et al. “Dietary fibre in gastrointestinal health and disease”. Nature Review Gastroenterology and Hepatology 2 (2021): 101-116.
  15. Ayari A., et al. “Geographical variation on cone and seed production of natural Pinus halepensis forests in Tunisia”. Journal of Arid Environments 75.5 (2011): 403-410.
  16. Xie Q., et al. “Chemical composition and antioxidant activity of essential oil of six pinus taxa native to China”. Molecules 5 (2015): 9380-9392.
  17. Politeo O., et al. “Chemical composition and antimicrobial activity of the essential oil of endemic Dalmatian black pine (Pinus nigra dalmatica)”. Chemistry and Biodiversity 8.3 (2011): 540-547.
  18. Karapandzova M., et al. “Chemical composition and antimicrobial activity of the essential oils of Pinus peuce (Pinaceae) growing wild in R. Macedonia”. Natural Product Communications 11 (2014): 1623-1628.
  19. Hajdari A., et al. “Essential oil composition of Pinus peuce Needles and Twigs from Two National Parks of Kosovo”. Scientific World Journal 2016 (2016): 5393079.
  20. Aidi Wannes W., et al. “Chemical profile, allelopathic, antibacterial and antioxidant potential of the essential oil from Aleppo pine (Pinus halepensis Miller) needles”. Archives of Phytopathology and Plant Protection 54 (2021): 2483-
  21. “Official methods of analysis”. Association of official analytical chemists. Washington. DC (1990).
  22. Van Soest PJ and Maraus WC. “Method for the determination of cell wall constituents in forage using detergents and the relationship between this fraction and voluntary intake and digestibility”. Journal of Dairy Science 58 (1994): 704-
  23. Bligh EG and Dyer WJA. “Rapid method of total lipid extraction and purification”. Canadian Journal of Biochemistry and Physiology 37 (1959): 911-917.
  24. Slinkard K and Singleton VL. “Total phenol analysis: automation and comparison with manual methods”. American Journal of Enology and Viticulture 28 (1977): 49-
  25. Hussain A., et al. “Evaluation of the phytochemical and medicinal value of lemongrass (Cymbopogon citratus), by conversion into powders and extracts to develop a nutritional bakery product”. Future Integrative Medicine 3 (2023): 129-140.
  26. Price ML., et al. “A critical evaluation of the Vanillin reaction as an assay for tannin in sorghum grain”. Journal of Agricultural and Food Chemistry5 (1978): 1214-1218.
  27. Elfalleh W., et al. “Physico-chemical properties and DPPH-ABTS scavenging activity of some local pomegranate (Punica granatum) ecotypes”. International Journal of Food Sciences and Nutrition 60 (2009): 197-
  28. Olorunnisola OS., et al. “Toxicity evaluation and protective effect of Rhus longipes Engl. leaf extract in paracetamol induced oxidative stress in wister rats”. Journal of Phytopharmacology 6 (2017): 73-77.
  29. Misra HP, Fridovich I. “The role of superoxide anion in autoxidation of epinephrine and a simple assay for superoxide dismutase”. Journal of Biological Chemistry10 (1972): 3170-3175.
  30. Aebi H. “Catalase in vitro”. Methods of Enzymology 105 (1984): 121-126.
  31. Dingeon B., et al. “Automatic assay of blood sugar by Trinder's method”. Annales de Biologie Clinique1 (1975): 3-13.
  32. Ellman GL. “Tissue sulfhydryl groups”. Archives of Biochemistry and Biophysics1 (1959): 70-77.
  33. Jedidi S., et al. “Comparative studies of phytochemical screening, HPLC-PDA-ESI-MS/MS LC/HRESI/MS analysis, antioxidant capacity and in vitro fermentation of officinal sage (Salvia officinalis) cultivated in different biotopes of Northwestern Tunisia”. Chemistry & Biodiversity 17.1 (2020): e1900394.
  34. Saad-Allah KM, Ragab GA. “Sulfur nanoparticles mediated improvement of salt tolerance in wheat relates to decreasing oxidative stress and regulating metabolic activity”. Physiology and Molecular Biology of Plants 11 (2020): 2209-2223.
  35. Jabri MA., et al. “Role of laxative and antioxidant properties of Malva sylvestris leaves in constipation treatment”. Biomedicine and Pharmacotherapy 89 (2017): 29-35.
  36. Bhardwaj K., et al. “Studies of phytochemicals, antioxidant, and antibacterial activities of Pinus gerardiana and Pinus roxburghii seed extracts”. BioMed Research International 2022 (2022): 5938610.
  37. Refifa T., et al. “Allelopathic potential of Pinus halepensis needles”. Allelopathy Journal2 (2016): 193-214.
  38. Kotroni E., et al. “In vivo evaluation of the anti-inflammatory activity of electrospun micro/nanofibrous patches loaded with Pinus halepensis bark extract on hairless mice skin”. Materials 16 (2019): 2596.
  39. Atmane SA., et al. “Acute and 28-day repeated dose toxicity evaluations of cold pressed Pinus halepensis seed oil in mice and rats”. Regulatory Toxicology Pharmacology 132 (2022): 105191.
  40. Jabri MA., et al. “Fatty acid composition and mechanisms of the protective effects of myrtle berry seed aqueous extract in alcohol-induced peptic ulcer in rat”. Can Journal of Physiology and Pharmacology5 (2017): 510-521.
  41. Liu J., et al. “Protective effects of anwulignan against HCl/Ethanol-induced acute gastric ulcer in mice”. Evidence-Based Complementary and Alternative Medicine 2021 (2021): 9998982.
  42. Takeuchi K. “Gastric cytoprotection by prostaglandin E2 and prostacyclin: relationship to EP1 and IP receptors”. Journal of Physiology and Pharmacology1 (2014): 3-14.
  43. Tarnawski AS., et al. “The mechanisms of gastric mucosal injury: focus on microvascular endothelium as a key target”. Current Medicinal Chemistry1 (2012): 4-15.
  44. Prasad S., et al. “Metal-Curcumin complexes in therapeutics: An approach to enhance pharmacological effects of curcumin”. International Journal of Molecular Sciences13 (2021): 7094.
  45. Ismail Suhaimy NW., et al. “Semipurified ethyl acetate partition of methanolic extract of Melastoma malabathricum leaves exerts gastroprotective activity partly via its antioxidant-antisecretory-anti-inflammatory action and synergistic action of several flavonoid-based compounds”. Oxidative Medicine and Cellular Longevity 2017 (2017): 6542631.
  46. Amang AP., et al. “Gastro-protective activity of the leaves aqueous extract of Diospyros mespiliformis on gastric ulcers in Swiss mice”. International Journal of Pharmacognosy 7 (2020): 44-51.
  47. Rocha Caldas GF., et al. “Gastroprotective Mechanisms of the Monoterpene 1,8-Cineole (Eucalyptol)”. PLoS One 8 (2015): e0134558.
  48. Jedidi S., et al. “Antioxidant properties of Salvia officinalis decoction extract and mechanism of its protective effects on ethanol-induced liver and kidney injuries”. Journal of Medicinal Food5 (2022): 546-556.
  49. Sánchez M., et al. “Iron chemistry at the service of life”. IUBMB Life6 (2017): 382-388.
  50. Pisoschi AM, Pop A. “The role of antioxidants in the chemistry of oxidative stress: A review”. European Journal of Medicinal Chemistry 97 (2015): 55-74.

Citation

Citation: Saber Jedidi., et al. “Nutritional Value, Phytochemical Composition, Toxicological Properties and Antiulcer Action of the Aqueous Extract from Pinus halepensis Mill. Needles in Swiss Mice".Acta Scientific Nutritional Health 8.1 (2024): 15-20.

Copyright

Copyright: © 2024 Saber Jedidi., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




Metrics

Acceptance rate30%
Acceptance to publication20-30 days
Impact Factor1.316

Indexed In





News and Events


  • Certification for Review
    Acta Scientific certifies the Editors/reviewers for their review done towards the assigned articles of the respective journals.
  • Submission Timeline for Upcoming Issue
    The last date for submission of articles for regular Issues is July 10, 2024.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Article of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of "Best Article of the Issue"
  • Welcoming Article Submission
    Acta Scientific delightfully welcomes active researchers for submission of articles towards the upcoming issue of respective journals.

Contact US