Hawa Alsadi* and Salem Mohamed Edrah*

Chemistry Department, Science College, Education College, Elmergib University, Alkhums Libya

*Corresponding Author: Salem Mohamed Edrah, Hawa Alsadi, Chemistry Department, Science College, Education College, Elmergib University, Alkhums Libya.

Received: March 10, 2022; Published: April 18, 2022

Abstract

Objectives: Extraction of oil from Linum u. seeds, investigating, characterizing of this oil by using chemical and extensive spectroscopic methodologies.

Methods: The oil constituents were isolated by using the Soxhlet Apparatus Extractor in n-hexane as a solvent, and diagnosis, characterization by the Gas Chromatography Technique (GC), High-Performance Liquid Chromatography (HPLC), FTIR, H¹, C¹³ NMR, Oil Thermal Analysis (DSC) and Determination of Solid Fat Content (SFC %).

Results: The percentage yield of oil content of Linum u. was 39%. The Physiochemical Properties, the acid value was 4.4 mg/g, Free Fatty Acid content (FFA %) free oleic acid was 2.2%, the average molecular weight value was 848 and the density of oil ranged from 0.89 g/cm³. According to triacylglycerols profiles (TAG)s in the oil were polyunsaturated of OLL with 17.45%, LnLnLn with 20.65 - 21.44%, OLnLn with 8.18 - 8.84%, PLLn with 6.72%, PLnLn with 7.97%. Whereas the FTIR Spectrum results of the presence of the functional groups of triacylglycerols were peaks at 3010- 3020, 2927-924, 1743-1743, 1462-1463, 1376-1377, 1238-1250, 1100-1110, and 722-721 cm^-1. Thermal Transition Properties for quality of the Linum u. oil after melting in the Differential Scanning Calorimetric were showed a simple thermo-gram. While the obtained results from the p NMR analysis of the Solid Fat Contents were decreased when the temperature increased until it reaches room temperature 25⁰C, which means that the oil contains higher unsaturated fatty acid 89.5% more than the saturated fatty acid 10.5%. Whereas by H¹ and C¹³ NMR chemical shifts were observed that there were no significant differences between the Linum u. oil from Libya and Linum u. oil from Canada, and where both oils contain triacylglycerol TAG in which the NMR chemical shifts were consistent. Accordingly, the H¹ NMR spectrum shows the chemical shift at 0.868-0.986 ppm and 0.5 ppm referred to the methylene group, respectively, and the terminal methyl group which is between 1.25- 1.343 ppm and 1.0 ppm, and for the methylene 2.005 ppm and 2.0 ppm, the acyl groups were at 5.253-5.39 ppm and 5.2 ppm. As well as the methylene groups of glyceryl were at 4.281-4.308 ppm and 4.5 ppm. In addition, the C¹³ NMR spectrum shows the main signals assignment at 172.76-173.17ppm refers to the carbon atom of the carbonyl group, and the signals at 127.12-131.92 ppm refer to the unsaturated carbon atoms 62.09-68.92 ppm due to the glyceryl carbon atoms, and were the methylene carbon atoms were further distinguished into those adjacent to one double bond, saturated at 29.0-29.7 ppm. As well, the results obtained by Gas Chromatography (G C) were 89.4% for unsaturated fatty acid and 10.1% for saturated fatty acid. And the unsaturated fatty acids present in the oil are dominated by 55% linoleic acid (C18.3), 15.9% linoleic acid and 17.8% oleic acid. Meanwhile, saturation fatty acids, present in the Linum u. oil consisted of 5.3% Palmitic acid (C16:0) and 4.4% Stearic acid (C18:0). The HPLC results showed that LnLnLn (21%) was dominated by triacylglycerols.

Conclusion: The findings of the study revealed that Linum u. oil has the potential uses as a source of pharmaceutical for treating diseases. Therefore flaxseed plants can be further assayed for pharmacological preparations.

Keywords: Linum usitatissimum (Linum u.); HPLC; G C; FTIR; NMR; Oil Thermal Analysis; Solid Fat Content

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Citation

Citation: Hawa Alsadi and Salem Mohamed Edrah. “Diagnosis and Characterization by Using NMR, FT-IR, HPLC and G C Spectroscopies for Flaxseeds (Brown Variety) Oil Content from Libya". Acta Scientific Medical Sciences 6.5 (2022): 98-108.

Copyright

Copyright: © 2022 Hawa Alsadi and Salem Mohamed Edrah. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.