Acta Scientific Nutritional Health (ASNH)(ISSN: 2582-1423)

Research Article Volume 4 Issue 9

Sericin Peptide Attenuates Non-Alcoholic Steatohepatitis Via Mitochondrial Function Modulation

Na Ryung Choi1, Hyeon Tae Kang2, Seung Min Lee2, Yeon-Lim Suh3, Hyukjin Kwon4, Jaekyu Noh4, Sang Bong Ahn5 and Dae Won Jun1,2*

1Department of Internal Medicine, Hanyang University School of Medicine, Seoul, Republic of Korea
2Department of Translational Medicine Hanyang University Graduate School of Biomedical Science and Engineering, Seoul, Republic of Korea
3Department of Pathology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea
4NI&Pharm INC, Seoul, Republic of Korea
5Department of Internal Medicine, Eulji University School of Medicine, Seoul, Republic of Korea

*Corresponding Author: Dae Won Jun, Department of Internal Medicine, Hanyang University College of Medicine, Republic of Korea.

Received: July 20, 2020; Published: August 24, 2020

×

Abstract

Sericin is a component of cocoons. Studies have shown that sericin improves metabolic disease in animal model. However, it is not yet known whether sericin is effective in the treatment of non-alcoholic steatohepatitis. Twenty C57BL/6N mice were divided into control and sericin treated group. High fat diet was fed to mice for 10 weeks. From 11th to 21st week sericin and distilled water were administered assigned groups via oral gavage. Body weight and liver to body weight ratio was assessed. AST, ALT, triglyceride, cholesterol and blood glucose levels were measured. qRT-PCR, Electro-microscopy, and Free fatty acid induced lipotoxicity assay were performed. Mitochondrial membrane potential was checked. As a result, there were no differences in body weight and diet intake in two groups. However, liver weight and liver/body weight ratio were significantly decreased in sericin group than in control group. Both the degree of hepatic steatosis and inflammation were lower in sericin group than the control group. NAFLD activity score were also decreased in treatment group. Serum ALT, AST, and triglyceride levels as well as area under receiver operating characteristics of oral glucose tolerance test were significantly decreased in sericin group. Hepatic mRNA expressions of TNF-α, MCP-1, and IL-6 mRNA were decreased in sericin group. Electron microscopy findings showed restoration of abnormal mitochondria with sericin treatment. Lipotoxicity induced HepG2 cell death was significantly attenuated in palmitic acid and sericin co-treated cells. Sericin also prevented palmitic acid induced mitochondrial depolarization. In conclusion, Sericin peptide treatment attenuated non-alcoholic steatohepatitis via mitochondrial function modulation.

Keywords: Sericin; Nonalcoholic steatohepatitis; Mitochondria

×

References

  1. Li J., et al. "Prevalence, Incidence, and Outcome of Non-Alcoholic Fatty Liver Disease in Asia, 1999-2019: A Systematic Review and Meta-Analysis”. Lancet Gastroenterology and Hepatology 4.5 (2019): 389-398.
  2. Lim CY., et al. "Effects of Carnitine on Peripheral Blood Mitochondrial DNA Copy Number and Liver Function in Non-Alcoholic Fatty Liver Disease”. Korean Journal of Gastroenterology 55.6 (2010): 384-9.
  3. Oh H., et al. "Non-Alcoholic Fatty Liver Diseases: Update on the Challenge of Diagnosis and Treatment”. Clinical and Molecular Hepatology 22.3 (2016): 327-335.
  4. Simoes ICM., et al. "Mitochondria in Non-Alcoholic Fatty Liver Disease”. The International Journal of Biochemistry and Cell Biology 95 (2018): 93-99.
  5. Nassir F and JA Ibdah. "Role of Mitochondria in Nonalcoholic Fatty Liver Disease”. International Journal of Molecular Sciences 15.5 (2014): 8713-8742.
  6. Kundu Subhas C., et al. "Natural Protective Glue Protein, Sericin Bioengineered by Silkworms: Potential for Biomedical and Biotechnological Applications”. JPPS Progress in Polymer Science 33.10 (2008): 998-1012.
  7. Padol Amol., et al. "Natural Biomaterial Silk and Silk Proteins: Applications in Tissue Repair”. International Journal of Materials and Biomaterials Applications 2 (2012): 19-24.
  8. Ampawong S., et al. “Sericin Ameliorated Dysmorphic Mitochondria in High-Cholesterol Diet/Streptozotocin Rat by Antioxidative Property”. Experimental Biology and Medicine (Maywood) 242.4 (2017): 411-421.
  9. Kleiner DE., et al. "Design and Validation of a Histological Scoring System for Nonalcoholic Fatty Liver Disease”. Hepatology 41.6 (2005): 1313-1321.
  10. Okazaki Y., et al. "Consumption of Sericin Reduces Serum Lipids, Ameliorates Glucose Tolerance and Elevates Serum Adiponectin in Rats Fed a High-Fat Diet”. Bioscience, Biotechnology, and Biochemistry 74.8 (2010): 1534-1538.
  11. Lapphanichayakool P., et al. “Hypocholesterolemic Effect of Sericin-Derived Oligopeptides in High-Cholesterol Fed Rats”. Journal of Natural Medicines 71.1 (2017): 208-215.
  12. Deori M., et al. “Antioxidant Effect of Sericin in Brain and Peripheral Tissues of Oxidative Stress Induced Hypercholesterolemic Rats”. Frontiers in Pharmacology 7 (2016): 319.
  13. Jun DW., et al. "Prevention of Free Fatty Acid-Induced Hepatic Lipotoxicity by Carnitine Via Reversal of Mitochondrial Dysfunction”. Liver International 31.9 (2011): 1315-24.
  14. Gonzalez-Franquesa A and ME Patti. "Insulin Resistance and Mitochondrial Dysfunction”. Advances in Experimental Medicine and Biology 982 (2017): 465-520.
×

Citation

Citation: Dae Won Jun.,et al. “Sericin Peptide Attenuates Non-Alcoholic Steatohepatitis Via Mitochondrial Function Modulation". Acta Scientific Nutritional Health 4.9 (2020): 42-52.




Metrics

Acceptance rate30%
Acceptance to publication20-30 days
Impact Factor1.316

Indexed In





News and Events


  • Certification for Review
    Acta Scientific certifies the Editors/reviewers for their review done towards the assigned articles of the respective journals.
  • Submission Timeline for Upcoming Issue
    The last date for submission of articles for regular Issues is July 10, 2024.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Article of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of "Best Article of the Issue"
  • Welcoming Article Submission
    Acta Scientific delightfully welcomes active researchers for submission of articles towards the upcoming issue of respective journals.

Contact US