Maria Concetta D’Ovidio¹*, Pasquale Capone¹, Andrea Lancia², Paola Melis¹, Ornella De Pità³, Elvira Macale³, Francesca dé Donato⁴, Manuela De Sario⁴, Paola Michelozzi⁴, Stefania Massari⁵, Annarita Wirz⁶, Roberto Moccaldi⁷, Carlo Grandi¹ and Renato Ariano⁸

¹Department of Occupational and Environmental Medicine, Epidemiology and Hygiene, Italian Workers’ Compensation Authority (INAIL), Monte Porzio Catone, Rome, Italy
²Department of Environmental Biology, Sapienza University of Rome, Rome, Italy
³Clinical Pathology Laboratory, Cristo Re Hospital, Roma, Rome, Italy
⁴Department of Epidemiology of the Regional Health Service, ASL Roma 1, Rome, Italy
⁵Department of Occupational and Environmental Medicine, Epidemiology and Hygiene, Italian Workers’ Compensation Authority (INAIL), Rome, Italy
⁶Santa Lucia Foundation, Rome, Italy
⁷Prevention and Protection Unit, National Research Council (CNR), Rome, Italy
⁸Association of Italian Territorial and Hospital Allergologists and Immunologists (AAIITO), Florence, Italy

*Corresponding Author: Maria Concetta D’Ovidio, Department of Occupational and Environmental Medicine, Epidemiology and Hygiene, Italian Workers’ Compensation Authority (INAIL), Monte Porzio Catone (Rome), Italy.

Received: November 14, 2022; Published: December 05, 2022

Abstract

Background: The air quality guidelines published by WHO in 2021 underlines the need to study multipollutant exposures to investigate additive, synergistic or antagonistic effects of air pollutants including pollens. An important research area concerns the study of action mechanisms and the advanced methodologies for the evaluation of such exposure.

Multiplex methodologies are important tools that can improve the study of health responses followed exposure to agents from different sources and in particular in the research involving allergy. The use of multiplex methods can define the individual allergic profile, addressing the investigation of the action mechanisms and the identification of new biomarkers.

The aim of this paper is to promote the use of innovative methodologies applied to occupational allergies, showing the results obtained with two multiplex systems.

Methods: A total of 43 workers enrolled in an animal research facility and 105 patients were screened with two multiplex methods (ImmunoCap ISAC and ALEX) to evaluate allergic reactivity towards about 100-300 allergens.

Results: The results obtained with ImmunoCap ISAC evidenced a higher reactivity for plant allergens, while ALEX methodology showed that the pollens were the allergens with the higher IgE values.

These results highlight the role of plant allergens, in particular pollen, able to interact with chemical agents, as well as with several factors such as microclimate and meteorological variables. Pollen exposure is also affected by the ongoing climate change as well as by anthropic factors such as occupants in indoor settings.

Conclusion: The multiplex methodologies may be applied on biological and environmental matrices to improve the knowledge of the action mechanisms of allergies. The identification of novel biomarkers using multiplex methodologies is a further opportunity in the study of occupational allergies.

Keywords: Allergens; Occupational Allergy; Multiplex Methodologies; Occupational Health; Plant Allergens; Animal Allergens

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Citation

Citation: Maria Concetta D’Ovidio., et al. “Multiplex Methodologies in the Study of Occupational Allergies Due to Animal and Plant-derived Allergens Exposure”.Acta Scientific Medical Sciences 7.1 (2023): 02-08.

Copyright

Copyright: © 2022 Maria Concetta D’Ovidio., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.