Acta Scientific Medical Sciences (ASMS)(ISSN: 2582-0931)

Research Article Volume 7 Issue 4

Bacteriological Assessment of Canned Drinks Surface

Iyevhobu KO1*, Babatope IO2, Ikede RE3, Omolumen LE4, Efosa KO5, Alleh AO6 and Omokpo VO7

1National Open University of Nigeria, Uromi Community Study Centre, Uromi, Edo State, Nigeria
2Department of Medical Laboratory Science, Faculty of Basic Medical Sciences, Ambrose Alli University, Ekpoma, Edo State, Nigeria
3Department of Bacteriology, Federal School of Medical Laboratory Technology, Nigeria
4Department of Chemical Pathology, Faculty of Medical Laboratory Science, Ambrose Alli University, Ekpoma, Edo State, Nigeria
5Centre for Biomedicine, Faculty of Health Sciences, University of Hull, United Kingdom
6Department of Public Health, Derby University, Kedleston Road, Derby, United Kingdom
7Department of Biological Sciences, School of Applied Science, Auchi Polytechnic Auchi, Edo State, Nigeria

*Corresponding Author: Iyevhobu KO, National Open University of Nigeria, Uromi Community Study Centre, Uromi, Edo State, Nigeria.

Received: February 09, 2023; Published: March 23, 2023

Abstract

Numerous types of food and beverages such as beer or soft drinks are commonly packaged in so-called "tin-cans". The adhesion and persistence of microorganisms to the surfaces can spread pathogens and spoilage microorganisms to foods, influencing their shelf-life and safety. Due to the fact that the bacteriology of the external orifice of canned drinks have not been extensively studied in various geographical locations and in this region, this study is limited to the investigation of the bacteria present on the external orifice of canned drinks in Ekpoma and to ascertain what group of bacteria the people of the locality consuming these drinks are possibly going to be exposed to. Ware houses and shops were randomly selected in Ekpoma to be used in this study. Forty (40) canned drinks towels were randomly used for this study from which twenty (20) were gotten from wholesalers (warehouses) and twenty (20) were gotten from retailers. Those from the retailers were divided into two (2) groups i.e. ten (10) from the refrigerator and ten (10) from unrefrigerated. Samples were taken by cotton swab which was scrubbed on the top surface of the canned drinks. Swabs were cultured on different Nutrient agar and incubated at 37°C and sub-cultured into relevant agars. Moreover, different biochemical tests were applied; catalase test, coagulase test and oxidase test. Microorganisms were recognized on the basis of macroscopic, microscopic and differential biochemical tests. The swabs were inoculated into phosphate buffer saline and incubated for a week before the swabs were streaked on various agar. Swabs were cultured on Nutrient agar, Blood agar, Saboraud dextrose agar (SDA) and Maconkey agar and incubated at 37°C. The different biochemical tests were applied; catalase test, Indole test, Oxidase test, Citrate test and Coagulase test. Microorganisms were identified on the basis of macroscopic, microscopic and differential tests. With regards to pathogenic bacteria, Staphylococcus aureus, Escherichia coli, Klebsiella pneumonia and Bacillus cereus was analyzed in about thirty-five (35) of the cans. With respect to the presence of microorganisms indicating general contamination, 35 out of 40 (87.5%) of the cans analyzed presented positive to aerobic microorganisms. With respect to contamination by fungi, 15 cans (37.5%) presented positive to Aspergilus spp and Candida albicans.

 Keywords: Canned Drinks; Orifice Staphylococcus aureus; Escherichia coli; Klebsiella pneumonia and Bacillus cereus

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Citation

Citation: Iyevhobu KO., et al. “The Neonates' Body Length and Body Weight in Two Different Periods of Time”.Acta Scientific Medical Sciences 7.4 (2023): 159-164.

Copyright

Copyright: © 2022 Iyevhobu KO., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




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Impact Factor1.403

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