Acta Scientific Nutritional Health (ASNH)(ISSN: 2582-1423)

Research Article Volume 4 Issue 4

Role of Next Generation Sequencing (NGS) in Searching for Promising Lactic Acid Strains Used as Animal Probiotic

Bushra Urooj1,2, Maria Sohail1, Allah Nawaz Khan2, Muhammad Aqeel1, Dur Shehwar2, Abdul Manan1 and Shakira Ghazanfar1*

11National Institute of Genomics and Agriculture Biotechnology (NIGAB), National Agriculture Research Centre, Park Road, Islamabad, Pakistan
2Department of Life Sciences, Abasyn University Islamabad campus Park road, Islamabad, Pakistan

*Corresponding Author: Shakira Ghazanfar, National Institute of Genomics and Agriculture Biotechnology (NIGAB), National Agricultural Research Centre, Park Road, Islamabad, Pakistan.

Received: February 21, 2020; Published: April 07, 2020

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  Next generation sequencing (NGS) has revolutionized the research related to the study of animal gut. Scientists point out, that little information has been investigated regarding the link between the animal health production and ruminal gut ecosystem. Hence, we review the advance progress in studying the host-microbial interactions. Scientists have developed/used new molecular approaches and computational techniques for metagenomic and whole genome sequencing analysis to search the promising probiotic strains which has the capability to improve milk yield. For the preparation of the unique probiotic product researcher should work on the isolation of microbial flora to conventional microbial molecular techniques merged with complex algorithms of computational efficiency for whole genome sequence analysis. Ruminal-gut microbiota is considered as a major source of unique probiotic bacterial strains. Probiotics are “live microorganisms which when administered in adequate amounts confer a health benefit on the host”.

Keywords: Next Generation Sequencing (NGS); Lactic Acid Bacteria (LAB); Animal Probiotic

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References

  1. Food and Agricultural Organization of the United Nations and World Health Organization. “Health and nutritional properties of probiotics in food including powder milk with live lactic acid bacteria” (2001).
  2. Hill C., et al. “The International Scientific Association for Probiotics and Prebiotics consensus statement on the scope and appropriate use of the term probiotic”. Nature Reviews Gastroenterology and Hepatology 11 (2014): 506-514.
  3. Curry A. "The milk revolution”. Nature7460 (2013): 20-22.
  4. Chaisson MJ., et al. “De novo fragment assembly with short mate-paired reads: Does the read length matter?” Genome Res 192 (2009): 336-346.
  5. Urbach E., et al. “The ldh phylogeny for environmental isolates of Lactococcus lactis is consistent with rRNA genotypes but not with phenotypes”. Appl Environ Microbiol2 (1997): 694-702.
  6. Tailliez P., et al. “Molecular Diversity and Relationship within Lactococcus lactis, as revealed by Randomly Amplified Polymorphic DNA (RAPD)”. Systematic and Applied Microbiology 4 (1998): 530-538.
  7. Holzapfel WH., et al. “Taxonomy and important features of probiotic microorganisms in food and nutrition”. The American Journal of Clinical Nutrition2 (2001): 365-373.
  8. Culligan EP., et al. “Combined metagenomic and phenomic approaches identify a novel salt tolerance gene from the human gut microbiome”. Frontiers in Microbiology (2014): 189.
  9. Lugli GA., et al. “Compositional assessment of bacterial communities in probiotic supplements by means of metagenomic techniques”. International Journal of Food Microbiology (2019): 294.
  10. Lin C., et al. “Comparison of ALK detection by FISH, IHC and NGS to predict benefit from crizotinib in advanced non-small-cell lung cancer”. Lung Cancer 131 (2019): 62-68.
  11. Coman MM., et al. “Probiotic characterization of Lactobacillus isolates from canine faeces”. Journal of Applied Microbiology4 (2019): 1245-1256.
  12. Bradley PH., et al. “Phylogeny-corrected identification of microbial gene families relevant to human gut colonization”. PLoS Computational Biology 8 (2018): e1006242.
  13. Tyagi A., et al. “Shotgun metagenomics offers novel insights into taxonomic compositions, metabolic pathways and antibiotic resistance genes in fish gut microbiome”. Achieves of Microbiology3 (2019): 295-303.
  14. Wen C., et al. “Quantitative metagenomics reveals unique gut microbiome biomarkers in ankylosing spondylitis”. Genome Biology1 (2017): 142.
  15. Dong B., et al. “High Throughput Identification of Antimicrobial Peptides from Fish Gastrointestinal Microbiota”. Toxins 9 (2017): 266.
  16. Koser CU., et al. “Routine Use of Microbial Whole Genome Sequencing in Diagnostic and Public Health Microbiology”. PLoS Pathogens 8 (2012): 8.
  17. Fraser CM., et al. “Microbial genome sequencing”. Nature 406 (2000): 799-803.
  18. Aguiar-Pulido V., et al. Evolutionary Bioinformatics (2016).
  19. Jung JY., et al. “Metatranscriptomic analysis of lactic acid bacterial gene expression during kimchi fermentation”. International Journal of Food Microbiology2-3 (2013):171-179.
  20. Gosalbes MJ., et al. “Metatranscriptomic approach to analyze the functional human gut microbiota”. PLoS One3 (2011): e17447.
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Citation

Citation: Shakira Ghazanfar., et al. “Role of Next Generation Sequencing (NGS) in Searching for Promising Lactic Acid Strains Used as Animal Probiotic". Acta Scientific Nutritional Health 4.4 (2020): 179-183.




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