Jyoti*, Sachin Jain, Pankaj Kumar Umar, Vidhi Gautam, RK Sharma and Shashank Vishwakarma

Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science (NDVSU), Jabalpur, MP, India

*Corresponding Author: Jyoti, Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science (NDVSU), Jabalpur, MP, India.

Received: September 20, 2024; Published: November 29, 2024

Abstract

The present work was designed to evaluate antinociceptive activity of Annona squamosa in different pain models of zebrafish. Evaluation of anti-nociceptive activity of Annona squamosa was done in different pain models i.e. cinnamaldehyde and menthol induced nociception. A total of 90 zebra fish were taken and divided into 3 groups where each group except control (group 1) was divided into 4 subgroups i.e. A, B, C and D. The anti-nociceptive activity of Annona squamosa was determined by number of line crossing and behavioural response were also evaluated by novel tank diving test.

In cinnamaldehyde induced nociception, Annona squamosa significantly increased the number of line crossing from 71.3 ± 8.8 in cinnamaldehyde alone treated group to 102.1 ± 16.3 and 114.7 ± 18.5 in cinnamaldehyde + Annona squamosa @ 200 µg/ml treated group and cinnamaldehyde + Annona squamosa @ 400 µg/ml treated group, respectively. However, in menthol induced nociception, the effect of Annona squamosa was found to be non-significant.

The novel tank diving test revealed that Annona squamosa showed behavioural modification by significantly increasing the time spent in upper portion of tank, average entry duration as well as number of entries into upper portion of tank in comparision to cinnamaldehyde alone treated groups. However, Annona squamosa significantly decreased the other behavioural parameters like number of erratic movements, number of freezing bouts and freezing duration in comparision to cinnamaldehyde alone treated groups.

 Keywords: Annona squamosa; Zebrafish; Antinociceptive Activity

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Citation

Citation: Jyoti., et al. “Evaluation of Anti-Nociceptive Activity of Annona squamosa in Different Pain Models of Zebrafish”.Acta Scientific Medical Sciences 8.12 (2024): 195-203.

Copyright

Copyright: © 2024 Jyoti., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.