Kuridze Nino¹, Gabunia Luiza¹, Ghambashidze Ketevan¹, Rtveladze Tekla¹, Iosebidze Tinatin², Gvishani Maia¹ and Vakhtang Shoshiashvili¹*

¹Tbilisi State Medical University, Tbilisi, Georgia
²Gori State University, Gori, Georgia

*Corresponding Author: Vakhtang Shoshiashvili, Tbilisi State Medical University, Tbilisi, Georgia.

Received: November 25, 2024; Published: December 16, 2024

Abstract

Skin wound healing is a complex and dynamic process essential for maintaining bodily integrity. This study investigates the impact of different therapeutic approaches (silicone, hypochlorous acid, heparin, and glucocorticoid-based preparations: Dermatix Ultra, Epicyn, Contractubex, Flosteron) on pro-inflammatory cytokines (IL-1, IL-6), healing progress and scar appearance in laboratory rats with full-thickness skin wounds. Dermatix Ultra, Contractubex, and Epicyn creams were administered 2–3 times daily, while Flosteron was given via subcutaneous injection weekly. Healing progress and scar appearance were evaluated using the Vancouver Scar Scale (VSS), and cytokine levels were measured by ELISA.

Results showed significant increases in IL-1 by day 7 across all groups. IL-1 levels normalized by day 21 in animals treated with Dermatix Ultra and Epicyn, while Flosteron-treated rats required until day 28. In contrast, Contractubex-treated and control groups exhibited persistent IL-1 elevation beyond day 28. Similarly, IL-6 peaked on day 14 but normalized by day 28 in Dermatix Ultra, Epicyn, and Flosteron groups, whereas it remained elevated in Contractubex and control groups.

Among the treatments, Dermatix Ultra emerged as the most effective, achieving superior scar characteristics, including reduced vascularity, improved pigmentation, pliability, and flattening. Epicyn and Flosteron demonstrated moderate efficacy, while Contractubex showed limited benefits in accelerating wound healing or improving scar quality.

Conclusion: These findings underscore the pivotal role of IL-1 and IL-6 in the inflammatory phase of wound healing and highlight the therapeutic potential of targeted treatments to modulate pro-inflammatory cytokines. Dermatix Ultra demonstrated the most robust outcomes, promoting rapid and effective healing, followed by Epicyn and Flosteron. The limited effectiveness of Contractubex suggests the need for further refinement in its application.

 Keywords: Skin Wounds; IL-1; IL-6; Dermatix Ultra; Epicyn; Flosteron; Contractubex

References

1. Marcia Ramos-e-Silva and Claudio de-Moura-Castro Jacques. “Epidermal barrier function and systemic diseases”. Clinics in Dermatology3 (2012): 277-279.
2. Proksch E., et al. “The skin: an indispensable barrier”. Experimental Dermatology12 (2008): 1063-1072.
3. Baroni A., et al. “Structure and function of the epidermis related to barrier properties”. Clinics in Dermatology3 (2012): 257-262.
4. Bayat A., et al. “Skin scarring”. BMJ 326 (2003): 88-92.
5. Martin P and Nunan R. "Cellular and molecular mechanisms of repair in acute and chronic wound healing". British Journal of Dermatology2 (2015): 370-378.
6. Eming SA., et al. “Inflammation in wound repair: molecular and cellular mechanisms". Journal of Investigation Dermatology9 (2014): 2350-2356.
7. Han G and Ceilley R. "Chronic wound healing: A review of current management and treatments”. Advances in Therapy 3 (2017): 599-610.
8. Gurtner GC., et al. “Wound repair and regeneration”. Nature 7193 (2008): 314-321.
9. Reinke J M and Sorg H. “Wound repair and regeneration”. European Surgical Research1 (2012): 35-43.
10. Dinarello CA. “Immunological and inflammatory functions of the interleukin-1 family”. Annual Review of Immunology 27 (2009): 519-550.
11. Gao J., et al. “Therapeutic potential of human mesenchymal stem cells producing IL-6 in a mouse melanoma lung metastasis model”. Stem Cells9 (2012): 1843-1854.
12. Wynn TA and Vannella KM. “Macrophages in tissue repair, regeneration, and fibrosis”. Immunity 3 (2016): 450-462.
13. Dunn LK and Schechter LN. “Management of hypertrophic scars and keloids”. Plastic and Reconstructive Surgery3 (2012): 447e-458e.
14. Huang L., et al. “Hypochlorous acid, an effective and safe alternative approach to control surgical site infections”. Frontiers in Microbiology 11 (2020): 737.
15. MacNeil S. “Progress and opportunities for tissue-engineered skin”. Nature 7130 (2007): 874-880.
16. Atiyeh Bishara S., et al. "Silicone gel sheeting for the treatment and prevention of hypertrophic scar: a meta-analysis of randomized controlled trials". International Wound Journal 3 (2017): 634-650.
17. Partsch H and Damstra R J. “Compression for leg wounds”. British Journal of Dermatology5 (2008): 947-948
18. Radford K. “Pain management in wound care”. British Journal of Nursing14 (2012): S4-S5.
19. Shih B and Garside E. “A systematic review of the effectiveness of silicone in hypertrophic scar and keloid management”. Journal of Cutaneous and Aesthetic Surgery3 (2014): 141-146.
20. Sidgwick G P., et al. “A comprehensive evidence-based review on the role of topicals and dressings in the management of skin scarring”. Archives of Dermatological Research6 (2015): 461-477.
21. Woo K Y and Coutts PM. “Advanced wound dressings”. Wound Healing and Skin Integrity: Principles and Practice 2 (2015): 102-118.
22. Zamboni WA., et al. “The effect of hyperbaric oxygen on revascularization of a rabbit ear chamber”. Plastic and Reconstructive Surgery 3 (2000): 990-996.
23. Park JW., et al. “Review of Scar Assessment Scales”. Medical Lasers 11 (2022): 1-7.
24. Fearmonti R., et al. “A review of scar scales and scar measuring devices”. Eplasty 10 (2010): 43.
25. Min Hui Choo A., et al. “Scar Assessment Tools: How Do They Compare?” Frontiers in Surgery (2021).
26. Rodrigues M., et al. “Wound healing: A cellular perspective”. Physiological Reviews1 (2019): 665-706.
27. Takeo M., et al. “Wound healing and skin regeneration”. Cold Spring Harbor Perspectives in Medicine1 (2015): a023267.
28. Dinarello CA. “Interleukin-1 in the pathogenesis and treatment of inflammatory diseases”. Blood 14 (2011): 3720-3732.
29. Lin ZQ., et al. “Essential involvement of IL-6 in the skin wound-healing process as evidenced by delayed wound healing in IL-6-deficient mice”. Journal of Leukocyte Biology 5 (2010): 863-875.

Citation

Citation: Vakhtang Shoshiashvili., et al. “Comparative Analysis of Different Methods of Treatment on Pro-Inflammatory Cytokines and the Skin Wound Healing Outcomes”.Acta Scientific Medical Sciences 9.1 (2025): 57-64.

Copyright

Copyright: © 2025 Vakhtang Shoshiashvili., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.