Acta Scientific Nutritional Health (ASNH)(ISSN: 2582-1423)

Research Article Volume 4 Issue 11

Bromelain and Amylase Assisted Extraction of Cucurbita pepo Seed Oil Enriched with Phytosterol

Nguyen Thi Minh Tu1*, Kieu Thi Hoang Yen1, Tran Huong Nga1, Le Tat Thanh2 and Dang Thi Thu2

1School of Biotechnology and Food Technology, Hanoi University of Science and Technology, Vietnam
2Institute of Natural Products Chemistry, Vietnam Academic of Science and Technology, Vietnam

*Corresponding Author: Nguyen Thi Minh Tu, School of Biotechnology and Food Technology, Hanoi University of Science and Technology, Vietnam.

Received: September 22, 2020; Published: October 28, 2020



  Pumpkin (Cucurbita pepo) seeds’ hydrolysis condition was investigated in order to achieve oil containing high content of phytosterol by using enzyme bromelain and amylase. For this purpose, hydrolysis conditions of bromelain and amylase including ratio of C. pepo to water, ratio of concentration of the enzyme bromelain/amylase to substance, temperature, pH, hydrolysis time were investigated.

Bromelain hydrolysis of C. pepo seeds with 1.75% enzyme at 55oC for 7hrs at pH of 7 gave 27.83% oil and 1909.95 mg phytosterol in 100 g oil. For amylase hydrolysis these figures were 0.9%; 50oC; 5.5 hrs and 7, respectively; and the oil yield was 14.02% with 2245.03 mg phytosterol in 100 g oil.

Although oil yield by using enzyme bromelain (27.83%) and enzyme amylase (14.02%) was lower than that by using solvent and ultrasonic methods (26.57% to 42.74%), phytosterol content inversely was as 2 to 3 times as higher (631.41 - 772.78 to 19909.95 - 2245.03 mg/100g oil). Fourteen compounds were found in phytosterol mixture obtained by amylase hydrolysis, among them three important e.g. campesterol, stigmasterol, β - sitosterol accounted for 315.460, 120.111 and 189.987 mg/100g oil respectively.


Keywords: Amylase; Bromelain; Oil of Pumpkin Seed; Phytosterol



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Citation: Nguyen Thi Minh Tu., et al. “Bromelain and Amylase Assisted Extraction of Cucurbita pepo Seed Oil Enriched with Phytosterol".Acta Scientific Nutritional Health 4.11 (2020): 50-56.


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