Acta Scientific Nutritional Health (ASNH)(ISSN: 2582-1423)

Research Article Volume 4 Issue 3

Identification and Phylogenetic Studies of a New Probiotic Lactobacillus spp. Egyptian Isolate Based on 16s rRNA Gene

Ahmed R Awd1, Khater A Khater1 and Ahmed I Ismail2,3*

1Dairy Science and Technology Department, Faculty of Agricultural, Al-Azhar University, Egypt
2Biotechnology Department, Faculty of Agriculture, University of Al-Azhar, Cairo, Egypt
3Public Health Department, College of Applied Medical Sciences, Majmaah University, Al-Majmaah, Saudi Arabia

*Corresponding Author: Ahmed I Ismail, Biotechnology Department, Faculty of Agriculture, University of Al-Azhar, Cairo, Egypt and Public Health Department, College of Applied Medical Sciences, Majmaah University, Al-Majmaah, Saudi Arabia.

Received: February 17, 2020; Published: February 26, 2020

×

Abstract

  Lactobacillus represents one of the most important genera of human and animal intestinal tract. They are used in dairy and non-dairy probiotics foods to restore the intestinal microflora, which provides a health benefit. Lactobacillus strains were phenotypically identified and described using biochemical features and kits such as the API 50 CH system. Conventional biochemical and physiological studies have certain limitations when discriminating against large numbers of isolates with similar physiological characteristics. They are generally unreliable, and consuming money, time, and effort. There are many studies have focused on the rapid identification of molecular biology techniques for the rapid identification and detection of lactobacilli. In this study, we examined five pairs of primers on complex communities to facilitate the examination of complex micro-ecosystems. The two primers Lac1-Lac2 and Joh16SI-Joh16II showed positive results with L. casei, L. rhamnosus, and L. gasseri. Their strains were isolated from raw milk and feces of breast-fed infants. The fragments obtained from DNA samples after PCR amplification were sequenced and submitted in GenBank "NCBI" under accessions number "KY123805", "KY123789" and "KY123806". As the long-term goal of this research was to obtain patent protection for Lactobacillus sp. isolated from Egyptian resources and using it in food and dairy industries.

Keywords:API 50 CH; GenBank; Intestinal Microflora; Lactobacillus ; Molecular Identification; Phylogenetic; Probiotics; 16S rRNA

×

References

  1. Anas M., et al. “Antimicrobial activity of Lactobacillus species isolated from Algerian raw goat’s milk against Staphylococcus aureus”. World Journal of Dairy and Food Sciences 3.2 (2008): 39-49.‏
  2. Bernardeau M., et al. “Safety assessment of dairy microorganisms: The Lactobacillus genus”. International Journal of Food Microbiology 126.3 (2008): 278-285.
  3. Felis GE., et al. “Taxonomy of lactobacilli and bifidobacteria”. Current Issues in Intestinal Microbiology 8.2 (2007): 44.‏
  4. Shintaro M., et al. “Genomic characterization of a fructophilic bee symbiont Lactobacillus kunkeei reveals its niche-specific adaptation”. Systematic and Applied Microbiology 39.8 (2016): 516-526.
  5. Klein G., et al. “Taxonomy and physiology of probiotic lactic acid bacteria”. International Journal of Food Microbiology 41.2 (1998): 103-125.
  6. Awaad AM., et al. “Identification of Bifidobacterium Animalis Ssp. Lactis from Egyptian Women Breast Milk and Feces of Breast Fed Infant Based On 16S-23S rRNA Gene”. Advances in Nutrition and Food Science 1.1 (2016): 1-8.
  7. DE Man C., et al. “A medium for the cultivation of lactobacilli”. Journal of Applied Bacteriology 22 (1960): 130-136.
  8. Brock TD., et al. “Cell Biology”. In Biology of Microorganisms. Prentice Hall Int. Editions. Toronto, Canada: (1994): 43.
  9. Herrero M., et al. “Evaluation of technologically important traits in lactic acid bacteria isolated from spontaneous fermentations”. Journal of Applied Bacteriology 81.5 (1996): 565-570.
  10. Bergey's Manual of Determinative Bacteriology. Ninth Edition. 428 East Preston street Baltimore, Maryland 21202, U.S.A.: Williams and Wilkins; (1994).
  11. Collins CH., et al. “Collins and Lyne's microbiological methods”. Butterworth and Co Ltd; (2004).
  12. Walter J., et al. “Detection and Identification of Gastrointestinal Lactobacillus Species by Using Denaturing Gradient Gel Electrophoresis and Species-Specific PCR Primers”. Applied and Environmental Microbiology 66.1 (2000): 297-303.‏
  13. McOrist AL., et al. “A comparison of five methods for extraction of bacterial DNA from human faecal samples”. Journal of Microbiological Methods 50.2 (2002): 131-139.
  14. Tilsala-Timisjärvi A., et al. “Development of oligonucleotide primers from the 16S-23S rRNA intergenic sequences for identifying different dairy and probiotic lactic acid bacteria by PCR”. International journal of Food Microbiology 35.1 (1997): 49-56.‏
  15. Ogunshe AA. “Bioinihibition of diarrhogenic Gram-positive bacteria pathogens indigenous probiotics in industrial infant weaning food”. Asian Pacific Journal of Tropical Medicine 1.2 (2008): 7-11.
  16. Ozgun D., et al. “Identification of Lactobacillus strains isolated from faecal specimens of babies and human milk colostrum by API 50 CHL system”. Journal of Medical Genetics 3.3 (2011): 46-49.
  17. Ventura M., et al. “Specific identification and targeted characterization of Bifidobacterium lactis from different environmental isolates by a combined multiplex- PCR approach”. Applied and Environmental Microbiology 67 (2001): 2760-2765.
  18. Haschke F., et al. “Clinical trials prove the safety and efficacy of the probiotic strain Bifidobacterium Bb12 in follow-up formula and growing-up milk”. MonatsschrKinderheikd 146(1998): 26-30.
  19. Schleifer KH., et al. “Phylogeny of the genus Lactobacillus and related genera”. Systematic and Applied Microbiology 18.4 (1995): 461-467.‏
  20. Pot B and Tsakalidou E. “Taxonomy and metabolism of Lactobacillus”. In: Ljungh, A., Wadstro¨m, T. (Eds.), Lactobacillus Molecular Biology: From Genomics to Probiotics. Caister Academic Press, Norfolk, (2009): 3-58‏.
  21. Dellaglio F., et al. “The status of the species Lactobacillus casei”(Orla-Jensen 1916) Hansen and Lessel (1971) and “Lactobacillus paracasei Collins” et al. (1989): request for an opinion”. International Journal of Systematic Bacteriology 52, 285e287.
  22. Desai AR., et al. “Discrimination of dairy industry isolates of the Lactobacillus casei group”. Journal of Dairy Science 89 (2006):3345-3351.
  23. De Vos P., et al. “Judicial commission of the international committee on systematics of prokaryotes, 10th international congress of bacteriology and applied microbiology, minutes of meetings 28, 29 and 31 July and 1 August 2002, Paris, France”. International Journal of Systematic and Evolutionary Microbiology 55 (2005): 525e532. 
  24. Pot B., et al. “Taxonomy of Lactic Acid Bacteria”. In: De Vuyst L., Vandamme E.J. (eds) Bacteriocins of Lactic Acid Bacteria. Springer, Boston, MA (1994).
×

Citation

Citation: Ahmed I Ismail., et al. “Identification and Phylogenetic Studies of a New Probiotic Lactobacillus spp. Egyptian Isolate Based on 16s rRNA Gene". Acta Scientific Nutritional Health 4.3 (2020): 104-110.




Metrics

Acceptance rate30%
Acceptance to publication20-30 days
Impact Factor0.819

Indexed In





News and Events


  • Certification for Review
    Acta Scientific certifies the Editors/reviewers for their review done towards the assigned articles of the respective journals.
  • Submission Timeline for Upcoming Issue
    The last date for submission of articles for regular Issues is May 30, 2021.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Article of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of “Best Article of the Issue”.
  • Welcoming Article Submission
    Acta Scientific delightfully welcomes active researchers for submission of articles towards the upcoming issue of respective journals.
  • Contact US