Abdullah H Aljohani¹, Abdulelah Aljuaid¹, Mamdouh Allahyani¹, Jood A AL-Kasi², Naif D Althuwaybi¹, Renad N AL-Jaiud¹, Osama Abdulaziz¹, Meshari A Alsuwat¹, Sumaya Albazi², Ahmed S Alsuwat³, Thamer A Althobaiti³, Abeer M Alshehry³, Wedad almotairi⁴ and Mazen M Almehmadi¹*

¹Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Taif University, Taif, Kingdom of Saudi Arabia
²Taif University, TAIF and Blood Bank, Laboratories, and Blood Banks Administration, Taif, Makkah Province, Kingdom of Saudi Arabia
³Laboratory Department, Children Hospital, Saudi Arabia
⁴Directorate of health affairs in Taif, TAIF, Makkah Province, Kingdom of Saudi Arabia

*Corresponding Author: Mazen M Almehmadi, Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Taif University, Taif, Kingdom of Saudi Arabia.

Received: November 17, 2023; Published: November 28, 2023

Abstract

Background: Helicobacter pylori (H. pylori) is a gram-negative-bacilli-bacteria that infects humans' stomachs and the duodenum. H. pylori infection is the primary cause of several disorders, including chronic gastritis, gastric ulcers, duodenal ulcers, and gastric adenocarcinomas. In addition, the prevalence of H. pylori is common. This study aimed to study the phenotypical analysis of peripheral T-cells and γδ-T-cells in H. pylori seropositive individuals.

Methods: A cross-sectional study was conducted on 450 participants at the College of Applied Medical Sciences at Taif University. ELISA was used to screen the participants for H. pylori. Then we select seropositive H. pylori participants for flow cytometry analysis of peripheral T cells and γδ-T-cells.

Results: 24 out of 450 (5.3%) were IgG-positive for H. pylori. We collected another sample to analyze the peripheral T-cells and γδ-T-cells in H. pylori-seropositive participants. The dot plots of CD4 and CD8 showed a slight increase among H. pylori-seropositive participants compared to the control group. At the same time, there was a significant increase in γδ-T-cells.

Conclusion: The current research showed a significant rise in γδ-T-cells, with a slight increase in CD4 and CD8 which were insignificant. Furthermore, among participants who tested seropositive for H. pylori, an increase in γδ-T- cells was observed in those experiencing H. pylori-related complications. This increase may play a crucial role in the excessive production of cytokines, ultimately leading to gastritis, stomach cancer, and the development of tumors.

Keywords: Helicobacter; cd8 t Helper; cd4 t Helper; γδ-t- Cells; h pylori

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Citation

Citation: Mazen M Almehmadi., et al. “Phenotypical Analysis of Peripheral T-Cells and γδ-T-Cells in Helicobacter Pylori Seropositive Individuals".Acta Scientific Nutritional Health 7.12 (2023): 39-44.

Copyright

Copyright: © 2023 Mazen M Almehmadi., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.