Liandi Shen¹, Mengdi Niu², Yangyong Lu², Weihong Cao³ and Xueqiang Gao³*

¹Department of Maternal and Child Health, Jiading Maternal and Child Health Hospital, Shanghai, China
²Department of Breast and Thyroid, Qingdao Women and Children's Hospital, Qingdao, Shandong, China
³Department of Breast Disease Clinic, Qingdao University Hospital, Qingdao, Shandong, China

*Corresponding Author: Xueqiang Gao, Department of Breast Disease Clinic, Qingdao University Hospital, Qingdao, Shandong, China.

Received: July 30, 2021; Published: September 20, 2021

Abstract

  Short non-coding RNAs (microRNAs) is reported to exerted a crucial impact in tumor biology. While, the biological effect of miR-139 in breast cancer cells remain unclear yet. Here we intend to clarify the effect and mechanism of miR-139 derived from Bone marrow mesenchymal stem cells (BMSCs) on the biological behavior of gastric cancer cells. Breast cancer cells were divided into BMSC group (mixed culture of BMSC and breast cancer cells 1:1). Cells in the miR-139 mimics group, si-PXN group and control group were not treated. miR-139 abundance is evalued through Real time PCR, cell activity is analysed by MTT assay.Finally, the targeted binding of miR-139 to PXN was verified by double luciferase reporter genes. In relative to control, miR-139 abundance was notably declined in gastric cancer cells, while PXN abundance was higher, and the higher expression of PXN was linked to the prognosis of patients. miR-139 can be up-regulated by BMSCs or miR-139 mimics, thereby regulating EMT process through targeted inhibition of PCN, and ultimately inhibiting in vitro activity of breast cancer cells. BMSC co-culture can inhibit PCN by up-regulating miR-139, thus regulating EMT process and inhibiting the progression of gastric cancer. The results of this study revealed the mechanism of breast cancer progression to a certain extent, and suggested that miR-139 and PXN could be used as therapeutic targets for metastatic breast cancer, and BMSC could be of great value as a novel biologic therapy.

Keywords: Gastric Cancer; MiR – 139; Cell Proliferation; Cell Invasion

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Citation

Citation: Xueqiang Gao., et al. “Bone Marrow Mesenchymal Stem Cells (BMSC)-upregulated miR-139 Inhibited the Migration and Invasion of Breast Cancer Cells In Vitro by Inhibiting PXN Expression and EMT". Acta Scientific Women's Health 3.10 (2021): 03-10.

Copyright

Copyright: © 2021 Xueqiang Gao., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.