Acta Scientific Women's Health (ASWH)(ISSN: 2582-3205)

Research Article Volume 4 Issue 7

Effects of Quercetin Nanoparticles, An Antioxidant Bioflavonoid on the Retina of STZ-induced Diabetic Rats

Anhar M Gomaa1, Soheir N Abd El-Rahman2* and Suhailah S Al-Jameel3

1Research Institute of Ophthalmology, Giza, Egypt
2Crops Technology Research Department, Food Technology Research Institute, Agricultural Research Center, Giza, Egypt
3Department of Chemistry, College of Science, Imam Abdulrahman Bin Faisal University, Saudi Arabia

*Corresponding Author: Soheir N Abd El-Rahman, Crops Technology Research Department, Food Technology Research Institute, Agricultural Research Center, Egypt, Mail:soheirkenawy@yahoo.com, https://orcid.org/0000-0001-8056-254.

Received: April 19, 2022; Published: June 17, 2022

Abstract

The retina is susceptible to oxidative stress due to an increase in free radicals or deficiency in natural antioxidants, induced in diabetic retinopathy. So, this study is aimed to examine the beneficial effect of Quercetin Nanoparticles (QUNPs) to protect the retina against diabetic rats and its oxidative stress. Diabetic rats were treated with QUNPs (5 and 10 mg/kg BW) for 8 weeks, then the fasting blood sugar (FBS), lipid markers (TC, TG, LDL-c, and HDL-c), were determined at the end of the experiment. Superoxide dismutase; (SOD), Catalase; (CAT) activities; in addition to glutathione; (GSH) and malondialdehyde (MDA) were determined in the blood and retina. Histological changes in the retina were evaluated by a light microscopic (LM) study. Our results revealed that QUNPs treatment for diabetic rats caused a significantly decreased in FBS, TC, TG, LDL-c levels. Also, a highly significant increase in HDL-c level was noticed. QUNPs (10 mg/kg BW) +STZ (60 mg/kg BW) group results are the same more or less normal values in MDA, CAT, SOD, and GSH compared to a positive control (PC) and positive modulation of anti-oxidant enzyme activity was noticed. Moreover, QUNPs treatment which was an effective antioxidant did not have negative effects on the health of rats. Light microscopic study showed thickness increased in basement membrane in the diabetic retina, while relatively thin LM was observed in QUNPs 5 and 10 mg/kg BW- retina treated group. It can be concluded that QUNPs can be effective for the protection of diabetes-induced neurovascular complications such as diabetic retinopathy. Therefore, our study suggests that QUNPs can play an important role as natural nano-antioxidant of oxidative stress in diabetic retinopathy.

Keywords:Diabetic Retinopathy; Oxidative Stress; Quercetin Nanoparticles; Streptozotocin (STZ)

References

  1. Yeğin S and Mert N. “Investigation on the HbA1c, MDA, GSH-Px and SOD Levels in Experimentally Diabetic Rats”. Yüzüncü yıl Üniversitesi Veteriner Fakültesi Dergisi 2 (2013): 51-54.
  2. Ruiz C., et al. “Lipid peroxidation and antioxidant enzyme activities in patients with type-I diabetes mellitus”. Scandinavian Journal of Clinical and Laboratory Investigation 59 (1994): 99-105.
  3. Kern T., et al. “Response of capillary cell death to aminoguanidine predicts the development of retinopathy: comparison of diabetes and galactosemia”. Investigative Ophthalmology and Visual Science 41 (2000): 3972-3978.
  4. Yu ZY., et al. “Streptozotocin induceddiabetic retinopathy in C57 mice and the expression of some pro -angiogenic molecules”. Guoji Yanke Zazhi (Int Eye Sci)1 (2016): 1-6.
  5. Kelly G. “Quercetin Alternative Medicine Review Ehrlich SD. Quercetin”. University of Maryland Medical 16 (2017): 172-194.
  6. Plumb W., et al. “Antioxidant properties of flavonol glycosides from green beans”. Redox Report 4 (1999): 123-127.
  7. Hanasaki Y., et al. “The correlation between active oxygen scavenging and antioxidative effects of flavonoids”. Free Radical Biology and Medicine 6 (1994): 845-853.
  8. Bentz AR. “Review of quercetin chemistry, antioxidant properties, and bioavailability Flavonoids. Wako Laboratory”. Journal of Young Investigators (2009).
  9. Morand C., et al. “Plasma metabolites of quercetin and their antioxidant properties”. American Journal of Physiology 75 (1998): R212-219.
  10. Ebrahimpour S., et al. “Quercetin‑conjugated superparamagnetic iron oxide nanoparticles (QCSPIONs) increases Nrf2 expression via miR‑27a mediation to prevent memory dysfunction in diabetic rats”. Scientific Reports 10 (2020): 15957.
  11. Refat MS., et al. “Quercetin/Zinc complex and stem cells: A new drug therapy to ameliorate glycometabolic control and pulmonary dysfunction in diabetes mellitus: Structural characterization and genetic studies”. PLoS ONE3 (2021): e0246265.
  12. Soheir NA and Suhailah SA. “Quercetin Nanoparticles: Preparation and Characterization”. Indian Journal of Drugs 2.3 (2014): 96-103.
  13. Allain C., et al. “Enzymatic determination of total serum cholesterol”. Journal of Clinical Chemistry 20 (1974): 470-275.
  14. Fossati P and Prencipe L. “The determination of triglycerides using enzymatic methods”. Journal of Clinical Chemistry 28 (1982): 2077.
  15. Ohkawa H., et al. “Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction”. Analytical Biochemistry 95 (1979): 351-358.
  16. Ohkuma N., et al. “Superoxide dismutase in the epidermis Q4 (author’s transl)”. Nippon Hifuka Gakkai Zasshi 92 (1982): 583-590.
  17. Luck H. “Methods of Enzymatic Analysis”. Verlag Chemie Academic Press. New York. USA (1963): 885-888.
  18. Sedlak J and Lindsay R. “Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent”. Analytical Biochemistry 25 (1968): 192-205.
  19. Szkudelski T. “The mechanism of alloxan and streptozotocin action in b-cells of the rat pancreas”. Physiology Research 50 (2001): 536-546.
  20. Dias A., et al. “Quercetin decreases oxidative stress, NF-B activation, and iNOS overexpression in liver of streptozotocin induced diabetic rats”. Journal of Nutrition 135 (2005): 2299-2304.
  21. Elbe H., et al. “Melatonin, quercetin and resveratrol attenuates oxidative hepatocellular injury in streptozotocin-induced diabetic rats”. Human and Experimental Toxicology9 (2015): 859-868.
  22. Hamadi N., et al. “Ameliorative effects of resveratrol on liver injury in streptozotocin-induced diabetic rats”. Journal of Biochemical and Molecular Toxicology 10 (2012): 384-392.
  23. Alam M., et al. “Protective effect of quercetin on hyperglycemia, oxidative stress and DNA damage in alloxan induced type 2 diabetic mice”. Life Science1 (2014): 8-14.
  24. Mohammad A., et al. “Neuroprotective effects of quercetin in diabetic rat retina”. Saudi Journal of Biological Sciences 24 (2017): 1186-1194.
  25. Arya A., et al. “Anti-diabetic effects of Centratherum anthelminticum seeds methanolic fraction on pancreatic cells, b-TC6 and its alleviating role in type 2 diabetic rats”. Journal of Ethnopharmacology 1 (2012): 22-32.
  26. Taha H., et al. “Upregulation of insulin secretion and downregulation of proinflammatory cytokines, oxidative stress and hyperglycemia in STZ nicotinamide- induced type 2 diabetic rats by Pseuduvaria Monticola bark extract”. Food and Chemical Toxicology 66 (2014): 295-306.
  27. Muragundla A and Kanwaljit C. “Quercetin, an anti- oxidant bioflavonoid, attenuates diabetic nephropathy in rats”. Clinical and Experimental Pharmacology and Physiology 31 (2004): 244-248.
  28. Abolfathi A., et al. “Protective effects of green tea extract against hepatic tissue injury in streptozotocin-induced diabetic rats”. Evidence-Based Complementary and Alternative Medicine (2012).
  29. Giuglino D., et al. “Diabetes mellitus, hypertension, and cardiovascular disease: which role for oxidative stress”. Metabolism3 (1995): 363-368.
  30. Babujanarthanam R., et al. “Quercitrin a bioflavonoid improves the antioxidant status in streptozotocin: induced diabetic rat tissues”. Molecular and Cellular Biochemistry 358 (2011): 121-129.
  31. Soheir NA and Suhailah SA. “Synergistics Effect of Quercetin Nanoparticles on Liver and Pancreas Tissues and Oxidative Stress of Streptozotocin-Induced Diabetic Rats”. Asian Journal of Chemistry5 (2017): 1003-1010.
  32. Coskun O., et al. “Quercetin, a flavonoid antioxidant, prevents and protects streptozotocin-induced oxidative stress and b-cell damage in rat pancreas”. Pharmacology Research2 (2005): 117-123.
  33. Gokmen K and Nurcan D. “The Effects of Quercetin on Antioxidant System and Some Blood Parameters in Experimental Diabetic Rats”. Bulletin of Environment, Pharmacology and Life Sciences 6 (2016): 28-32.
  34. Chan E., et al. “Relaxation to flavones and flavonols in rat isolated thoracic aorta: Mechanism of action and structure activity relationship”. Journal of Cardiovascular Pharmacology 35 (2000): 326-333.
  35. Robak J and Gryglewski R. “Flavonoids are scavengers of superoxide anions”. Biochemistry and Pharmacology 37 (1988): 837-841.
  36. Mustafa E., et al. “Quercetin, a powerful antioxidants bioflavonoid, attenuates renal dysfunction in long-term experimental diabetes mellitus”. Marmara Medical Journal2 (2011): 88-99.
  37. Molina M., et al. “Quercetin, a flavonoid antioxidant, prevents and protects against ethanol-induced oxidative stress in mouse liver”. Biological and Pharmaceutical Bulletin 10 (2003): 1398-1402.
  38. Adewole S., et al. “Projective effect of quercetin on the morphology of pancreatic β-cells of streptozotocin-treated diabetic rats”. African Journal of Traditional, Complementary and Alternative Medicines 1 (2007): 64-74.
  39. Çiftçi R and Yüce A. “Effect of Quercetin on Homocysteine Level and Coronary Vascular Damage in Rats with Liver Fibrosis”. Fırat University Journal of Health Sciences 3 (2013): 159-167.

Citation

Citation: Soheir N Abd El-Rahman., et al. “Effects of Quercetin Nanoparticles, An Antioxidant Bioflavonoid on the Retina of STZ-induced Diabetic Rats". Acta Scientific Women's Health 4.7 (2022): 65-73.

Copyright

Copyright: © 2022 Soheir N Abd El-Rahman., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




Metrics

Acceptance rate35%
Acceptance to publication20-30 days

Indexed In





News and Events


  • Certification for Review
    Acta Scientific certifies the Editors/reviewers for their review done towards the assigned articles of the respective journals.
  • Submission Timeline for Upcoming Issue
    The last date for submission of articles for regular Issues is July 30, 2024.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Article of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of "Best Article of the Issue"
  • Welcoming Article Submission
    Acta Scientific delightfully welcomes active researchers for submission of articles towards the upcoming issue of respective journals.

Contact US