Abstract

  This work evaluated the phytochemical composition of freeze-dried acerola, camu-camu, apple and pineapple extracts, and correlated it with their cell-based in vitro antioxidant, anti-inflammatory and wound healing activities. All freeze-dried fruit extracts at 50 µm/mL significantly inhibited (p<0.001) reactive oxygen species (ROS) up to 70%, but only acerola and camu-camu extracts at 150 µm/mL decreased (p<0.001) the nitric oxide (NO) production (up to 33%) in LPS-stimulated RAW 264.7 macrophages. When selected mRNA biomarkers of acute inflammation were evaluated, all freeze-dried fruit extracts inhibited the expression of IL-6 and IL-1β genes, but only acerola reduced the COX-2 expression (p<0.01). In addition, freeze-dried acerola extract (50 µm/mL) significantly enhanced human dermal fibroblasts (HDFa) migration by 1.5-fold compared to the control after 36 h. Based on our results, we hypothesize that the high concentration of ascorbic acid (9454.3 mg/100 g) and anthocyanin contents (14.0 mg /100 g) of freeze-dried acerola may play an important role on the in vitro biological results.

Keywords: Ascorbic Acid; Inflammation; Acerola; Wound Healing; Edible Fruits

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Citation

Citation: Debora Esposito.,et al. “Phytochemical Content, Antioxidant, Anti-inflammatory Activities and Wound Healing Properties of Freeze-Dried Fruits".Acta Scientific Nutritional Health 4.1 (2020): 63-71.

Copyright

Copyright: © 2020 Debora Esposito.,et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.