Samuel Ferreira Gonçalves¹, Sarah Hwa In Lee¹, Lara Aguiar Borges², Marta Liliane de Vasconcelos¹, Carlos Humberto Corassin¹ and Carlos Augusto Fernandes de Oliveira¹*

¹Departamento de Engenharia de Alimentos, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga, SP, Brazil
²Departamento de Tecnologia de Alimentos, Faculdade de Engenharia de Alimentos, Universidade Estadual de Campinas, Campinas, SP, Brazil

*Corresponding Author: Carlos Augusto Fernandes de Oliveira, 1Departamento de Engenharia de Alimentos, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga, SP, Brazil.

Received: September 14, 2021Published: September 24, 2021

Abstract

  This work aimed at evaluating the effect of alpha-amylase (AA, 100 mg/mL), sodium hypochlorite (SH, 0.5%), peracetic acid (PAA, 0.3%), and enzyme-biocide combinations (SH or PAA and AA) on mono-species biofilms formed by four Staphylococcus aureus strains (P01F2T1, P01F5T2, P16 and P24) previously isolated from dairy farms in São Paulo state, Brazil. Biofilm formation index (BFI) and culturable cell counts were evaluated for biofilms formed on polystyrene microplates for 72 h at 25ºC. The BFI of isolates P16 and P24 significantly decreased (P < 0.05) after treatment with PAA or SH combined with AA, compared with the biocides alone. The biofilms formed by isolates P01F2T1 and P01F5T2 had lower BFI values (P < 0.05) after treatment with combinations of PAA + AA and SH + AA, respectively. However, significant reductions in the biofilm culturable counts were observed only for isolates P01F2T1 treated with SH + AA and PAA + AA, P01F5T2 treated with SH + AA, and P24 treated with PAA + AA. Further studies are required to define the best combinations of AA and SH or PAA to completely remove S. aureus biofilms formed on plastic surfaces in processing dairy environments.

Keywords: S. aureus; Bacterial Adhesion; Peracetic Acid; Sodium Hypochlorite; Alpha-amylase

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Citation

Citation: Carlos Augusto Fernandes de Oliveira., et al. “Individual and Combined Effects of Alpha-amylase and Biocides on Biofilms Formed by Staphylococcus aureus Strains Isolated from Brazilian Dairy Farms". Acta Scientific Nutritional Health 5.10 (2021): 88-94.