Evaluation of Mutation in Oxytocin Receptor Gene in rs35062132 Variant in Women with a History of Preterm Delivery and Women without a History of Preterm Delivery
Zahra Zahiri1*, Fatemeh Zahiri2 and Bolhasan Rezaei3
1Master of Molecular Genetics, Iran
2M.S. Student in Field of Genetics, Iran
3Ph.D of Molecular Genetics, Iran
*Corresponding Author: Zahra Zahiri, Master of Molecular Genetics, Iran.
May 03, 2021; Published: June 25, 2021
Background and Objective: Oxytocin is a neuropeptide made in the ventral and supraoptical adjacent nuclei of hypothalamus. Oxytocin is a hormone regarded for activity of the neuropeptides establishing sexual behaviors, but, first of all, it is known for a role played in establishment of contraction of uterus while delivering.
Receptor of oxytocin is OXTR and availability of its receptor depends on the maternal serum level which increase of serum plays an important role in progression of pregnancy. This research aims to study a relationship between rs35062132 (R376G) polymorphism of OXTR gene and/with premature labor.
Studying Method: In this study conducted by case control method in 2018, 152 women divided into two groups of those with premature labor history (from 22 to 36 weeks who referred to the Shahid Rajaei hospital of Tonekabon) and those without premature labor history were studied. After collection of information using questionnaire, sampling and performance of the rest steps, study of polymorphism was carried out by PCR-sequencing technique, and results of sequencing determination were analyzed by the Chromas software.
Finding: Out of collection of 152 studied cases, there existed rs35062132 polymorphism of OXTR gene in 53 individuals (34.8%) while presenting results of their sequencing determination and 99individuals (65.1%) lacked this polymorphism.
Conclusion: Results of this research show that there is a relationship between rs35062132 polymorphism of OXTR gene with premature labor.
Keywords: Oxytocin; Premature Labor; Polymorphism; PCR-sequencing
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