Acta Scientific Nutritional Health (ASNH)(ISSN: 2582-1423)

Research Article Volume 4 Issue 3

Bioactive Psiadia punctulata Trachylobane Diterpenes Against Mastitis Pathogens

Wanjau OM1*, Mahungu SM2 and Matasyoh JC1

1Department of Chemistry, Faculty of Science, Egerton University, Kenya
2Deparment of Dairy and Food Science, Faculty of Agriculture, Egerton University, Nakuru, Kenya

*Corresponding Author: Wanjau OM, Department of Chemistry, Faculty of Science, Egerton University, Kenya.

Received: February 21, 2020; Published: February 26, 2020

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Abstract

  Bacterial pathogens are a major challenge and a constant drawback in the management of both human and animal health. The dairy farmer may suffer huge losses due to infection of the udder of dairy cows with mastitis. Manifestation of mastitis in dairy cows occurs, resulting from poor personnel hygiene, unsuitable environment, unhygienic milking procedure or use of contaminated milking equipment and supplies. At times managerial measures have failed to give significant success, and use of post milking teat dipping solutions has been applied with success.
  The presence of a leaf surface exudate on Psiadia punctulata is supposedly for a purpose, and this, coupled with the traditional uses for the plant warrant bioassay directed phytochemical studies on the plant.
   Column chromatography of concentrated cold acetone leaf wash of Psiadia punctulata using silica gel; serially eluting the column with ethyl acetate-hexane mixtures, yielded two trachylobane diterpenoid compounds, PP5 and PP6 as clear crystalline compounds. In vitro bioassay tests applying the disc diffusion method and the agar diffusion methods using levofloxacin and chloramphenicol as positive controls found the trachylobane diterpenoid compounds to be active against Staph aureus and Salmonella typhi. Structural characteristics of the diterpenoid compounds were evaluated through phytochemical tests and correlation of proton and carbon-13 NMR spectra. The presence of antimicrobial agents in acetone leaf wash of Psiadia punctulata, active against Staph aureus and Salmonella typhi makes the plant invaluable as a source of a home remedy in eradication of mastitis manifestation in dairy cows.

Keywords: Psiadia punctulate; Antimicrobial; Trachylobane Diterpenes; Mastitis, Staph aureus and Salmonella typhi

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References

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Citation

Citation: Wanjau OM., et al. “Bioactive Psiadia punctulata Trachylobane Diterpenes Against Mastitis Pathogens" Acta Scientific Nutritional Health 4.3 (2020): 99-103.




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