Keerthy M¹*, M Arya Lakshmi¹ and B Ganga Rao²

¹Pharmacognosy and Phytochemistry Research Division, University College of Pharmaceutical Sciences, Andhra University, Andhra Pradesh, India
²Principal, KJR college of Pharmacy, Andhra Pradesh, India

*Corresponding Author: Keerthy M, Pharmacognosy and Phytochemistry Research Division, University College of Pharmaceutical Sciences, Andhra University, Andhra Pradesh, India.

Received: August 02, 2021; Published: September 22, 2021

Abstract

  Dengue is the most widely spread arthropod-transmitted viral diseases of human beings, approximately 390 million people are getting symptomatic infections annually and more than 2 billion people are living in risk conditions. Specified antiviral drug has not approved yet against dengue and in some countries restricted introduction of dengue vaccine is the only source. the only there is only limited introduction of a dengue vaccine in some countries. Bacopa monnieri (L.) Wettst plant frequently called as Brahmi and water hyssop, belongs to the family of Plantaginaceae. Bacopa monnieri (L.) Wettst is used as a medicine to cure various conditions including infections. In this study, determination of maximum non-toxic dose (MNTD) of the Bacopa monnieri (L.) Wettst was done against Vero E6 cells under in vitro conditions with the methanolic extract. The antiviral assay of Bacopa monnieri (L.) Wettst based on cytopathic effects (CPE) represented by degree of inhibition against DENV serotype 2 strain. Results showed that Bacopa monnieri (L.) Wettst had significant anti-dengue activity in Vero E6 cell lines by lowering the levels of cellular infection as well as virus output. It is done with the 50% effective concentrations for DENV 2 for Vero E6 cell lines. These results of this study by using MTT assay method, in which the Percentage of inhibition in the presence of Bacopa monnieri methanolic extract for Anti DENV-2 activity was increased dose dependent manner from 1.56 to 100 µg in DENV-2 infected cell. The results indicated that Bacopa monnieri (L.) Wettst has a potentiality as an anti-viral agent against dengue infection for further development.

Keywords: Dengue Virus DENV 2; Bacopa monnieri (L.) Wettst; Plantaginaceae; Antiviral

References

1. Waggoner JJ., et al. “Viremia and Clinical Presentation in Nicaraguan Patients Infected with Zika Virus, Chikungunya Virus, and Dengue Virus”. Clinical Infectious Diseases 12 (2016): 1584-1590.
2. Bhatt, S., et al. “The global distribution and burden of dengue”. Nature7446 (2013): 504-507.
3. Brady OJ., et al. “Refining the global spatial limits of dengue virus transmission by evidence-based consensus”. PLOS Neglected Tropical Diseases8 (2012): e1760.
4. Wilder-Smith A., et al. “DengueTools: Innovative tools and strategies for the surveillance and control of dengue”. Global Health Action 5 (2012): 17273.
5. Guzman MG., et al. “Secondary infection as a risk factor for dengue hemorrhagic fever/dengue shock syndrome: An historical perspective and role of antibody-dependent enhancement of infection”. Archives of Virology 158 (2013): 1445-1459.
6. Badurdeen S., et al. “Sharing experiences: Towards an evidence-based model of dengue surveillance and outbreak response in Latin America and Asia”. BMC Public Health 13 (2013): 607.
7. Cos P., et al. “Anti-infective potential of natural products: How to develop a stronger in vitro ‘proof of concept”. Journal of Ethnopharmacology 106 (2006): 290-302.
8. World Health Organization. Comprehensive Guidelines for Prevention and Control of Dengue and Dengue Haemorrhagic Fever: Revised and Expanded Edition; SEARO: New Delhi, India, (2011).
9. Ghosh A and Dar L. “Dengue vaccines: Challenges, development, current status and prospects”. Indian Journal of Medical Microbiology 33 (2015): 3-15.
10. De Maio FA., et al. “The dengue virus NS5 protein intrudes in the cellular spliceosome and modulates splicing”. PLoS Pathogen 12 (2016): e1005841.
11. Singh AG., et al. “An ethnobotanical survey of medicinal plants used in Terai forest of Western Nepal”. Journal of Ethnobiology and Ethnomedicine 8 (2012): 19.
12. Bhowmik D., et al. “Traditional Indian memory enhancer herbs and their medicinal importance”. Annals of Biological Research1 (2010): 41-46.
13. Shah GM., et al. “Traditional uses of medicinal plants against malarial disease by the tribal communities of Lesser Himalayas- Pakistan”. Journal Ethnopharmacology1 (2014): 450-462.
14. Deepak M., et al. “Quantitative determination of the major saponin mixture bacoside A in B. monnieri by HPLC”. Phytochemistry Analyst1 (2005): 24-29.

Citation

Citation: Keerthy M., et al. “In vitro Antiviral Activity of Bacopa monnieri (l.) Wettst Roots Methanolic Extract Against Dengue Virus Type 2”.Acta Scientific Medical Sciences 5.10 (2021): 114-119.

Copyright

Copyright: © 2021 Keerthy M., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.