Acta Scientific Nutritional Health (ASNH)(ISSN: 2582-1423)

Review Article Volume 6 Issue 3

A Retrospective Study of 25(OH)D Levels in Children of Different Age, Sex, and Nationality

Afrozul Haq1*, Jitka Sirohi2, Mohammad Imran3 and William B Grant4

1Department of Food Technology, School of Interdisciplinary Sciences, Jamia Hamdard (Hamdard University), New Delhi, India and Gulf Diagnostic Hospital Centre, Abu Dhabi, UAE
2Department of Statistics, Faculty of Economics and Management, Czech University of Life Sciences Prague, Prague, Czech Republic
3Department of Pharmaceutics, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi, India
4Director, Sunlight, Nutrition, and Health Research Center, San Francisco, CA USA

*Corresponding Author: Afrozul Haq, Professor of Food Technology and Former Dean, School of Interdisciplinary Sciences and Technology, Department of Food Technology, School of Interdisciplinary Sciences, Jamia Hamdard (Hamdard University), New Delhi, India.

Received: August 17, 2021; Published: February 28, 2022


Background and Aims: The prevalence of vitamin D deficiency—having serum 25-hydroxyvitamin D [25(OH)D] concentrations less than 75 nmol/L (30 ng/mL)—is a common phenomenon worldwide. Regardless of age, vitamin D deficiency is a serious health problem, especially in women and children. Despite plentiful of sunshine, D-deficiency is particularly a major health problem for residents of the Middle Eastern countries. The data presented in this paper are specifically of the juvenile population living in the United Arab Emirates (UAE)—that is, residents younger than 18 years from various countries.

Methods: Vitamin D estimation was done by chemiluminescence immunoassay, a quantitative immunoassay method used to determine total 25(OH)D in serum on a fully automated analyzer. Currently, the ideal methods for measuring 25(OH)D3 are based on high-performance liquid chromatography (HPLC) or liquid chromatography with tandem mass spectrometry detection. This method simultaneously measures 25(OH)D2 and 25(OH)D3. HPLC and mass spectrometry can differentiate between those two forms, yielding distinct results for each fraction.

Results: Vitamin D deficiency was highly prevalent among children aged 13-18 years living in the United Arab Emirates. Female sex and ethnicity were the variables most commonly associated with vitamin D deficiency. An inverse relationship exists between the level of 25(OH)D and age of juveniles. Among the study groups, UAE nationals have the lowest level of 25(OH)D. The severe deficiency (<25 nmol/L) was most prevalent in UAE national teenage girls aged 13-18 years (51.0%).

Conclusion: Among the age, sex, and nationality variables, age was the most important factor associated with serum 25(OH)D levels. Levels of serum 25(OH)D were inversely correlated with age in children. Severe vitamin D deficiency [25(OH)D < 25 nmol/L] was present in 9.5% of patients aged 1-3 years, 56.4% aged 7-9 years, and 79.9% aged 13-15 years. Therefore, vitamin D deficiency increased with age.

Keywords: Vitamin D Deficiency; Children; 25(OH)D; 1,25(OH)2D; Age and Sex; UAE


  1. E K McCarthy and M Kiely. “Vitamin D and muscle strength throughout the life course: a review of epidemiological and intervention studies”. Journal of Human Nutrition and Dietetics 6 (2015): 636-645.
  2. C Braegger., et al. “Vitamin D in the healthy European paediatric population”. Journal of Pediatric Gastroenterology and Nutrition 6 (2013): 692-701.
  3. C Palacios and L Gonzalez. “Is vitamin D deficiency a major global public health problem?” Journal of Steroid Biochemistry and Molecular Biology 144 (2014): 138-145.
  4. DH Kwon., et al. “High prevalence of vitamin D deficiency in 2-17year olds presenting with acute fractures in southern Australia”. Bone Reports 5 (2016): 153-157.
  5. N Radhakishun., et al. “High prevalence of vitamin D insufficiency/deficiency in Dutch multi-ethnic obese children”. European Journal of Pediatrics 2 (2015): 183-190.
  6. D Modan-Moses., et al. “High prevalence of vitamin D deficiency and insufficiency in adolescent inpatients diagnosed with eating disorders”. International Journal of Eating Disorders6 (2015): 607-614.
  7. JS Wimalawansa. “Vitamin D in the new millennium”. Current Osteoporosis Reports10 (2012): 4-15.
  8. RI Reid. “What diseases are causally linked to vitamin D deficiency?”. Archives of Disease in Childhood 2014 (2015): 307961.
  9. EL Carson., et al. “Vitamin D, Muscle Function, and Cardiorespiratory Fitness in Adolescents From the Young Hearts Study”. The Journal of Clinical Endocrinology and Metabolism 100 (2015) 4621-4628.
  10. , et al. “Vitamin D status and childhood health”. Korean Journal of Pediatrics 56.10 (2013): 417-423.
  11. M Mäkinen., et al. “Serum 25 Hydroxyvitamin D Concentrations in Children Progressing to Autoimmunity and Clinical Type 1 Diabetes”. The Journal of Clinical Endocrinology and Metabolism2 (2016): 723-729.
  12. B Dawson-Hughes., et al. “Intratrial Exposure to Vitamin D and New-Onset Diabetes Among Adults With Prediabetes: A Secondary Analysis From the Vitamin D and Type 2 Diabetes (D2d) Study”. Diabetes Care 12 (2020): 2916-2922.
  13. A R Martineau., et al. “Vitamin D supplementation to prevent acute respiratory infections: individual participant data meta-analysis”. Health Technology Assess2 (2019): 1-44.
  14. A Lis-Święty., et al. “Facts and speculations on vitamin d in prevention and treatment of atopic dermatitis”. Wiadomości Lekarskie (Warsaw, Poland: 1960) 8 (2019): 1527-1531.
  15. SR Hattangdi-Haridas., et al. “Vitamin D Deficiency and Effects of Vitamin D Supplementation on Disease Severity in Patients with Atopic Dermatitis: A Systematic Review and Meta-Analysis in Adults and Children”. Nutrients 8 (2019): 1854.
  16. RD Lewis and EM Laing. “Conflicting reports on vitamin D supplementation: evidence from randomized controlled trials”. Molecular and Cellular Endocrinology 410 (2015): 11-18.
  17. JL Josefson., et al. “Maternal BMI Associations with Maternal and Cord Blood Vitamin D Levels in a North American Subset of Hyperglycemia and Adverse Pregnancy Outcome (HAPO) Study Participants”. PLoS ONE3 (2016): 0150221.
  18. N Dressler., et al. “BMI and season are associated with vitamin D deficiency in women with impaired fertility: a two-centre analysis”. Archives of Gynecology and Obstetrics4 (2016): 907-914.
  19. A Haq., et al. “Vitamin D deficiency: A single centre analysis of patients from 136 countries”. Journal of Steroid Biochemistry and Molecular Biology 164 (2016): 209-213.
  20. A Haq., et al. “Vitamin D status among the juvenile population: A retrospective study”. Journal of Steroid Biochemistry and Molecular Biology 175 (2018): 49-54.
  21. S Abbas., et al. “Dietary vitamin D and calcium intake and premenopausal breast cancer risk in a German case-control study”. Nutrition Cancer1 (2007): 54-61.
  22. Z Cai., et al. “Determination of serum 25-hydroxyvitamin D status among population in southern China by a high accuracy LC-MS/MS method traced to reference measurement procedure”. Nutrition and Metabolism 17 (2020): 8.
  23. W L Arneson and D L Arneson. “Current methods for routine clinical laboratory testing of vitamin D levels”. Lab Medicine Winter 44 (2013): e38-e42.
  24. L Abdel-Wareth., et al. “Total vitamin D assay comparison of the Roche diagnostics vitamin D total electrochemiluminescence protein binding assay withthe chromsystems HPLC method in a population with both D2 and D3 forms of vitamin D”. Nutrients 5 (2013): 971-980.
  25. A Heijboer., et al. “Accuracy of 6 Routine 25-Hydroxyvitamin D assays: Influence of vitamin D binding protein concentration”. Clinical Chemistry 58 (2012): 543-548.
  26. F J Brayan. “Manly Multivariate Statistical Methods: A primer, Third edition”. CRC Press (2004).
  27. ML Rokach Oded. “Data mining with decision trees: theory and applications”. World Scientific. World Scientific Publishing, Hardcover (2014).
  28. DY Lee., et al. “Relationship between serum 25-hydroxyvitamin D concentration and risks of metabolic syndrome in children and adolescents from Korean National Health and Nutrition Examination survey 2008-2010”. Annals of Pediatric Endocrinology and Metabolism 20 (2015): 46-52.
  29. IH Chung., et al. “Vitamin D deficiency in Korean children: prevalence, risk factors, and the relationship with parathyroid hormone levels”. Annals of Pediatric Endocrinology and Metabolism 19 (2014): 86-90.
  30. G Karagüzel., et al. “Seasonal Vitamin D Status of Healthy Schoolchildren and Predictors of Low Vitamin D Status”. Journal of Pediatric Gastroenterology and Nutrition 58 (2014): 654-660.
  31. I Öhlund., et al. “Serum 25-hydroxyvitamin D levels in preschool-age children in northern Sweden are inadequate after summer and diminish further during winter”. Journal of Pediatric Gastroenterology and Nutrition 56 (2013): 551-555.
  32. CM Gordon., et al. “Prevalence of vitamin D deficiency among healthy adolescents”. Archives of Pediatrics and Adolescent Medicine 58 (2004): 531-537.
  33. J Guillemant., et al. “Vitamin D status during puberty in French healthy male adolescents”. Osteoporosis International 10 (1999): 222-225.
  34. FL Weng., et al. “Risk factors for low serum 25-hydroxyvitamin D concentrations in otherwise healthy children and adolescents”. The American Journal of Clinical Nutrition 86 (2007): 150-158.
  35. LH Foo., et al. “Relationship between vitamin D status, body composition and physical exercise of adolescent girls in Beijing”. Osteoporosis International 20 (2009): 417-425.
  36. Pludowski P., et al. Journal of Steroid Biochemistry and Molecular Biology 175 (2018): 125-135.


Citation: Afrozul Haq., et al. “A Retrospective Study of 25(OH)D Levels in Children of Different Age, Sex, and Nationality". Acta Scientific Nutritional Health 6.3 (2022): 115-124.


Copyright: © 2022 Afrozul Haq., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


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