Antioxidant Properties of Nine Commonly Used Medicinal Plants in Arabs Region
Abuelgassim Omer Abuelgassim*
Department of Biochemistry, King Saud University, Riyadh, Kingdom of Saudi Arabia
*Corresponding Author: Abuelgassim Omer Abuelgassim, Department of
Biochemistry, King Saud University, Riyadh, Kingdom of Saudi Arabia.
October 25, 2022; Published: November 07, 2022
The present study was conducted to investigate the total phenolics, total flavonoid, and Trolox equivalent antioxidant capacity of 9 commonly used medicinal plants and herbs among Arabs nation namely, Trachyspermum ammi (Apiaceae) seeds, Ferula assa-foetida (Apiaceae) dried latex, Elettaria cardamomum (Zingiberaceae) fruit 1, Senna alexandrina (Caesalpinioideae) Leguminosae leaves, Adenium obesum (Apocynaceae) leaves, Elettaria cardamomum (Zingiberaceae) fruit 2, Trigonella foenum-graecum (Leguminosae) seeds, Lepidium sativum (Brassicaceae) seeds, Ziziphus spina-christi (Rhamnaceae) fruit, and Nigella sativa (Ranunculaceae) seeds.
The methanol extract of each plant was obtained by dissolving 2g of plant powder in 20 ml methanol-water (4:1v/v) to overnight at room temperature. The supernatants were concentrated by using a rotary evaporator. Residues of each extract were dissolved in distilled water and the final volume was recorded. A serial dilution of 10, 100, 1000, and 5000 were made.
The mean values of the total phenolics content were 535.24 ± 70.68, 150.28 ± 36.89, 69.05 ± 14.18, 1358.7 ± 118.88, 605.83 ± 117.67, 228.28 ± 53.8, 2171.57 ± 296.2, 726.65 ± 130.73, 702.63 ± 32.93, and 132.4 ± 30.77 (mg GAE/100g) in T. ammi, F. assa- foetida, E.cardamomum1, S. alexandrina, A. obesum, E.cardamomum2, T. foenum- graecum, L. sativum, Z. spina-christi and N. sativa respectively, whereas the mean values of the total flavonoid content were 143.96 ± 6.44,0.0, 9.16 ± 3.14,761.66 ± 14.71, 210.09 ± 7.61,12.83 ± 0.88, 347 ± 60.15,94.84 ± 10.04, 94.07 ± 6.26, and 30.49 ± 0.33 ( mg QE/100g) respectively. The determined scavenging activity against ABTS radical of the aqueous methanolic extracts of T. ammi, F. assa- foetida, E. cardamomum1, S. alexandrina, A. obesum, E. cardamomum2, T. foenum- graecum, L. sativum, Z. spina-christi and N. sativa were 1271.32 ± 273.35, 234.89 ± 42.2, 135 ± 21.45, 2551.83 ± 305.35, 2101.1 ± 298.97,794.9 ± 175.72, 794.73 ± 149.49, 2219.74 ± 382.12, 3093.11 ± 445.93, and 483.07 ± 98.17 (μmole TEAC/100g) respectively. Among studied plants T. foenum-graecum showed the highest phenolic content (2171.57 ± 296.2 mg GAE/100g) whereas S. alexandrina showed the highest total flavonoid content (761.66 ± 14.71 mg QE/100g). The highest IC50s were noticed in T. ammi, S. alexandrina, Z. spina-christi, and A. obesum extracts (100.035 ± 19.38, 583.38 ± 45.11, 666.87 ± 79.62 and 720.51 ± 92.24μg respectively). The hydromethanolic extracts of T. foenum-graecum, S. alexandrina, A. obesum, L. sativum and Z. spina-christi showed a significant antioxidant property, further research is ongoing to identify their bioactive compounds, in particular phenolic acids. Our findings suggest the possibility of applying T. foenum-graecum, S. alexandrina, A. obesum, L. sativum and Z. spina-christi in food and/or pharmaceutical industries.
Keywords: Total Phenolics; Total Flavonoid; Scavenging Activity; T. Foenum-Graecum; S. Alexandrina; Z. Spina-Christi
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