Acta Scientific Nutritional Health (ASNH)(ISSN: 2582-1423)

Research Article Volume 4 Issue 12

Effects of High Protein/Low Carbohydrate Rationed Formulated Diet on Glycemic Tolerance and Control, Body Weight and Organ Histomorphometry in Experimental Diabetic Rats

Magnus Michael Chukwudike Anyakudo1* and David Opeyemi Adeniji2

1Endocrinology, Metabolism and Clinical Nutrition Research Unit of Department of Medical Physiology, Faculty of Basic Medical Sciences, University of Medical Sciences, Ondo City, Ondo State, Nigeria
2Department of Medicine, Bowen University Teaching Hospital, College of Health Sciences, Ogbomoso, Oyo State, Nigeria

*Corresponding Author: Magnus Michael Chukwudike Anyakudo, Endocrinology, Metabolism and Clinical Nutrition Research Unit of Department of Medical Physiology, Faculty of Basic Medical Sciences, University of Medical Sciences, Ondo City, Ondo State, Nigeria.

Received: November 09, 2020; Published: November 30, 2020



  Metabolic response to carbohydrate and protein ingestion plays an important role in health and disease states. This experimentally-controlled designed nutritional study aimed to determine the effects of a high protein/low carbohydrate rationed formulated (HP/LC) diet on body weight, organs (kidneys, liver, heart, lungs, spleen and testes) histomorphometry, glycemic tolerance and control in experimental diabetic and healthy rats. Twenty-four male Wistar rats randomly categorized into four groups (n = 6, each): Healthy control group (HC), Healthy treated group (HT), Diabetic control group (DC) and Diabetic treated group (DT) were used for this study which lasted eight weeks. The animals were fed according to the experimental design with water ad libitum. Diabetes was inducted with freshly prepared alloxan monohydrate solution (150 mg/kg bwt, intraperitoneally). Body weights and fasting blood sugar concentrations were measured twice weekly while oral glucose tolerance test was conducted on the last day of the study after which, the organs were extracted for weight assessment and histomorphometric analysis. In this study, the high-protein/low-carbohydrate rationed formulated diet caused significant reduction in mean body weight gain both in treated diabetic (DT: 22.6%; P = .001) and healthy (HT: 5.8%; P = .007) rats compared with their respective controls which recorded significant (P < .05) increase in body weight gain (DC: 12.4%; HC: 11.2%). In DT and HT rats, glycemic tolerance and control improved significantly (DT > HT) while no visible lesions or distortion in organs histoarchitecture observed. In conclusion, high-protein/low-carbohydrate rationed formulated diet reduced body weight gain with improved glycemic tolerance and control without pathologic consequences on organs histoarchitecture in experimental diabetic and healthy rats.

Keywords: Body Weight; Experimental Rats; Formulated-diet; Glycemic Control; Histomorphometry



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Citation: Magnus Michael Chukwudike Anyakudo and David Opeyemi Adeniji. “Effects of High Protein/Low Carbohydrate Rationed Formulated Diet on Glycemic Tolerance and Control, Body Weight and Organ Histomorphometry in Experimental Diabetic Rats".Acta Scientific Nutritional Health 4.12 (2020): 80-88.


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