Abstract

  Metabolic response to carbohydrate and protein ingestion plays an important role in health and disease states. This experimentally-controlled designed nutritional study aimed to determine the effects of a high protein/low carbohydrate rationed formulated (HP/LC) diet on body weight, organs (kidneys, liver, heart, lungs, spleen and testes) histomorphometry, glycemic tolerance and control in experimental diabetic and healthy rats. Twenty-four male Wistar rats randomly categorized into four groups (n = 6, each): Healthy control group (HC), Healthy treated group (HT), Diabetic control group (DC) and Diabetic treated group (DT) were used for this study which lasted eight weeks. The animals were fed according to the experimental design with water ad libitum. Diabetes was inducted with freshly prepared alloxan monohydrate solution (150 mg/kg bwt, intraperitoneally). Body weights and fasting blood sugar concentrations were measured twice weekly while oral glucose tolerance test was conducted on the last day of the study after which, the organs were extracted for weight assessment and histomorphometric analysis. In this study, the high-protein/low-carbohydrate rationed formulated diet caused significant reduction in mean body weight gain both in treated diabetic (DT: 22.6%; P = .001) and healthy (HT: 5.8%; P = .007) rats compared with their respective controls which recorded significant (P < .05) increase in body weight gain (DC: 12.4%; HC: 11.2%). In DT and HT rats, glycemic tolerance and control improved significantly (DT > HT) while no visible lesions or distortion in organs histoarchitecture observed. In conclusion, high-protein/low-carbohydrate rationed formulated diet reduced body weight gain with improved glycemic tolerance and control without pathologic consequences on organs histoarchitecture in experimental diabetic and healthy rats.

Keywords: Body Weight; Experimental Rats; Formulated-diet; Glycemic Control; Histomorphometry

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Citation

Citation: Magnus Michael Chukwudike Anyakudo and David Opeyemi Adeniji. “Effects of High Protein/Low Carbohydrate Rationed Formulated Diet on Glycemic Tolerance and Control, Body Weight and Organ Histomorphometry in Experimental Diabetic Rats".Acta Scientific Nutritional Health 4.12 (2020): 80-88.

Copyright

Copyright: © 2020 Magnus Michael Chukwudike Anyakudo and David Opeyemi Adeniji. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.