Khan Salman1*, Singh Priti2, Rashmi3, Zefenkey Zean4, Abdul Azeez Ibrahim4 and Salieva Rana5
1Assistant Professor, Department of Pathological Analysis, Knowledge University,
2International Medical Faculty, Osh State University, Osh, Kyrgyzstan
3Delhi Paramedical and Management Institute, New Delhi, India
4Department Pathological Analysis, Knowledge University, Erbil, Iraq
5Department of Pulmonary, Osh state university, Osh, Kyrgyzstan
*Corresponding Author: Khan Salman, Assistant Professor of Medical Microbiology and Immunology, Knowledge University, Erbil, Iraq.
Received: March 25, 2020; Published: April 01, 2020
Objective: Hepatitis B virus (HBV) infection during prenatal period presents with unique management issues for both the mother and fetus. These include the effects of HBV on maternal and fetal health, the effects of pregnancy on the course of HBV infection, treatment of HBV during pregnancy, and prevention of mother-to-child transmission.
Methods: Study conducted on 770 in clinically suspected pregnant female patients and performed in central research station laboratory of Microbiology at Netaji Subhash Chandra Bose Subharti Medical College in Meerut, India between November 2017 and February 2020. Serum samples were tested for HBsAg, HBeAg using ELISA.DNA was isolated and Amplified by RT-PCR by using pre-S gene and correlation with ALT, AST. Amplified DNA was Sequenced and genotyped by sequencer and analysed it on NCBI.
Results: 68 were positive for HBsAg in Pregnant female. 47 were HBeAg positive. 13 sample were positive for PCR. ALT was significant (P < 0.004) when correlate with DNA viral load. Genotype-B and genotype-A were 61.5% (n = 8), and 38.5% (n = 5) respectively.
Conclusion Genotype B was more prevalent in pregnancy. This study will improving current knowledge of genotype of hepatitis B in this region and will help clinicians to provide better therapy to the pregnant. For the differentiation of HBV infection level in diagnosis, HBsAg, HBeAg and ALT will be adequate in low income developing countries. Implementation of HBV-vaccine for both pre-exposure and post-exposure has been suggested to control HBV in Pregnant and new born baby. General population should get Vaccination from government hospitals to avoid this health problem.
Keywords: Hepatitis B Virus; Pregnancy; Genotypes; ALT; AST; RT-PCR
Citation: Khan Salman., et al. “Clinical Significance of Hepatitis B Virus-Genotypes and Correlation of HBV-DNA Viral Load with the Liver Enzyme in Pregnant Female”. Acta Scientific Women's Health 2.4 (2020): 21-26.
Copyright: © 2020 Khan Salman., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.