Acta Scientific Pharmaceutical Sciences (ASPS)(ISSN: 2581-5423)

Research Article Volume 5 Issue 8

Comparing the Effect of Bovine Albumin and Autologous Sera in Culture Medium (EmbryoCul-MHRM) in the Presence of Zn Supplementation on the Number of Embryos Produced by IVF in Mice

Mahdi Hadi1-3*, Fatemeh Ouroujzadeh4, Mahnaz Azarnia4 and Ghadireh Mirabolghasemi4

1Department of Reproductive Biology, Pishgam Paya zist Research and Knowledge Based Company, Tehran, Iran
2Department of Advanced Biological Technologies, Tamin Pharmaceutical Investment Company, Tofigh Darou Research and Engineering Company, Tehran, Iran
3Department of Embryology, Royan Research Institute, Reproductive Medicine Research Center, Tehran, Iran
4Department of Animal Biology, Kharazmi University, Faculty of Biological Sciences, Tehran, Iran

*Corresponding Author: Mahdi Hadi, Department of Reproductive Biology, Pishgam Paya zist Research and Knowledge Based Company, Tehran, Iran.

Received: April 12, 2021; Published: July 09, 2021

Abstract

Background: IVF techniques are developing as an effective treatment for the treatment of infertile couples. Accordingly, the present study designed to examine the effect of autologous serum and bovine serum albumin in the presence of zinc supplementation on the number of embryos produced.

Methods: 3 male and 1 female NMRI were used for the extraction of oocyte and sperm. Capacitate sperms were added to drops containing oocytes. The resulting compound was incubated for 4 - 6 hours in order to fertilization. Group design was performed in six groups: 1-control group, 2-control group + Zn (Zinc), 3-control group + AS (Autologous Serum), 4- AS + Zn, 5- AS (BSA free) and 6- free serum group with three replications in each group. After 24 and 48 hours, the 2 and 4 cells embryos were counted. Data were analyzed by ANOVA1. The difference between results in diverse groups was considered at P ≤ 0.05 level.

Results: The data from the experimental groups showed the percentage of embryos in the group containing AS was lower than the control group and the Zn group. The Zn group moreover had more embryos than the control group, but this increase was not significant. And according to the results, the presence of BSA is essential in the culture medium.

Conclusion: According to the results, it seems necessary to use of BSA in cultures, but its combination with AS is unrecommended. It is also advisable to use zinc supplements to increase the number of embryos in the culture medium.

Keywords: Autologous Serum; Bovine Serum Albumin; Zinc Supplement; IVF

References

  1. WHO I. A tabulation of available data on prevalence of primary and secondary infertility. Programme on Maternal and Child Health and Family Planning, Division of Family Health. World Health Organization, Geneva (1991).
  2. Steptoe PC. “Birth after the reimplantation of a human embryo”. Lancet 11 (1979): 366.
  3. Moussa M., et al. “Cryopreservation of mammalian oocytes and embryos: current problems and future perspectives”. Science China Life Sciences9 (2014): 903-914.
  4. Farquhar C and Marjoribanks J. “Assisted reproductive technology: an overview of Cochrane Reviews”. Cochrane Database of Systematic Reviews 8 (2018).
  5. Akhondi M., et al. “Ashnaei Ba Leghahe Tabiei, Leghah Khareje Rahemi Va Zarorat Estefade Az Gamet Jaygozin Dar Darmane Nabarvari”. SID Journal4 (2007): 307-321.
  6. Chronopoulou E and Harper JC. “IVF culture media: past, present and future”. Human Reproduction Update 1 (2014): 39-55.
  7. Rizos D., et al. “Bovine embryo culture in the presence or absence of serum: implications for blastocyst development, cryotolerance, and messenger RNA expression”. Biology of Reproduction1 (2003): 236-243.
  8. Hashemi Bani B and Razavi Sh. “Osol Va Mabaniye Keshte Selol Va Mohandesiye Baft”. First Edition. Esfahan: Isfahan University of Medical Sciences (2011): 55-56.
  9. Choi J., et al. “Effectiveness of autologous serum as an alternative to fetal bovine serum in adipose-derived stem cell engineering”. Cell and Tissue Banking3 (2013): 413-422.
  10. Favier AE. “The role of zinc in reproduction”. Biological Trace Element Research1-3 (1992): 363-382.
  11. Wani NA., et al. “Effect of different factors on the recovery rate of oocytes for in vitro maturation and in vitro fertilisation procedures in sheep”. Small Ruminant Research1 (1999): 71-76.
  12. Hafez ES and Hafez B. “Reproduction in farm animals”. 7th ed. south Carolina: John Wiley and Sons. (2013): 490-497.
  13. Ghadimi V and Zhandi M. “Asare albumine serume gavi bar keifiat sperm asbche khazar teye sardsai”. Iranian Journal of Animal Science2 (2015): 133-139.
  14. Gordon I. “Laboratory production of cattle embryos”. CABI1 (2003): 303-311.
  15. Varga S., et al. “Culture system and long-term storage of culture media in the in vitro production of bovine embryos”. Acta Veterinaria Hungarica 1 (2011): 129-139.
  16. Asad L., et al. “Effect of Bovine Serum Albumin on In vitro Maturation, In vitro Fertilization and Subsequent Development of Goat Embryos”. International Journal of Agriculture Innovations and Research 6 (2017).
  17. Rahman A., et al. “In vitro maturation and fertilization of buffalo oocytes cultured in media supplemented with bovine serum albumin”. IJAS3 (2015): 545-551.
  18. Hostetler CE., et al. “The role of essential trace elements in embryonic and fetal development in livestock”. The Veterinary Journal2 (2003): 125-139.
  19. Özkaya MO., et al. “Effects of multivitamin/mineral supplementation on trace element levels in serum and follicular fluid of women undergoing in vitro fertilization (IVF)”. Biological Trace Element Research1 (2011): 1-9.
  20. Bedwal RS and Bahuguna A. “Zinc, copper and selenium in reproduction”. Experientia 7 (1994): 626-640.
  21. Jeon Y., et al. “Supplementation of zinc on oocyte in vitro maturation improves preimplatation embryonic development in pigs”. Theriogenology 6 (2014): 866-874.
  22. Stephenson JL and Brackett BG. “Influences of zinc on fertilisation and development of bovine oocytes in vitro”. Zygote3 (1999): 195-201.
  23. Kincaid RL. “Assessment of trace mineral status of ruminants: A review”. Journal of Animal Science1 (1999): 1-10.
  24. Özkaya M., et al. “Effects of multivitamin/mineral supplementation on trace element levels in serum and follicular fluid of women undergoing in vitro fertilization (IVF)”. Biological Trace Element Research1 (2011): 1-9.
  25. Ghasemian F., et al. “The impact of different time intervals between hCG priming and oocyte retrieval on ART outcomes”. Iranian Journal of Reproductive Medicine7 (2013): 559.
  26. Tan JH., et al. “The impact of in vitro human embryo fragmentation on blastocyst development and ploidy using Next-Generation Sequencing (NGS)”. Reproductive BioMedicine Online1 (2019): e23.

Citation

Citation: Mahdi Hadi., et al. “Comparing the Effect of Bovine Albumin and Autologous Sera in Culture Medium (EmbryoCul-MHRM) in the Presence of Zn Supplementation on the Number of Embryos Produced by IVF in Mice". Acta Scientific Pharmaceutical Sciences 5.8 (2020): 03-09.

Copyright

Copyright: © 2020 Mahdi Hadi., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




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