Acta Scientific Nutritional Health (ASNH)(ISSN: 2582-1423)

Research Article Volume 4 Issue 11

Preparation and Quality Evaluation of Yogurt by Incorporation with Moringa oleifera Leaves Powder

Sanjaya Karki*, Saugat Prajapati and Susma Bhattarai

College of Applied Food and Dairy Technology, Purbanchal University, Kathmandu, Nepal

*Corresponding Author: Sanjaya Karki, College of Applied Food and Dairy Technology, Purbanchal University, Kathmandu, Nepal.

Received: July 27, 2020; Published: October 07, 2020



  Moringa oleifera leaves is fill with a pack of nutrients therefore, this study was carried out in order to enrich the nutritional value of traditional yoghurt. The powdered leaves of Moringa oleifera were analyzed for moisture content, vitamin C, protein, ash and fat content and the result was found to be 7.34 ± 0.04%, 17.24 ± 0.10 mg/100g, 25.89 ± 1.42%, 9.67 ± 0.40% and 2.1 ± 0.2% respectively and mesh size was 140 µm. From the sensory analysis the product having 0.5% Moringa oleifera leaves powder incorporated yoghurt was chosen for further analysis as it showed comparable result with control yoghurt. The chemical analysis of moringa incorporated yoghurt showed that fat, lactose, protein, total solid, acidity, calcium and pH were 2.9 ± 0.11%, 3.71 ± 0.02, 4.51 ± 0.09%, 22.18 ± 0.19%, 0.82 ± 0.02, 356.83 ± 10.19 (mg/100g) and 4.3 ± 0.02 whereas that of control yoghurt were found to be 2.76 ± 0.07%, 3.86 ± 0.03, 3.7 ± 0.4%, 19.90 ± 0.28%, 0.69 ± 0.01, 244.67 ± 10.19 (mg/100g) and 4.5 ± 0.01 respectively.

  The fresh yoghurt was found to contain no any coliform, yeast and mold count. Setting time and syneresis for moringa leaves incorporated yoghurt (0.5%) i.e. sample A was found 270 minutes and 34.05 ± 1.30%, respectively whereas setting time for control sample i.e. Sample E was 300 minutes and syneresis was 22.43 ± 2.12%. Acidity was continuously observed in the interval of 30 minutes till the yoghurt sets which showed rapid increase in acidity in Moringa oleifera incorporated yoghurt than that of control yoghurt. Acidity rose from 0.69 to 1.14 in control yoghurt and for Moringa oleifera incorporated yoghurt it rose from 0.82 to 1.40 during 12 days storage period. The total plate count of the yoghurt decreased during the storage period of 12 days from 1.20 × 109 cfu/ml to 1.1 × 103 cfu/ml for Sample A and from 1.76 × 109 cfu/ml to 4.4 × 103 cfu/ml for Sample E i.e. control yoghurt.

Keywords: Moringa oleifera; Syneresis; Incorporated Yoghurt; Chemical Analysis; Sensory Analysis



  1. Ahmed S. “Studies on the manufacture of flavored yoghurt drinks from skim milk”. MS Thesis, Department of Dairy Science, Bangladesh Agricultural University, Mymensingh (2004).
  2. “Official Method of Analysis”, 18th ed. Association of Official Analytical Chemist, Washington D.C (2005).
  3. Caceres A., et al. “Pharmacological properties of Moringa oleifera. 1: Preliminary screening for antimicrobial activity”. Journal of Ethnopharmacology 33 (1991): 213-216.
  4. Codex Alimentarius Commission (CAC). "General Standard for the Use of Dairy Terms 206-1999".
  5. Yogurt. 21 CFR 131.200, Code of Federal Regulations. U. S. Dept. of Health and Human Services, Washington, DC (2013).
  6. Foidl N., et al. “The potential of Moringa oleifera”.
  7. Fuglie LJ. “The Miracle tree: Moringa oleifera: Natural Nutrition for Tropics”. Church World Service, Dakkar (1999): 68.
  8. Hassan FAM., et al. “Utilization of Moringa oleifera leaves in production of yoghurt”. International Journal of Dairy Science2 (2016): 69-74.
  9. International Dairy Federation (IDF); 1990 and 1998. Enumeration of Yeast and mold count.
  10. Kasolo JN., et al. “Phytochemicals and uses of Moringa oleifera leaves in Ugandan rural communities”. Journal of Medicinal Plants Research 4 (2010): 753-757.
  11. KC J B and Rai BK. Basic Food Analysis Handbook (1st Ed.). Prompt Printers Pvt. Ltd. Anamnagar, Kathmandu, Nepal (2007).
  12. Lamsal B. “Queen of Green”. The Himalayan times (2015).
  13. Lee WJ and Lucey JA. “Structure and physical properties of yoghurt gels”. Journal of Dairy Science 87 (2014): 3153.
  14. Makkar HPS and K Becker. “Nutrients and antiquality factors in different morphological parts of the Moringa oleifera tree”. Journal of Agricultural Science 128 (1997): 311-322.
  15. Mbaiguinam Mbailao., et al. “Proximal and Elemental Composition of Moringa oleifera (Lam) Leaves from Three Regions of Chad”. Journal of Food Resource Science 3 (2014): 12-20.
  16. Mishra SP., et al. “Processing of Moringa oleifera leaves for human consumption”. Bulletin of Environment Pharmacology and Life Science 2 (2012): 28-31.
  17. (NDDB) ND. Laboratory Handbook for Dairy Industry (2001).
  18. Peixoto JR., et al. “In vitro antibacterial effect of aqueous and ethanolic Moringa Leaf extract”. Asian Pacific Journal of Tropical Medicine 4 (2011): 201-214.
  19. Pokhrel CP., et al. “Moringa oleifera: A potential cash crop in Nepal”. International symposium on “Healthy Society and Healthy World” (2016).
  20. Putaya H A N., et al. “Proximate Composition and Phytochemical screening of Moringa oleifera Leaves”. Applied Research Journal9 (2015): 470-472.
  21. Omotesho K F., et al. “The potential of moringa tree for poverty alleviation and rural development: Review of evidences on usage and efficacy”. International Journal of Development and Sustainability 2 (2013): 799-813.
  22. Rayamajhi S. Preparation and quality evaluation of pineapple juice incorporated yoghurt, Dissertation, B. Tech. (Food), Central Campus of Technology, Tribhuvan University, Nepal (2011).
  23. Salji T P and Ismail AA. “Effect of initial acidity of plain yoghurt on acidity changes during refrigerated storage”. Journals of Food Science1 (1983): 258-259.
  24. Sangheetha S., et al. “Optimization of palmyrah (borrasus flabellifer) fruit pulp in different varieties of fruit yoghurts”. International Journal of Multidisciplinary1 (2014): 91-103.
  25. Singhal RS., et al. “A study on degradation kinetics of ascorbic acid in drumstick (Moringa olifera) leaves during cooking”. Journal of Science, Food Agriculture 85 (2005): 1953-1958.
  26. Sodamode A., et al. “Proximate analysis, mineral contents and functional properties of Moringa oleifera leaf protein concentrate”. IOSR Journal of Applied Chemistry 4 (2013): 47-51.
  27. Tree for life. The Moringa Book (2005).


Citation: Sanjaya Karki., et al. “Preparation and Quality Evaluation of Yogurt by Incorporation with Moringa oleifera Leaves Powder".Acta Scientific Nutritional Health 4.11 (2020): 02-08.


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