Mahdi Hadi^1-3*, Fatemeh Ouroujzadeh⁴, Mahnaz Azarnia⁴ and Ghadireh Mirabolghasemi⁴

¹Department of Reproductive Biology, Pishgam Paya zist Research and Knowledge Based Company, Tehran, Iran
²Department of Advanced Biological Technologies, Tamin Pharmaceutical Investment Company, Tofigh Darou Research and Engineering Company, Tehran, Iran
³Department of Embryology, Royan Research Institute, Reproductive Medicine Research Center, Tehran, Iran
⁴Department of Animal Biology, Kharazmi University, Faculty of Biological Sciences, Tehran, Iran

*Corresponding Author: Mahdi Hadi, Department of Reproductive Biology, Pishgam Paya zist Research and Knowledge Based Company, Tehran, Iran.

Received: April 12, 2021; Published: July 09, 2021

Abstract

Background: IVF techniques are developing as an effective treatment for the treatment of infertile couples. Accordingly, the present study designed to examine the effect of autologous serum and bovine serum albumin in the presence of zinc supplementation on the number of embryos produced.

Methods: 3 male and 1 female NMRI were used for the extraction of oocyte and sperm. Capacitate sperms were added to drops containing oocytes. The resulting compound was incubated for 4 - 6 hours in order to fertilization. Group design was performed in six groups: 1-control group, 2-control group + Zn (Zinc), 3-control group + AS (Autologous Serum), 4- AS + Zn, 5- AS (BSA free) and 6- free serum group with three replications in each group. After 24 and 48 hours, the 2 and 4 cells embryos were counted. Data were analyzed by ANOVA1. The difference between results in diverse groups was considered at P ≤ 0.05 level.

Results: The data from the experimental groups showed the percentage of embryos in the group containing AS was lower than the control group and the Zn group. The Zn group moreover had more embryos than the control group, but this increase was not significant. And according to the results, the presence of BSA is essential in the culture medium.

Conclusion: According to the results, it seems necessary to use of BSA in cultures, but its combination with AS is unrecommended. It is also advisable to use zinc supplements to increase the number of embryos in the culture medium.

Keywords: Autologous Serum; Bovine Serum Albumin; Zinc Supplement; IVF

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Citation

Citation: Mahdi Hadi., et al. “Comparing the Effect of Bovine Albumin and Autologous Sera in Culture Medium (EmbryoCul-MHRM) in the Presence of Zn Supplementation on the Number of Embryos Produced by IVF in Mice". Acta Scientific Pharmaceutical Sciences 5.8 (2020): 03-09.

Copyright

Copyright: © 2020 Mahdi Hadi., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.