Hasan Karsen1*, İsmail Koyuncu2, İrfan Binici1, Emine Ayca Güler1, Hakim Çelik3 and Şahbettin Selek4
1Department of Infectious Diseases, Faculty of Medicine, Harran University, Şanlıurfa, Turkey
2Department of Biochemistry, Faculty of Medicine, Harran University, Şanlıurfa, Turkey
3Department of Physiology, Faculty of Medicine, Harran University, Şanlıurfa, Turkey
4Department of Biochemistry, Bezmialem Vakıf University, İstanbul, Turkey
*Corresponding Author: Hasan Karsen, Department of Infectious Diseases, Faculty of Medicine, Harran University, Şanlıurfa, Turkey.
Received: February 24, 2020; Published: February 28, 2020
Background: Our aim is to investigate whether brucellosis causes carcinogenesis through studying the oxidative/anti-oxidative level and DNA damage in different serological titers of brucellosis.
Material and Methods: In the different serological test titers of brucellosis standard agglutination test (SAT): 1/40 = 12 people, 1/80 = 13 people, 1/160 = 13 people, 1/320 = 12 people, 1/640 = 12 people) and 19 healthy people were included in the study. DNA damage was investigated through 8-hydroxy-2'-deoxyguanosine Cayman’s ELISA kit method. The total oxidative status (TOS), total antioxidant capacity (TAC), total thiol (SH) and lipid hydroperoxide (LOOH) levels were analyzed through colorimetric method. Also, oxidative stress index (OSI) was found via calculation.
Results: It was realized that the TOS, LOOH, OSI and DNA damage gradually increased beginning from the control group and brucella SAT 1/40 titer and gained statistical significance (respectively at the 1/160, 1/640, 1/160, 1/80 titers). (p < 0.001). The TAC and SH however, was found to gradually decrease beginning from the control group and brucella SAT 1/40 titer and gain statistical significance (respectively, at the 1/320, 1/640 titers). (p < 0.001). There was a significantly positive correlation between serum brucella SAT titers and DNA damage, TOS, OSI and LOOH (respectively, rho = 0.796, 0.810, 0.389, 0.717). And there was significantly negative correlation between brucella SAT titers and TAC and SH (respectively, rho = -0.540, -0.503).
Conclusion: The serological antibody titer increased in brucellosis, oxidative stress and DNA damage also increased in relation to it. And this DNA damage might lead to brucellosis-related mutation and carcinogenesis. Therefore, these patients must be treated urgently to counteract the DNA damage.
Keywords: Brucellosis; Oxidative Stress; DNA Damage; Carcinogenesis
Citation: Hasan Karsen., et al. “Increased DNA Damage and Oxidative Stress in Patients with Brucellosis". Acta Scientific Nutritional Health 4.4 (2020): 02-06.
Copyright: © 2020 Hasan Karsen., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.