Abstract

Different methods of detection have been used to detect milk adulteration, but in recent years the use of nanomaterials has demonstrated to be an interesting tool in this field, because of their interesting optical properties. A gold nanoparticle (AuNP) probe strategy for testing milk authenticity was developed, which relies on the colorimetric differentiation of a particular DNA sequence, due to the differential aggregation profiles exhibited by the AuNPs in the presence or absence of specific target hybridization. Gold nanoparticles were conjugated with thiolated oligonucleotides which specifically amplify a 271 bp fragment of cow mitochondrial DNA. In the presence of a complementary target preventing aggregation of the AuNPs when acid was added, the reaction mixtures retained the original pink coloration of the colloidal particles, whereas they turned purple in the opposite event. Negative and goat reaction mixtures showed a purplish coloured solution with a peak at ≥570nm, while samples containing bovine DNA have an absorbance closer to the characteristic peak of the AuNPs at 520-525 nm. Presence of bovine milk even at traces levels was detected, achieving a level of detection comparable with PCR + Electrophoresis. The use of AuNPs for the colorimetric detection of DNA targets from undeclared species in milk products provides an inexpensive and easy-toperform alternative to common molecular assays. However, the tested oligonucleotides only proved to be effective under very concrete conditions, due to their low specificity. The technology described here can be further developed and more specific oligonucleotides are advised to be tested. Even though, this method offers the possibility to accommodate for detection of many cases of adulteration and fraudulent practices in different food matrices.

Keywords: Gold Nanoparticles; Food Authentication; Goat Milk Adulteration; Detection Limit

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Citation

Citation: Jose Manuel Llopis Ortiz., et al. “Effectual Gold Nanoprobe Sensor for Screening Cow Milk Adulteration in Goat Milk".Acta Scientific Nutritional Health 4.10 (2020): 37-43.

Copyright

Copyright: © 2020 Jose Manuel Llopis Ortiz et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.