Ivet Gallegos-Marín¹, María L Juárez-Rafael², Blanca D Vázquez-Cabral³, Erasmo Herman-Lara², Betsabé Hernández-Santos², Jesús Rodríguez-Miranda², Alberto Álvarez-Castillo⁴, Susana Santoyo⁵ and Cecilia E Martínez-Sánchez²*

¹Conacyt-Tecnológico Nacional De México/Instituto Tecnológico De Tuxtepec, Calz, Dr. Víctor Bravo Ahuja Col. Predio el Paraíso, Tuxtepec, Oax, México
²Tecnológico Nacional De México/Instituto Tecnológico De Tuxtepec, Av. Dr. Víctor Bravo Ahuja s/n, De Mayo, Tuxtepec, Oax, México
³Tecnológico Nacional De México/Instituto Tecnológico De Durango, Department De Ing, Química y Bioquímica, Blvd, Felipe Pescador, Durango, Dgo, México
⁴Tecnológico Nacional De México/Instituto Tecnológico De Zacatepec, Tecnológico, Plan De Ayala, Zacatepec De Hidalgo, México
⁵Instituto De Investigación En Ciencias De la Alimentación CIAL (CSIC-UAM), CEI UAM + CSIC, C/Nicolás Cabrera 9, Universidad Autónoma De Madrid, Madrid, Spain

*Corresponding Author: Cecilia Eugenia Martínez Sánchez, Tecnológico Nacional De México/Instituto Tecnológico De Tuxtepec, Av. Dr. Víctor Bravo Ahuja s/n, De Mayo, Tuxtepec, Oax, México.

Received: November 18, 2021; Published: December 15, 2021

Abstract

Ultrasound-assisted extraction (UAE) from Mexican Moringa oleifera seed oil, characterization, and identification of tocopherols and phytosterols using UPLC-APCI+MS/MS were investigated. The extraction process variables such as sample/solvent ratio (6 - 20 g/mL), temperature (40 - 60°C) and ultrasound time (10 - 20 min) were evaluated on yield, extraction efficiency, peroxide index, p-anisidine, Totox value and free fatty acids. Optimization was carried out by Response Surface Methodology (RSM). The three optimal treatments (A, B and C) were determined by tocopherols and phytosterols content using UPLC-APCI+MS/MS. Antioxidant capacity was also evaluated. Alpha-tocopherol was found in a greater proportion in all three treatments and β-sitosterol was the predominant phytosterol in A and B. Treatment A presented a greater antioxidant capacity. Optimal conditions for UAE were sample/solvent ratio of 1:17.16 (w/v), 50.99 °C and extraction time of 25.95 min.

Keywords: Moringa Oleifera; Seed Oils; Tocopherols; Phytosterols; Ultrasound Assisted Extraction

References

1. El Baky., et al. “Characterization of Egyptian Moringa peregrina seed oil and its bioactivities”. International Journal of Management and Business Research 2 (2013): 98-108.
2. Ojiako EN and Okeke CC. “Determination of antioxidant of Moringa oleifera seed oil and its use in the production of a body cream”. Asian Journal of Plant Science and Research 3 (2013): 1-4.
3. Yusoff MM., et al. “Aqueous enzymatic extraction of Moringa oleifera oil”. Food Chemistry 211 (2016): 400-408.
4. Brilhante RSN., et al. “Research advances on the multiple uses of Moringa oleifera: A sustainable alternative for socially neglected population”. Asian Pacific Journal of Tropical Medicine 10 (2017): 621-630.
5. Bhutada PR., et al. “Solvent assisted extraction of oil from Moringa oleifera Seeds”. Industrial Crops Production 82 (2016): 74-80.
6. Latif S and Farooq A. “Aqueous enzymatic sesame oil and protein extraction”. Food Chemistry 125 (2011): 679-684.
7. Ruttarattanamongkol K., et al. “Pilot-scale supercritical carbon dioxide extraction, physico-chemical properties and profile characterization of Moringa oleifera seed oil in comparison with conventional extraction methods”. Industrial Crops Production 58 (2014): 68-77.
8. Farooq A and Bhanger MI. “Analytical characterization of Moringa oleifera seed oil grown in temperate regions of Pakistan”. Journal of Agricultural and Food Chemistry 51 (2003): 6558-6563.
9. Mani S., et al. “Optimization of solvent extraction of Moringa (Moringa oleifera) seed kernel oil using response surface methodology”. Food and Bioproducts Processing 85 (2007): 328-335.
10. Rocchetti G., et al. “Impact of conventional/non-conventional extraction methods on the untargeted phenolic profile of Moringa oleifera leaves”. Food Research International 115 (2019): 319-327.
11. Zhong J., et al. “The application of ultrasound and microwave to increase oil extraction from Moringa oleifera seeds”. Industrial Crops and Products 120 (2018): 1-10.
12. 1999. Official Methods and Recommended Practices of the American Oil Chemists Society. Am Oil Chem Soc, 6th Edn. AOCS Press, Urbana, Illinois USA Hu B., et al. “Method for extracting oil from cherry seed by ultrasonic-microwave assisted aqueous enzymatic process and evaluation of its quality”. Journal of Chromatography A 1587 (2019): 50-60.
13. Zarrouk W., et al. “multi-component analysis (sterols, tocopherols and triterpenic dialcohols) of the unsaponifiable fraction of vegetable oils by liquid chromatography-atmospheric pressure chemical ionization-ion trap mass spectrometry”. Talanta 80 (2010): 924-934.
14. Ramadan MF. “Healthy blends of high linoleic sunflower oil with selected cold pressed oils: Functionality, stability and antioxidative characteristics”. Industrial Crops Production 43 (2013): 65-72.
15. García-Villalba R., et al. “Characterization and quantification of phenolic compounds of extra-virgin olive oils with anticancer properties by a rapid and resolutive LC-ESI-TOF MS method”. Journal of Pharmaceutical Biomedical 51 (2010): 416-429.
16. Senrayan J and Venkatachalam S. “Optimization of ultrasound-assisted solvent extraction (UASE) based on oil yield, antioxidant activity and evaluation of fatty acid composition and thermal stability of Coriandrum sativum seed oil”. Food Science Biotechnology 28 (2019): 377-386.
17. Sicaire AG., et al. “Ultrasound induced green solvent extraction of oil from oleaginous seeds”. Ultrasonics Sonochemistry 31 (2016): 319-329.
18. Zhang W., et al. “Optimized ultrasonic‐assisted extraction of papaya seed oil from Hainan/Eksotika variety”. Food Science Nutrition 7 (2019): 2692-2701.
19. Samaram S., et al. “Optimisation of ultrasound-assisted extraction of oil from papaya seed by response surface methodology: Oil recovery, radical scavenging antioxidant activity, and oxidation stability”. Food Chemistry 172 (2015): 7-17.
20. Ansorena D., et al. “Effect of fish and oil nature on frying process and nutritional product quality”. Journal of Food Science 75 (2010): 62-67.
21. Yolmeh M., et al. “Optimization of ultrasound-assisted extraction of natural pigment from annatto seeds by response surface methodology (RSM)”. Food chemistry 155 (2014): 319-324.
22. Martins PF., et al. “Techno-economic optimization of the subcritical fluid extraction of oil from Moringa oleifera seeds and subsequent production of a purified sterols fraction”. The Journal of Supercritical Fluids 107 (2016): 682-689.
23. Ogbunugafor HA., et al. “Physico-chemical and antioxidant properties of Moringa oleifera seed oil”. Pakistan Journal of Nutrition 10 (2011): 409-414.
24. Beleggia R., et al. “Effect of genotype, environment and genotype-by-environment interaction on metabolite profiling in durum wheat (Triticum durum Desf.) grain”. The Journal of Cereal Science 57 (2013): 183-192.
25. Piironen V., et al. “Plant sterols: biosynthesis, biological function and their importance to human nutrition”. Journal of the Science of Food and Agriculture 80 (2000): 939-966.
26. Leone A., et al. “Moringa oleifera seeds and oil: Characteristics and uses for human health”. International Journal of Molecular Sciences 17 (2016): 2141.
27. Hendriks HFJ., et al. “Safety long-term consumption of plant sterol esters-enriched spread”. European Journal of Clinical Nutrition 57 (2003): 681-692.
28. Uddin S., et al. “Phytosterols and their extraction from various plant matrices using supercritical carbon dioxide: a review”. Journal of the Science of Food and Agriculture 95 (2014): 1385-1394.
29. Gopalakrishnan L., et al. “Moringa oleifera: A review on nutritive importance and its medicinal application”. Food Science and Human Wellness 5 (2016): 49-56.

Citation

Citation: Cecilia E Martínez-Sánchez., et al. “Ultrasound-assisted Extraction and Characterization of Mexican Moringa oleifera Seed Oil: Identification of Tocopherols and Phytosterols Using UPLC-APCI+MS/MS". Acta Scientific Nutritional Health 6.1 (2022): 28-38.

Copyright

Copyright: © 2022 Cecilia E Martínez-Sánchez., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.