NM Kislitsyna¹*, AV Shatskikh¹, SM Dibirova¹ and SV Novikov²

¹The S. Fyodorov Eye Microsurgery Federal State Institution Beskudnikovsky Blvd, Moscow, Russia
²OOO “NEP MG” Beskudnikovsky Blvd, Moscow, Russia

*Corresponding Author: NM Kislitsyna, The S. Fyodorov Eye Microsurgery Federal State Institution Beskudnikovsky Blvd, Moscow, Russia.

Received: May 03, 2023; Published: October 13, 2023

Abstract

Purpose: The vitreous body (VB), due to the complexity of its structure, remains one of the least studied anatomical structures to this day. In literature there are attempts to describe the anatomy of the vitreous body, since the II century. The most relevant works are the studies of J. Worst., et al. in 1973, in which the authors proposed new methods of CT preparation with the introduction of dyes. Despite many years of research on the structure and functions of the vitreous body and the presence of a large number of works, and there are no methods and protocols for macromicroscopic examination of the vitreous body to develop a method and propose a protocol for macromicroscopic examination of the vitreous body (VB), allowing to obtain new data on VB topographic anatomy.

The purpose of the study is to develop the algorithm of examination of the vitreous body microsurgical anatomy that would let to assess its topographic specifics.

Materials and Methods: The proposed method of macromicroscopic examination was used to study the VB topographic anatomy of 38 cadaver eyeballs. In order to color transparent structures of the vitreous, poorly soluble metallic salts barium sulfate (Vitreocontrast) was used. Macroscopic examination was performed using a TopconOMS-800 operating microscope with a magnification of x8 to x21, microscopic changes were evaluated by light microscopy at x50, x100, x200, x400 x630 multiple magnification with Leica DM LB2 microscope followed by photographic recording. The algorithm for macroscopic examination performing.

Results and Discussion: The result of macroscopic preparation was the compilation of individual anatomical and topographic maps of VB patients. A distinctive feature of the developed method is the ability to dissect any VB structure and to isolate each cortical layer with the possibility of studying its anatomical and topographic features and relationships with underlying tissues (internal limiting membrane, ciliary body, lens capsule). In addition, the method allows to maintain the shape and integrity of the specimens after passing through all stages of histological processing. In order to fixate VB samples, we used a method with fixing VB structures on a special adhesive-metric tablet, and placing them in a biopsy bag placed in a biopsy cassette. After that, filled in formalin, the specimens were delivered to the laboratory, where all the stages of standard processing took place.

Conclusion: The developed technique of macromicroscopic examination of the vitreous allows to create an individual map of the VB topographic anatomy. After collecting of sufficient material and its statistical processing, it is possible to provide maps of the VB topographic anatomy in normal, age-related and pathological conditions.

 Keywords: Macromicroscopic Examination; Vitreous Body; Vitreoretinal Interface; "Vitreocontrast"; Vitreolenticular Interface

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Citation

Citation: NM Kislitsyna., et al. “Method for Vitreous Body Microsurgical Anatomy Studying”.Acta Scientific Medical Sciences 7.11 (2023): 52-62.

Copyright

Copyright: © 2023 NM Kislitsyna., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.