Eman E Saafan*

Department of Food Industries, Faculty of Agriculture, Mansoura University, Mansoura, Egypt

*Corresponding Author: Eman E Saafan, Department of Food Industries, Faculty of Agriculture, Mansoura University, Mansoura, Egypt.

Received: September 01, 2023; Published: September 25, 2023

Abstract

The present study is conducted to evaluate the antioxidative activity of Bitter Orange seeds extract on stability of rice bran oil. Ethanolic extract of Bitter Orange seeds was added to rice bran oil (200 and 300 ppm) as natural antioxidant compare with Tertiary Butylhydroxy Quinone (TBHQ) as synthetic one. Oxidative stability and thermal process for 8 hours at 180°C for treated rice bran oil with different antioxidants were estimated. Obtained results indicated that the total phenolic content being 51.94 mg of GAE/g in ethanolic extract of bitter orange seeds, while total flavonoids being 39.67mg RE/g in the same extract. Bitter orange seeds ethanolic extract DPPH activity was nearly to TBHQ. Identification and fractionation of phenolic compounds using HPLC cleared that ten phenolic compounds (371.53 and 11.25 mg/100g) were separated. E-vanillic the most abundant phenolic compound in Bitter Orange seeds extract was, while Hesperidin was the most abundant flavonoids compound in the same extract 929.91 mg/100 g. Results for thermal process of treated rice bran oil by Bitter orange seeds ethanolic extract at the concentration of 300 ppm were recorded the lowest values of hydrolysis, oxidation and rancidity parameter after 8 hours heating. So, addition of Bitter Orange seeds extract to rice bran oil showed a positive effect on the oxidative and thermal stabilities of such raw material and could be recommended as an alternative natural antioxidant from by – products in oil.

Keywords: Bitter Orange Seeds; Antioxidants Activity; Total Phenolic; Flavonods

References

1. Zahra Maqbool., et al. “Citrus Waste as Source of Bioactive Compounds: Extraction and Utilization in Health and Food Industry”. Molecules4 (2023): 1636.
2. Chala Gowe. “Potential Use of Fruit and Vegetables By-Products as A Valuable Source of Natural Food Additives”. Food Science and Quality Management 45 (2015): ISSN 2224-6088.
3. Bursa´c Kovaˇcevi´c D., et al. “Stability of polyphenols in chokeberry juice treated with gas phase plasma”. Food Chem 212 (2016): 323-331.
4. Qiyang Chen., et al. “Profiling of Flavonoid and Antioxidant Activity of Fruit Tissues from 27 Chinese Local Citrus Cultivars”. Plants 9 (2020): 196.
5. El-Gammal Rania E. “Antioxidative Activity of Nanoparticles of Rosemary”. International Journal of ChemTech Research 12 (2016): 844-854.
6. Marmesat S., et al. “Action and Fate of GRASASY ACEITES" 61.4 (2010): 333-340.
7. Semnani KM., et al. “Antioxidant Activity of the Methanolic Extracts of Some Species of Phlolmis and Stachys on Sunflower Oil". African Journal of Biotechnology 24 (2006): 2428- 2432.
8. Aluyor EO and Ori-Jesu M. “The use of antioxidants in vegetable oils-A review”. African Journal of Biotechnology25 (2008): 4836-4842.
9. Ivanova V., et al. “Determination of the polyphenol content in macedonian grapes and wines by standardized spectrophotometric method”. Journal of the Serbian Chemical Society 1 (2011): 45-59.
10. “Association of official analytical chemists official methods of analysis (18^th edition)”. Washington, DC, USA (2011).
11. Goupy P., et al. “Antioxidant Composition and Activity of Barley (Hordeum vulgare) and Malt extracts and Isolated Phenolic Compounds". Journal of the Science of Food and Agriculture 79 (2000): 1625-1634.
12. Brand-Williams W., et al. “Use of Free Radical Method to Evaluate Antioxidant Activity". Lebensmittel-Wissenschaft und -Technologie 28 (1995): 25-30.
13. Almey A., et al. “Total phenolic content and Primary Antioxidants Activity of Methanoic and Ethanolic Extracts of Aromatic Plant's Leaves”. International Food Research Journal 17 (2010): 1077-1084.
14. “Association of official analytical chemists official methods of analysis (18^th edition)”. Washington, DC, USA (2005).
15. Bahman Fazeli-Nasab., et al. “Evaluation of Antioxidant and Antimicrobial Activity of Some Medicinal Plant Extracts on Escherichia coli Isolated from Poultry Feces”. Journal of Medicinal Plants and By-products 13 (2021): 1-11.
16. Fazeli-nasab B., et al. “Effect of Solvent Extraction on Phenol, Flavonoids and Antioxidant Activity of some Iranian Native Herbs”. Scientific Journal of Ilam University of Medical Sciences 3 (2019): 14-26.
17. Bhavaniramya S., et al. “Role of essential oils in food safety: Antimicrobial and antioxidant applications”. Grain and Oil Science and Technology 2 (2019): 49-55.
18. A Sabry., et al. “Effect of using rice bran oil in spreadable fats preparation on quality criteria during cold storage”. Al-Azhar Journal of Agricultural Research2 (2020): 49-61.
19. Suduwa Devage Chamika Sewwandi and Palitha Chandrapema Arampath. “Preparation of Trans Fat Free Bakery Margarine with Rice Bran Oil and Palm Stearin”. World Journal of Food Science and Technology2 (2022): 31-38.
20. Srivastava P and Singh RP. “Frying stability evaluation of rice bran oil blended with soybean, mustard and palm olein oils”. Oriental Journal of Chemistry 3 (2015): 1687-1694.
21. Paul A., et al. “Comparative analysis of heat degradation of oryzanol in rice bran oil, mustard oil and sunflower oil by microwave and pan heating”. International Journal of Food and Nutritional Sciences 1 (2012): 110-117.
22. Askar Mohamed AA. “Utilization of phytosterols in Functional Foods. PHD. Thesis, Fac. Of Agric. Mansoura, univ. Mansoura, Egypt (2017).
23. Jenab E and Temelli F. “Viscosity measurement and modeling of canola oil and its blend with canola stearin in equilibrium with high pressure carbon dioxide”. The Journal of Supercritical Fluids 1 (2011): 7-14.
24. Khalaf YA. “Effect of blending and Natural Antioxidants on stability of sunflower oil. M.S.C. Thesis, Fac. Of Agric. Mansoura, Univ. Mansoura, Egypt (2015).
25. Karoui IJ., et al. “Thermal stability of corn oil flavoured with Thymus Capitatus under heating and deep -frying conditions”. Journal of the Science of Food and Agriculture 5 (2011): 927-933.
26. Saafan Eman E. “Chemical, physical and technological studies on edible vegetable fats blends” Ph.D Thesis, Fac. Of Agric. Mansoura, Univ. Mansoura, Egypt (2014).
27. Azmi NS., et al. “Characterization of antioxidant tapioca starch/polyaniline composites film prepared using solution casting method”. Food Research4 (2019): 317-324.
28. Riantong S and Jorg JJ. “Nutrition and applications of rice bran oil: a mini overview”. Science and Technology of Cereals, Oils and Foods 29.3 (2021): 47-53.

Citation

Citation: Eman E Saafan. “Utilization of Bitter Orange Seeds as by-Product to Produce Natural Antioxidants".Acta Scientific Nutritional Health 7.10 (2023): 56-61.

Copyright

Copyright: © 2023 Eman E Saafan. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.