Ultrasound-assisted Extraction and Characterization of Mexican Moringa oleifera Seed Oil: Identification of Tocopherols and Phytosterols Using UPLC-APCI+MS/MS
Ivet Gallegos-Marín1, María L Juárez-Rafael2, Blanca D Vázquez-Cabral3, Erasmo Herman-Lara2, Betsabé Hernández-Santos2, Jesús Rodríguez-Miranda2, Alberto Álvarez-Castillo4, Susana Santoyo5 and Cecilia E Martínez-Sánchez2*
1Conacyt-Tecnológico Nacional De México/Instituto Tecnológico De Tuxtepec, Calz, Dr. Víctor Bravo Ahuja Col. Predio el Paraíso, Tuxtepec, Oax, México
2Tecnológico Nacional De México/Instituto Tecnológico De Tuxtepec, Av. Dr. Víctor Bravo Ahuja s/n, De Mayo, Tuxtepec, Oax, México
3Tecnológico Nacional De México/Instituto Tecnológico De Durango, Department De Ing, Química y Bioquímica, Blvd, Felipe Pescador, Durango, Dgo, México
4Tecnológico Nacional De México/Instituto Tecnológico De Zacatepec, Tecnológico, Plan De Ayala, Zacatepec De Hidalgo, México
5Instituto De Investigación En Ciencias De la Alimentación CIAL (CSIC-UAM), CEI UAM + CSIC, C/Nicolás Cabrera 9, Universidad Autónoma De Madrid, Madrid, Spain
*Corresponding Author: Cecilia Eugenia Martínez Sánchez, Tecnológico Nacional De México/Instituto Tecnológico De Tuxtepec, Av. Dr. Víctor Bravo Ahuja s/n, De Mayo, Tuxtepec, Oax, México.
November 18, 2021; Published: December 15, 2021
Ultrasound-assisted extraction (UAE) from Mexican Moringa oleifera seed oil, characterization, and identification of tocopherols and phytosterols using UPLC-APCI+MS/MS were investigated. The extraction process variables such as sample/solvent ratio (6 - 20 g/mL), temperature (40 - 60°C) and ultrasound time (10 - 20 min) were evaluated on yield, extraction efficiency, peroxide index, p-anisidine, Totox value and free fatty acids. Optimization was carried out by Response Surface Methodology (RSM). The three optimal treatments (A, B and C) were determined by tocopherols and phytosterols content using UPLC-APCI+MS/MS. Antioxidant capacity was also evaluated. Alpha-tocopherol was found in a greater proportion in all three treatments and β-sitosterol was the predominant phytosterol in A and B. Treatment A presented a greater antioxidant capacity. Optimal conditions for UAE were sample/solvent ratio of 1:17.16 (w/v), 50.99 °C and extraction time of 25.95 min.
Keywords: Moringa Oleifera; Seed Oils; Tocopherols; Phytosterols; Ultrasound Assisted Extraction
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