Keerthy M1*, M Arya Lakshmi1 and B Ganga Rao2
1Pharmacognosy and Phytochemistry Research Division, University College of Pharmaceutical Sciences, Andhra University, Andhra Pradesh, India
2Principal, KJR college of Pharmacy, Andhra Pradesh, India
*Corresponding Author: Keerthy M, Pharmacognosy and Phytochemistry Research Division, University College of Pharmaceutical Sciences, Andhra University, Andhra Pradesh, India.
Received: August 02, 2021; Published: September 22, 2021
Dengue is the most widely spread arthropod-transmitted viral diseases of human beings, approximately 390 million people are getting symptomatic infections annually and more than 2 billion people are living in risk conditions. Specified antiviral drug has not approved yet against dengue and in some countries restricted introduction of dengue vaccine is the only source. the only there is only limited introduction of a dengue vaccine in some countries. Bacopa monnieri (L.) Wettst plant frequently called as Brahmi and water hyssop, belongs to the family of Plantaginaceae. Bacopa monnieri (L.) Wettst is used as a medicine to cure various conditions including infections. In this study, determination of maximum non-toxic dose (MNTD) of the Bacopa monnieri (L.) Wettst was done against Vero E6 cells under in vitro conditions with the methanolic extract. The antiviral assay of Bacopa monnieri (L.) Wettst based on cytopathic effects (CPE) represented by degree of inhibition against DENV serotype 2 strain. Results showed that Bacopa monnieri (L.) Wettst had significant anti-dengue activity in Vero E6 cell lines by lowering the levels of cellular infection as well as virus output. It is done with the 50% effective concentrations for DENV 2 for Vero E6 cell lines. These results of this study by using MTT assay method, in which the Percentage of inhibition in the presence of Bacopa monnieri methanolic extract for Anti DENV-2 activity was increased dose dependent manner from 1.56 to 100 µg in DENV-2 infected cell. The results indicated that Bacopa monnieri (L.) Wettst has a potentiality as an anti-viral agent against dengue infection for further development.
Keywords: Dengue Virus DENV 2; Bacopa monnieri (L.) Wettst; Plantaginaceae; Antiviral
Citation: Keerthy M., et al. “In vitro Antiviral Activity of Bacopa monnieri (l.) Wettst Roots Methanolic Extract Against Dengue Virus Type 2”.Acta Scientific Medical Sciences 5.10 (2021): 114-119.
Copyright: © 2021 Keerthy M., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.