PICSI: A Boon for Quality Sperm Selection?
Atita Shinde1* and Shivnath Yadav2
1Research Scholar, Sunrise University, Alwar, Rajasthan
2Research Supervisor, Sunrise University, Alwar, Rajasthan
*Corresponding Author: Shinde, Research Scholar, Sunrise University, Alwar, Rajasthan.
Received:
November 21, 2023; Published: December 04, 2023
Abstract
Fertilization is possible in cases in which the sperm motility and ability to penetrate the zona pellucid are impaired. PICSI has now a day’s became method of choice to achieve fertilization. Hyaluronan (HA), a significant ingredient of the cumulus matrix plays a crucial role in the selection of functionally competent sperm for in vitro fertilization. Here HA-bound sperm carry increased levels of developmental maturity and sperm integrity.
The PICSI dish can be used for standard ICSI procedure and is a sterile plastic dish containing 3 microdots of Hyaluronic acid, where only mature DNA intact spermatozoa will bind for easy picking.
Keywords: Fertilization; Hyaluronan (HA); DNA
References
- Anand PS., et al. “Effect of dietary supplementation of biofloc on growth performance and digestive enzyme activities in Penaeus monodon”. Aquaculture 418 (2014): 108-115.
- “In Official Methods of Analysis, 18th edition”. (Association of Analytical Chemists). Washington (2011).
- Avnimelech Y. “Carbon/nitrogen ratio as a control element in aquaculture systems”. Aquaculture3-4 (1999): 227-235.
- Avnimelech Y. “Biofloc Technology-A Practical Guide Book”. Baton Rouge, LA: The World Aquaculture Society (2009):
- Avnimelech Y. “Biofloc technology - a practical guide book. Edition”. 2nd Baton Rouge, United States: The World Aquaculture Society (2012).
- Costa‐Pierce BA., et al. “Responsible use of resources for sustainable aquaculture. In Proceedings of the Global Conference on Aquaculture 2010: Farming the Waters for People and Food. Subasinghe R.P., Arthur J.R., Bartley D.M., De Silva S.S., Halwart M., Hishamunda N., Mohan C.V., Sorgeloos P., (eds). Rome, Italy: Food and Agriculture Organization of the United Nation (2012): 113-436.
- Dash P., et al. “The addition of probiotic bacteria to microbial floc: Water quality, growth, non-specific immune response and disease resistance of Cyprinus carpio in mid-Himalayan altitude”. Aquaculture 495 (2018): 961-969.
- De Schryver P., et al. “The basics of bio-flocs technology: the added value for aquaculture”. Aquaculture277.3-4 (2008): 125-137.
- Eaton AD., et al. “APHA AWWA WEF Standard Methods for the examination of Water and Waste Water. 19th edition, Washington, DC, USA.
- Ebeling JM., et al. “Engineering analysis of the stoichiometry of photoautotrophic, autotrophic, and heterotrophic removal of ammonia-nitrogen in aquaculture systems”. Aquaculture1-4 (2006): 346-358.
- Kuhn DD., et al. “Evaluation of two types of bioflocs derived from biological treatment of fish effluent as feed ingredients for Pacific white shrimp, Litopenaeus vannamei”.Aquaculture1-4 (2010): 28-33.
- Poonam Dahiya T., et al. “Physico-chemical properties of water in biofloc supplemented with probiotics: A review”. Journal of Expeimental Zoology. India 24 (2021): 843-851.
- Verdegem MC. “Nutrient discharge from aquaculture operations in function of system design and production environment”. Reviews in Aquaculture5.3 (2013): 158-171.
- Wang G., et al. “Effect of C/N ratio on water quality in zero-water exchange tanks and the biofloc supplementation in feed on the growth performance of crucian carp, Carassius auratus”. Aquaculture443 (2015): 98-104.
- Wei Y., et al. “The effect of different carbon sources on the nutritional composition, microbial community and structure of bioflocs. Aquaculture 465 (2016): 88-93.
- Xu WJ and Pan LQ. “Effects of bioflocs on growth performance, digestive enzyme activity and body composition of juvenile Litopenaeus vannamei in zero-water exchange tanks manipulating C/N ratio in feed”. Aquaculture 356 (2012): 147-152.
- Zhao P., et al. “The application of bioflocs technology in high-intensive, zero exchange farming systems of Marsupenaeus japonicus”. Aquaculture 354 (2012): 97-106.
- Zhao S., et al. “Application of machine learning in intelligent fish aquaculture: A review”. Aquaculture 540 (2014):
Citation
Copyright