Na Ryung Choi1, Hyeon Tae Kang2, Seung Min Lee2, Yeon-Lim Suh3, Hyukjin Kwon4, Jaekyu Noh4, Sang Bong Ahn5 and Dae Won Jun1,2*
1Department of Internal Medicine, Hanyang University School of Medicine, Seoul, Republic of Korea
2Department of Translational Medicine Hanyang University Graduate School of Biomedical Science and Engineering, Seoul, Republic of Korea
3Department of Pathology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea
4NI&Pharm INC, Seoul, Republic of Korea
5Department of Internal Medicine, Eulji University School of Medicine, Seoul, Republic of Korea
*Corresponding Author: Dae Won Jun, Department of Internal Medicine, Hanyang University College of Medicine, Republic of Korea.
Received: July 20, 2020; Published: August 24, 2020
Sericin is a component of cocoons. Studies have shown that sericin improves metabolic disease in animal model. However, it is not yet known whether sericin is effective in the treatment of non-alcoholic steatohepatitis. Twenty C57BL/6N mice were divided into control and sericin treated group. High fat diet was fed to mice for 10 weeks. From 11th to 21st week sericin and distilled water were administered assigned groups via oral gavage. Body weight and liver to body weight ratio was assessed. AST, ALT, triglyceride, cholesterol and blood glucose levels were measured. qRT-PCR, Electro-microscopy, and Free fatty acid induced lipotoxicity assay were performed. Mitochondrial membrane potential was checked. As a result, there were no differences in body weight and diet intake in two groups. However, liver weight and liver/body weight ratio were significantly decreased in sericin group than in control group. Both the degree of hepatic steatosis and inflammation were lower in sericin group than the control group. NAFLD activity score were also decreased in treatment group. Serum ALT, AST, and triglyceride levels as well as area under receiver operating characteristics of oral glucose tolerance test were significantly decreased in sericin group. Hepatic mRNA expressions of TNF-α, MCP-1, and IL-6 mRNA were decreased in sericin group. Electron microscopy findings showed restoration of abnormal mitochondria with sericin treatment. Lipotoxicity induced HepG2 cell death was significantly attenuated in palmitic acid and sericin co-treated cells. Sericin also prevented palmitic acid induced mitochondrial depolarization. In conclusion, Sericin peptide treatment attenuated non-alcoholic steatohepatitis via mitochondrial function modulation.
Keywords: Sericin; Nonalcoholic steatohepatitis; Mitochondria
Citation: Dae Won Jun.,et al. “Sericin Peptide Attenuates Non-Alcoholic Steatohepatitis Via Mitochondrial Function Modulation". Acta Scientific Nutritional Health 4.9 (2020): 42-52.
Copyright: © 2020 Dae Won Jun.,et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.