Acta Scientific Nutritional Health (ASNH)(ISSN: 2582-1423)

Research Article Volume 7 Issue 6

Levi Levilactobacillus brevis (TCI988) Supplementation Enhances Sleep Quality

Ping Lin1, Yung-Hsiang Lin1, Yung-Kai Lin2 and Chi-Fu Chiang1

1Research and Design Center, TCI CO., Ltd., Taipei, Taiwan 2Institute of Food Safety and Risk Management, National Taiwan Ocean University, Keelung, Taiwan and Department of Food Science, National Taiwan Ocean University, Keelung, Taiwan and Graduate Institute of Biomedical Engineering, National Chung Hsing University, Taichung, Taiwan.

*Corresponding Author: Chi-Fu Chiang, Research and Design Center, TCI CO., Ltd., Taipei, Taiwan..

Received: May 05, 2023; Published: May 13, 2023


Levilactobacillus brevis (TCI988) is a probiotic known for improving gut health, enhancing the immune system, and reducing inflammation and improved sleep quality. The aim of this study was to investigate whether L. brevis increased deep sleep and improve stress. Neuro-2a cells were treated with TCI988 for 24 hours, and then examined mitochondrial activity and related melatonin genes. In addition, subjects analyzed the GABA concentration and sleep and stress status. TCI988 can increase neuronal cell mitochondrial activity by 33.4% compared to the mock, and increased the expression of SIRTs, TPH1, and DDC by about 1.3, 1.23, and 1.05-fold, respectively, compared to the mock. The serum GABA level of the subjects increased from 165.89 ng/mL to 217.9 ng/mL after consuming TCI988 for 4 weeks, and increased deep sleep time and improved stress. L. brevis showed promise as a natural remedy for improving sleep quality.

Keywords:Deep Sleep; GABA; Levilactobacillus brevis; Probiotics; Stress


  1. Marotta A., et al. “Effects of Probiotics on Cognitive Reactivity, Mood, and Sleep Quality”. Frontiers in Psychiatry 10 (2019): 164.
  2. Yan F and Polk DB. “Probiotics and immune health”. Current Opinion in Gastroenterology 27 (2011): 496-501.
  3. Gasmi A., et al. “Neurotransmitters Regulation and Food Intake: The Role of Dietary Sources in Neurotransmission”. Molecules (2022): 28.
  4. Barton W., et al. “The microbiome of professional athletes differs from that of more sedentary subjects in composition and particularly at the functional metabolic level”. Gut 67 (2018): 625-633.
  5. Kazmierczak-Siedlecka K., et al. “The use of Lactobacillus plantarum 299v (DSM 9843) in cancer patients receiving home enteral nutrition - study protocol for a randomized, double-blind, and placebo-controlled trial”. Nutrition Journal 19 (2020): 98.
  6. Shahbazi R., et al. “Anti-Inflammatory and Immunomodulatory Properties of Fermented Plant Foods”. Nutrients 13 (2021).
  7. Raj G., et al. “Controlling the dispersion of supported polyoxometalate heterogeneous catalysts: impact of hybridization and the role of hydrophilicity-hydrophobicity balance and supramolecularity”. Beilstein Journal of Nanotechnology 5 (2014): 1749-59.
  8. Satomi S., et al. “Short Communication: Oral Administration of Heat-killed Lactobacillus brevis KB290 in Combination with Retinoic Acid Provides Protection against Influenza Virus Infection in Mice”. Nutrients 12 (2020): 2925.
  9. Jin Y., et al. “Gamma-Aminobutyric Acid-Producing Levilactobacillus brevis Strains as Probiotics in Litchi Juice Fermentation”. 12 (2023): 302.
  10. Olekhnovich EI., et al. “The effects of Levilactobacillus brevis on the physiological parameters and gut microbiota composition of rats subjected to desynchronosis”. Microbial Cell Factories 20 (2021): 226.
  11. Ratanaburee A., et al. “Enhancement of γ-aminobutyric acid in a fermented red seaweed beverage by starter culture Lactobacillus plantarum DW12”. Electronic Journal of Biotechnology 14 (2011): 1.
  12. Tremblay RG., et al. “Differentiation of mouse Neuro 2A cells into dopamine neurons”. Journal of Neuroscience Methods 186 (2010): 60-67.
  13. Sivandzade F., et al. “Analysis of the Mitochondrial Membrane Potential Using the Cationic JC-1 Dye as a Sensitive Fluorescent Probe”. Bio-protocol 9 (2019).
  14. Li Z-y., et al. “The Potential Application of Spring Sargassum glaucescens Extracts in the Moisture-Retention of Keratinocytes and Dermal Fibroblast Regeneration after UVA-Irradiation” 6 (2019): 17.
  15. Lombardi JP., et al. “Visualizing GABA A Receptor Trafficking Dynamics with Fluorogenic Protein Labeling”. Current Protocols in Neuroscience 92 (2020): e97.
  16. Concheiro-Moscoso P., et al. “Use of the Xiaomi Mi Band for sleep monitoring and its influence on the daily life of older people living in a nursing home”. Digital Health 8 (2022): 20552076221121162.
  17. Zlomke KR. “Psychometric properties of internet administered versions of Penn State Worry Questionnaire (PSWQ) and Depression, Anxiety, and Stress Scale (DASS)”. Computers in Human Behavior 25 (2009): 841-843.
  18. Zheng Y., et al. “Probiotics Supplementation Attenuates Inflammation and Oxidative Stress Induced by Chronic Sleep Restriction”. Nutrients 15 (2023).
  19. Auger RR., et al. “Clinical Practice Guideline for the Treatment of Intrinsic Circadian Rhythm Sleep-Wake Disorders: Advanced Sleep-Wake Phase Disorder (ASWPD), Delayed Sleep-Wake Phase Disorder (DSWPD), Non-24-Hour Sleep-Wake Rhythm Disorder (N24SWD), and Irregular Sleep-Wake Rhythm Disorder (ISWRD). An Update for 2015: An American Academy of Sleep Medicine Clinical Practice Guideline”. Journal of Clinical Sleep Medicine 11 (2015): 1199-236.
  20. Kishi A., et al. “Effect of the oral administration of Lactobacillus brevis subsp. coagulans on interferon-alpha producing capacity in humans”. Journal of the American College of Nutrition 15 (1996): 408-412.
  21. Diez-Gutiérrez L., et al. “Gamma-aminobutyric acid and probiotics: Multiple health benefits and their future in the global functional food and nutraceuticals market”. Journal of Functional Foods 64 (2020): 103669.
  22. Li H., et al. “Production of gamma-aminobutyric acid by Lactobacillus brevis NCL912 using fed-batch fermentation”. Microbial Cell Factories 9 (2010): 85.
  23. Ishikawa R., et al. “Dietary heat-killed Lactobacillus brevis SBC8803 (SBL88) improves hippocampus-dependent memory performance and adult hippocampal neurogenesis”. Neuropsychopharmacology Report 39 (2019): 140-145.
  24. Higo-Yamamoto S., et al. “Dietary Heat-Killed Lactobacillus brevis SBC8803 Attenuates Chronic Sleep Disorders Induced by Psychophysiological Stress in Mice”. Journal of Nutritional Science and Vitaminology (Tokyo) 65 (2019): 164-170.
  25. Huang R., et al. “Effect of Probiotics on Depression: A Systematic Review and Meta-Analysis of Randomized Controlled Trials”. Nutrients 8 (2016).
  26. Fukui Y., et al. “Effect of Lactobacillus brevis KB290 on the cell-mediated cytotoxic activity of mouse splenocytes: a DNA microarray analysis”. British Journal of Nutrition 110 (2013): 1617-1629.
  27. Ceppa FA., et al. “Human Gut-Microbiota Interaction in Neurodegenerative Disorders and Current Engineered Tools for Its Modeling”. Frontiers in Cellular and Infection Microbiology 10 (2020): 297.
  28. Kwon YH., et al. “Modulation of Gut Microbiota Composition by Serotonin Signaling Influences Intestinal Immune Response and Susceptibility to Colitis”. Cellular and Molecular Gastroenterology and Hepatology 7 (2019): 709-728.


Citation: Chi-Fu Chiang., et al. “Levi Levilactobacillus brevis (TCI988) Supplementation Enhances Sleep Quality". Acta Scientific Nutritional Health 7.6 (2023): 37-43.


Copyright: © 2023 Chi-Fu Chiang., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Acceptance rate30%
Acceptance to publication20-30 days
Impact Factor1.316

Indexed In

News and Events

  • Certification for Review
    Acta Scientific certifies the Editors/reviewers for their review done towards the assigned articles of the respective journals.
  • Submission Timeline for Upcoming Issue
    The last date for submission of articles for regular Issues is May 30, 2024.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Article of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of "Best Article of the Issue"
  • Welcoming Article Submission
    Acta Scientific delightfully welcomes active researchers for submission of articles towards the upcoming issue of respective journals.

Contact US