Acta Scientific Nutritional Health (ASNH)(ISSN: 2582-1423)

Research Article Volume 4 Issue 1

Regular Intake of Tomato Juice by Egyptian Male Preadolescents was Associated with Significant Increase in the Plasma Lycopene Concentration and Modest Changes in the Blood Glutathione and Plasma Immunoglobulin E Levels

Amr Samir ElBihnesawya, Mostafa Gouda, Laila Hussein

Department of Nutrition and Food Science, National Research Centre, Giza, Egypt

*Corresponding Author: Laila Hussein, Department of Nutrition and Food Science, National Research Centre, Giza, Egypt.

Received: November 20, 2019; Published: December 05, 2019



  The objectives of the present study is to test the impact of nutritional intervention with natural tomato juice on health among preadolescent to adolescent males. The study was coupled with quantitative measurements of selected biomarkers to validate the observed links between the regular consumption of tomato juice and impact on selected biomarkers of health status. Design of the dietary intervention trial The study consisted of 25 boys (10.5 years old), who received tomato juice for a duration of 18 days with mean daily intake of 240 g tomato juice containing 2% corn oil, which supplied 22.3 mg trans lycopene and 1.32 mg β-carotene. Blood samples were collected from the anticubital vein at 7 different time intervals according to a predetermined schedule for the analysis. Carotenoid pigments, immunoglulin E and C- reactive protein were analyzed in the plasma amples, while glutathione (GSH) was determined in the whole blood. The results showed that the plasma lycopene increased gradually with a peak at day 8, which was 2.55 fold in excess of the prefeeding period (day zero). The increases in the plasma concentrations of α and β -carotene reached a peak, which was 1.43 fold. The plasma immunoglobuline E (Ig E) a biomarker of allergy or the effect of infection on threimmune system decreased but the level didn’t reach significant level (P>0.05). The plasma C-reactive protein levels in all children except one child were below the detection limit (< 6 mg/L), Therefore, the analysis was not repeated after the Tomato feeding. With respect to blood GSH, the increases in its concentration peaked on day, but the increase was not significant (P>0.05). Thus, tomato juice could be considered as a potential functional product with a high antioxidant and anti-inflammatory properties but are additional studies for longer period of times are warranted.

Keywords: Male Adolescents; Infection with Intestinal Parasites; Tomato Juice; Tomato Lycopene; Plasma Lycopene; Blood Glutathione; Plasma Immunoglobulin E; C –Reactive Proteins



  1. Salehi B., et al. "Beneficial Effects and Potential Risks of Tomato Consumption for Human Health: An Overview”. Nutrition 62 (2019): 201-208.
  2. Perveen R., et al. "Tomato (Solanum Lycopersicum) Carotenoids and Lycopenes Chemistry; Metabolism, Absorption, Nutrition, and Allied Health Claims--a Comprehensive Review”. Critical Reviews in Food Science and Nutrition 7 (2015): 919-929.
  3. Ganji V., et al. "Population Determinants of Serum Lycopene Concentrations in the United States: Data from the Third National Health and Nutrition Examination Survey, 1988-1994”. Journal of Nutrition3 (2005): 567-572.
  4. Riso P., et al. "Lycopene and Vitamin C Concentrations Increase in Plasma and Lymphocytes after Tomato Intake. Effects on Cellular Antioxidant Protection”. European Journal of Clinical Nutrition10 (2004): 1350-1358.
  5. Gann PH., et al. "Lower Prostate Cancer Risk in Men with Elevated Plasma Lycopene Levels: Results of a Prospective Analysis”. Cancer Research6 (1999): 1225-1230.
  6. Turati F., et al. "Fruit and Vegetables and Cancer Risk: A Review of Southern European Studies”. British Journal of Nutrition 113 (2015): S102-110.
  7. Reimers K., et al. "Relationship of the Tomato Intake Amount Recommended in Myplate to Cardiovascular Disease Risk Reduction”. Faseb Journal1 (2014).
  8. Costa-Rodrigues J., et al. "Modulation of Human Osteoclastogenesis and Osteoblastogenesis by Lycopene”. Journal of Nutrition and Biochemistry 57 (2018): 26-34.
  9. Petyaev IM. "Lycopene Deficiency in Ageing and Cardiovascular Disease”. Oxidative Medicine and Cellular Longevity (2016): 3218605.
  10. Silaste ML., et al. "Tomato Juice Decreases Ldl Cholesterol Levels and Increases Ldl Resistance to Oxidation”. British Journal of Nutrition6 (2007): 1251-1258.
  11. Bub A., et al. "Paraoxonase 1 Q192r (Pon1-192) Polymorphism Is Associated with Reduced Lipid Peroxidation in Healthy Young Men on a Low-Carotenoid Diet Supplemented with Tomato Juice”. British Journal of Nutrition3 (2005): 291-297.
  12. Hussein L., et al. "Normal Distribution of Urinary Polyphenol Excretion among Egyptian Males 7-14 Years Old and Changes Following Nutritional Intervention with Tomato Juice (Lycopersicon Esculentum)”. International Journal of Food Sciences and Nutrition4 (2009): 302-311.
  13. Yamamoto T., et al. "Anti-Allergic Activity of Naringenin Chalcone from a Tomato Skin Extract”. Bioscience, Biotechnology, and Biochemistry8 (2004): 1706-1711.
  14. Riso P., et al. "Effect of a Tomato-Based Drink on Markers of Inflammation, Immunomodulation, and Oxidative Stress”. Journal of Agricultural and Food Chemistry 7 (2006): 2563-2566.
  15. Ghavipour M., et al. "Tomato Juice Consumption Reduces Systemic Inflammation in Overweight and Obese Females”. British Journal of Nutrition11 (2013): 2031-2035.
  16. Valderas-Martinez P., et al. "Tomato Sauce Enriched with Olive Oil Exerts Greater Effects on Cardiovascular Disease Risk Factors Than Raw Tomato and Tomato Sauce: A Randomized Trial”. Nutrients3 (2016): 170.
  17. U.S. Department of Agriculture, Agricultural Research Service, National Nutrient Database for Standard Refer-Ence, Release 18, Nutrient Data Laboratory Homepage (2005).
  18. Craft NE., et al. “Effects of Storage and Handling Conditions on Concentrations of Individual Carotenoids, Retinol, and Tocopherol in Plasma”. Clinical Chemistry1 (1988): 44-48.
  19. Beutler E., et al. “Improved Method for the Determination of Blood Glutathione”. Journal of Laboratory and Clinical Medicine 61 (1963): 882-888.
  20. Khachik F., et al. "Chemistry, Distribution, and Metabolism of Tomato Carotenoids and Their Impact on Human Health”. Experimental Biology and Medicine10 (2002): 845-851.
  21. Sadler G., et al. “Rapid Extraction Oflycopene Andb-Carotene from Reconstituted Tomato Pasteand Pink Grapefruit Homogenates”. Food Science 55 (1990): 1460-1461.
  22. Foppen FH. "Tables for the Identification of Carotenoid Pigments”. Chromatography Review3 (1971): 133-298.
  23. Hussein Laila., et al. "Dyslipidemia and Other Risk Factors among Egyptian Patients with Type-2 Diabetes Mellitus and the Impact of Dietary Intervention with Thermally Treated Tomato Juice”. International Journal of Clinical Nutrition and Dietetics1 (2018).
  24. Boileau AC., et al. “Cis-Lycopene Is More Bioavailable Than Trans-Lycopene in Vitro and in Vivo in Lymph-Cannulated Ferrets”. Journal of Nutrition6 (1999): 1176-1181.
  25. Chen J., et al. “Effect of Lycopene Supplementation on Oxidative Stress: An Exploratory Systematic Review and Meta-Analysis of Randomized Controlled Trials”. Journal of Medicinal Food 5 (2013): 361-374.
  26. Pizzorno J. "Glutathione!" Journal of Integrative Medicine (Encinitas) 13.1 (2014): 8-12.
  27. Mari M., et al. "Mitochondrial Glutathione, a Key Survival Antioxidant”. Antioxidants and Redox Signaling11 (2009): 2685-2700.
  28. Demirkol O., et al. “Biologically Important Thiols in Various Vegetables and Fruits”. Journal of Agricultural and Food Chemistry26 (2004): 8151-8154.
  29. Lavoie S., et al. "Erythrocyte Glutathione Levels as Long-Term Predictor of Transition to Psychosis”. Translational Psychiatry 7 (2017).
  30. Giustarini D., et al. "Detection of Glutathione in Whole Blood after Stabilization with N-Ethylmaleimide”. Analytical Biochemistry1 (2011): 81-83.
  31. Jacob K., et al. "Influence of Lycopene and Vitamin C from Tomato Juice on Biomarkers of Oxidative Stress and Inflammation”. British Journal of Nutrition1 (2008): 137-146.
  32. Lindberg RE and C Arroyave. "Levels of Ige in Serum from Normal-Children and Allergic Children as Measured by an Enzyme-Immunoassay”. Journal of Allergy and Clinical Immunology 78 4 (1986): 614-618.
  33. Ahmed I and S Nasreen. "Frequency of Raised Serum Ige Level in Childhood Atopic Dermatitis”. Journal of Pakistan Medical Association9 (2007): 431-434.
  34. Shankar V., et al. "Differences in Gut Metabolites and Microbial Composition and Functions between Egyptian and U.S. Children Are Consistent with Their Diets”. Msystems 1 (2017).
  35. Morales-Espinoza EM., et al. "Intestinal Par-Asites in Children, in Highly Deprived Areas in the Border Region of Chiapas, Mexico”. Salud Pública de México 45 (2003): 379-88.
  36. Pinot de Moira A., et al. "Effects of Treatment on Ige Responses against Parasite Allergen-Like Proteins and Immunity to Reinfection in Childhood Schistosome and Hookworm Coinfections”. Infection and Immunity 1 (2013): 23-32.
  37. Vehapoglu A., et al. "Reduced Antioxidant Capacity and Increased Subclinical Inflammation Markers in Prepubescent Obese Children and Their Relationship with Nutritional Markers and Metabolic Parameters”. Redox Report6 (2016): 271-280.


Citation: Laila Hussein.,et al. “Regular Intake of Tomato Juice by Egyptian Male Preadolescents was Associated with Significant Increase in the Plasma Lycopene Concentration and Modest Changes in the Blood Glutathione and Plasma Immunoglobulin E Levels".Acta Scientific Nutritional Health 4.1 (2020): 07-13.


Acceptance rate30%
Acceptance to publication20-30 days
Impact Factor1.316

Indexed In

News and Events

  • Certification for Review
    Acta Scientific certifies the Editors/reviewers for their review done towards the assigned articles of the respective journals.
  • Submission Timeline for Upcoming Issue
    The last date for submission of articles for regular Issues is July 10, 2024.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Article of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of "Best Article of the Issue"
  • Welcoming Article Submission
    Acta Scientific delightfully welcomes active researchers for submission of articles towards the upcoming issue of respective journals.

Contact US