Manisai Koduri¹*, Pravallika Vataparthi², Nikhila Bolleda³, Vijaya Laxmi Golla³, Sravani Mereddy⁴, Sony Gunaganti¹

¹Former Assistant Professor, Department of Optometry, Centurion University of Technology and Management, Vizianagaram, Andhra Pradesh, India
²Assistant Professor, Department of Forensic Science, Centurion University of Technology and Management, Vizianagaram, Andhra Pradesh, India
³Bachelor Student, Department of Optometry, Centurion University of Technology and Management, Vizianagaram, Andhra Pradesh, India
⁴Assistant Professor and Head of the Department, Department of Optometry, Centurion University of Technology and Management, Vizianagaram, Andhra Pradesh, India

*Corresponding Author: Manisai Koduri, Former Assistant Professor, Department of Optometry, Centurion University of Technology and Management, Vizianagaram, Andhra Pradesh, India.

Received: February 13, 2023; Published: March 30, 2023

Abstract

Introduction: Identifying Barr bodies' presence has a significant diagnostic value in multifaceted science disciplines. Testing Barr bodies was important in diagnosing infertility, a syndromic association such as Klinefelter and psychopathic disorders, and disorders of sex development (DSD). It also plays a role in cancer detection in the uterine cervix, identifying transplanted retinal pigment epithelium in porcine models. Identifying the gender of victims or criminals becomes a fundamental requirement in any forensic analysis of a crime scene. The current study hypothesizes identifying the gender using Barr body detection from collected contact lens samples and tries to establish disposed soft contact lenses to consider as evidence found at the crime scene.

Methods: A total of 120 (60 males and 60 females) were included in the study; from each subject, contact lens and Buccal samples were collected using sterile wooden toothpicks and soft contact lenses after insertion and removal. Both the buccal and contact lens samples were built into two smears staining with Saffranine and Methylene blue stains. The smears underwent cytological assessment by two examiners using a binocular microscope at 40X. The details of findings were graded on a scale of 1 to 5 based on visualization of Barr bodies seen.

Results: The mean rank and median grading scores for higher using saffranine among females across both the samples. The sensitivity is higher at 100% for both the stains among contact lens samples, and specificity is higher among buccal samples, 93% for saffranine and 90% for methylene blue.

Conclusion: Overall, it is conspicuous that contact lenses can be considered as evidence found at the crime scene in identifying the gender using Barr body detection.

 Keywords: Barr Bodies; Contact Lens; Crime Scene; Saffranine; Sex Determination

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Citation

Citation: Manisai Koduri., et al. “Contact Lens as Evidence in Crime Scene - A Way to Identify Gender".Acta Scientific Ophthalmology 6.4 (2023): 50-58.

Copyright

Copyright: © 2023 Manisai Koduri., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.