Acta Scientific Women's Health (ASWH)(ISSN: 2582-3205)

Research Article Volume 2 Issue 2

Inhibition Aggregation of Platelets with using Nanoparticles of Magnet-Controlled Sorbent (MCS-B) In Vitro Experiment

Andrey N Belousov*

Laboratory of Applied Nanotechnology of Belousov, Department of Anesthesio logy, Intensive Care, Transfusiology and Hematology, Kharkov Medical Academy of Postgraduate Education, Ukraine

*Corresponding Author: Andrey N Belousov, Laboratory of Applied Nanotechnology of Belousov, Department of Anesthesiology, Intensive Care, Transfusiology and Hematology, Kharkov Medical Academy of Postgraduate Education, Ukraine.

Received: December 16, 2019; Published: January 03, 2020

×

Abstract

  This scientific article is devoted to the study of the effect of magnetite nanoparticles (MCS-B brand) on the functional platelets activity of human. 0.9% NaCl was used as a control. The results of the study showed that the use of NaCl saline solution shifts the colloidal suspension equilibrium of platelets towards a significant (P<0.001) increase in the rate and index of their aggregation. For the first time, the effect of 0.9% NaCl on function of platelets makes us reconsider the concept of safety of infusion solutions in patients with initial signs of platelet disorders hemostasis. On the contrary, the use of MCS-B nanoparticles significantly revealed (P<0.05) an increase in the stability of colloidal suspension of platelets. This is an important pathogenetic factor which affects the occurrence of correction of hemostasis in conditions of blood clotting disorders.

Keywords: Platelets; Functional; MCS-B Nanoparticles; Saline Solution; Aggregation Rate; Aggregation Index

×

References

  1. Luzhnikov EA. Clinical toxicology. Medicine (1982): 368. 
  2. Zayko NN., et al. “Pathological physiology of the blood system”. In the book.: Pathological physiology. К.: "Logos". (1996): 125-130.
  3. Fundamentals of human physiology/Edited by B. I. Tkachenko - St. Petersburg: International foundation for the history of science 1 (1994): 566.
  4. Holodov YuA. “Magnetism in biology”. The science (1970): 96. 
  5. Zenkov NZ., et al. “The role of radical processes in the magnetosensitivity of the human body”. In the book: Magnetic fields in biology, medicine and agriculture. Abstracts of II Region of Science. Center for new information technologies. Rostov-on-Don (1985): 20-21. 
  6. Ponomarenko GN. “Electromagnetometry and phototherapy”. SPb.: Peace and family (1995): 159-166. 
  7. Jancovich BD., et al. “Magnetic hemotherapy as an effective resuscitation technique for treatment of acurte axogenic poisoning”. Current Toxicology 1 (1993): 133-139
  8. Lopuhin YuМ and Моlodenkov МI. “Hemosorption”. Меdicine (1982): 368. 
  9. Versin AA. “Magnetotherapy in ophthalmology”. In the book: Electromagnetic fields in the biosphere”. (2001): 65. 
  10. Mitbreit IM., et al. “Application of "running" pulsed low-frequency magnetic field in the treatment of patients with dystrophic diseases of the spine and joints”. In the book : Magnetic fields in biology, medicine and agriculture. Abstracts of the 2nd Scientific-Practical Conference-Rostov-on-don (1985): 91-93. 
  11. Surganova SF. “Magnetotherapeutic correction of ischemic disorders in extremities”. In the book: Magnetic fields in biology, medicine and agriculture. Abstracts of the 2nd Scientific-Practical Conference. Rostov-on-Don (1985): 127-128. 
  12. Smelova VI., et al. “Application of magnetotherapy in complex treatment of gynecological patients”. In the book: Magnetic fields in biology, medicine and agriculture. Abstracts of the 2nd Scientific-Practical Conference. Rostov-on-Don (1985): 196-198. 
  13. Orlov LL., et al. “The action of an impulse magnetic field in hypertension”. Soviet medicine (1987): 19-21.
  14. Kholodov YuA. Magnetism in biology. Moscow: Nauka (1970): 96.
  15. Novoselov VP., et al. “Some features of the influence of changes in Helio-geophysical factors on sudden death from coronary heart disease”. In the book: Magnetic fields in biology, medicine and agriculture. Abstracts of the 2nd Scientific-Practical Conference. Rostov-on-Don (1985): 122-123.
  16. Gvazava EА. “Evaluation of mutagenic activity of magnetic fluid of biological significance”. Abstracts of II Conferences "On the application of magnetic fluids in biology and medicine". Sukhumi (1985): 7-8.
  17. Frank VА. “The use of magnetically sensitive ointments in purulent surgery”. 5th All-Union meeting on the physics of magnetic fluids. Perm (1990): 115-116.
  18. Аhalaya МG., et al. “Obtaining and physical-chemical properties of magnetic fluids for biomedical applications”. Materials of the 3rd All-Union school-seminar on magnetic liquids. МSU (1983): 11-12.
  19. Permichev АN., et al. “Magnetically controlled hemosorption”. Abstracts of 7th international Pless conference on magnetic fluids. Application in biology and medicine. Pless (1996): 124-125.
  20. Belousov АN. Extracorporeal hemocorrection using magnetically controlled sorbent in intensive therapy of intoxication syndrome in patients with pathology of hepatopancreatoduodenal zone: Abstract of the dissertation for the degree of DM: 14.01.30. DSMA-Dnepropetrovsk (2004): 40.
  21. Belousov AN. Sorbent for extracorporeal detoxification of biological liquids: State Patent No. 24322A UA A61N2/00/Publ. 17.07.98.
  22. Belousov AN. Method of extracorporeal detoxification of biological fluids: State Patent No. 24183A UA A61N2/00/Publ. 17.07.98.
  23. Belousov AN. Method of production of a magnetic liquid for transport and retention of medicines in organism Patent of Ukraine №14817А UA A61N2/00/№ 96062463.
  24. Belousov AN. Therapeutic and prophylactic product "Micromage-B". State patent No. 30538A UA A 23L 1/304 No. 98052704 Decl. 25.05.98. Publ. 15.11.00.
  25. Belousov АN. “The use of magnetite nanoparticles in applied medicine”. International Journal of Nano Dimension 2.5 (2011): 25-28.
  26. Belousov AN and Belousova EYu. “The first steps in discovery new mechanisms of cellular regulation in means by nanotechnology preparations”. X International Conference New Information Technologies in Medicine and Ecology. Yalta, Gursuf (2002): 420-425.
  27. Belousov AN and Belousova EYu. Mechanisms cell regulation by nanotechnology preparations (MCS-B). BioNanotech Conference and Expo 2012. June 18-21, 2012, Santa Clura, CA, USA (2012).
  28. Belousov AN. “Effect of magnet controlled sorbent on parameters of acid-base balance of the blood and the processes of glycolysis in erythrocytes”. Pain, anesthesia and intensive care. Kiev (2000): 263-265.
  29. Belousov AN and Nevzorov VP. “Ultrastructure of cells in the kidneys and lungs of rabbits after administration of magnetite”. International collection of scientific papers IV Scientific and Practical Conference on the Creation and Testing of New Drugs. Moscow 4 (1997): 77-87.
  30. Belousov AN and Nevzorov VP. “Ultrastructure of liver cells after administration of magnetite”. International collection of scientific papers IV Scientific and Practical Conference on the Creation and Testing of New Drugs. Moscow 4 (1997): 71-77.
  31. Belousov AN. “Spectrum of Application Magnetite Nanopaticles in Medicine”. Nanotech 2 (2009): 154-157.
  32. Belousov AN. Effect on hemolysis and transport ATPase activity of erythrocytes by means nanopareticles of magnetit controlled sorbent (MCS-B). Pain, anesthesia and intensive care. Kiev. (2012): 26-28.
  33. Belousov AN. “Ultrastructure of Hepatic Cells after Intravenous Injection of Magnetite Nanoparticles ICNB”. Journal of Molecular Cell Biology 2.1 (2017).
  34. Belousov AN. “Nanotechnology and Discovery of a New Factor Which Influences on Permeability of Erythrocytes and Eryptosis”. Journal of Materials Science and Engineering A 4.11 (2014): 367-372.
  35. Belousov A., et al. “Influence of Physical Factors Caused by Magnetite Nanoparticles on Different Groups of Microorganisms”. Journal of Nanotechnology and Materials Science 2.2 (2015): 1-4.
  36. Order of the Ministry of health of the USSR No. 960 of 15.10.74 "on unification of clinical and laboratory research methods". A simplified means of determining platelet AG-regulation and disaggregation by born in the modification of Zachary, Kinah.
  37. Berkovsky AL., et al. “Manual on the increase of adhesive-aggregation activity of platelets” (2002): 28.
  38. Severina IS., et al. “Activation of soluble GU-anilate cyclase by new NO donors as a basis for directed search for new effective vasodilators and antiplatelets”. Bulletin of the Russian Academy of medical Sciences 4 (2000): 25-30.
  39. Chirkov Yu Yu., et al. “The Role of guanylate cyclase in the regulation of human platelet aggregation”. Bulletin of the USSR Academy of medical Sciences 10 (1991): 51-55.
  40. Belousov AN. Influence of a new method of extracorporeal hemocorrection with the use of a magnetically controlled sorbent on the blood circulation system in patients with intoxication syndrome.. Medicine today and tomorrow, Kharkiv state medical University (2000): 85-89.
  41. Movshovich IM and Shilo MA. “On the effect of MF on biological systems”. In the book: Proceedings of the 2nd All-Union meeting on the study of the influence of magnetic fields on biological objects. (1969): 155-158.
  42. Lezhenina NF and Rodionov VN. “The method of clinical toxometry in assessing the effectiveness of treatment of toxic encephalopathy in the somatogenic phase of acute poisoning. In the book: Theoretical and clinical issues of modern reanimatology progress”. Proceedings of the international Symposium (1999): 88.
  43. Klassen VI. “On the influence of weak magnetic fields on water systems. In the book: Reaction of biological systems to weak magnetic fields”. Proceedings of the All-Union Symposium (1971): 14-16.
  44. Kovalchuk AV. “On some mechanisms of action of PMF on biological objects. In the book: Reaction of biological systems to weak magnetic fields”. Proceedings of the All-Union Symposium (1971): 29-33.
  45. Luzhnikov EA and Goldfarb YS. “Physiohemotherapy of acute poisoning”. Medpraktika (2002): 199.
  46. Piccardi G. “L’influence des phenomenes terretres, solaires et cosmiques sur les reactions phisico-chimiques et biologiques”. Bruxelles (1956): 123.
  47. Straub KD and Carver P. “Effects of electromagnetic fields on microsomal ATPase and mitochondrial oxidative phosphorylation”. Annals of the New York Academy of Sciences 6 (1975): 247-254.
×

Citation

Citation: Andrey N Belousov. “Inhibition Aggregation of Platelets with using Nanoparticles of Magnet-Controlled Sorbent (MCS-B) In Vitro Experiment”. Acta Scientific Women's Health 2.2 (2020): 08-14.




Metrics

Acceptance rate35%
Acceptance to publication20-30 days

Indexed In





News and Events


  • Certification for Review
    Acta Scientific certifies the Editors/reviewers for their review done towards the assigned articles of the respective journals.
  • Submission Timeline for Upcoming Issue
    The last date for submission of articles for regular Issues is July 10, 2024.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Article of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of "Best Article of the Issue"
  • Welcoming Article Submission
    Acta Scientific delightfully welcomes active researchers for submission of articles towards the upcoming issue of respective journals.

Contact US