Acta Scientific Medical Sciences (ASMS)(ISSN: 2582-0931)

Research Article Volume 7 Issue 1

Multiplex Methodologies in the Study of Occupational Allergies Due to Animal and Plant-derived Allergens Exposure

Maria Concetta D’Ovidio1*, Pasquale Capone1, Andrea Lancia2, Paola Melis1, Ornella De Pità3, Elvira Macale3, Francesca dé Donato4, Manuela De Sario4, Paola Michelozzi4, Stefania Massari5, Annarita Wirz6, Roberto Moccaldi7, Carlo Grandi1 and Renato Ariano8

1Department of Occupational and Environmental Medicine, Epidemiology and Hygiene, Italian Workers’ Compensation Authority (INAIL), Monte Porzio Catone, Rome, Italy
2Department of Environmental Biology, Sapienza University of Rome, Rome, Italy
3Clinical Pathology Laboratory, Cristo Re Hospital, Roma, Rome, Italy
4Department of Epidemiology of the Regional Health Service, ASL Roma 1, Rome, Italy
5Department of Occupational and Environmental Medicine, Epidemiology and Hygiene, Italian Workers’ Compensation Authority (INAIL), Rome, Italy
6Santa Lucia Foundation, Rome, Italy
7Prevention and Protection Unit, National Research Council (CNR), Rome, Italy
8Association of Italian Territorial and Hospital Allergologists and Immunologists (AAIITO), Florence, Italy

*Corresponding Author: Maria Concetta D’Ovidio, Department of Occupational and Environmental Medicine, Epidemiology and Hygiene, Italian Workers’ Compensation Authority (INAIL), Monte Porzio Catone (Rome), Italy.

Received: November 14, 2022; Published: December 05, 2022

Abstract

Background: The air quality guidelines published by WHO in 2021 underlines the need to study multipollutant exposures to investigate additive, synergistic or antagonistic effects of air pollutants including pollens. An important research area concerns the study of action mechanisms and the advanced methodologies for the evaluation of such exposure.

Multiplex methodologies are important tools that can improve the study of health responses followed exposure to agents from different sources and in particular in the research involving allergy. The use of multiplex methods can define the individual allergic profile, addressing the investigation of the action mechanisms and the identification of new biomarkers.

The aim of this paper is to promote the use of innovative methodologies applied to occupational allergies, showing the results obtained with two multiplex systems.

Methods: A total of 43 workers enrolled in an animal research facility and 105 patients were screened with two multiplex methods (ImmunoCap ISAC and ALEX) to evaluate allergic reactivity towards about 100-300 allergens.

Results: The results obtained with ImmunoCap ISAC evidenced a higher reactivity for plant allergens, while ALEX methodology showed that the pollens were the allergens with the higher IgE values.

These results highlight the role of plant allergens, in particular pollen, able to interact with chemical agents, as well as with several factors such as microclimate and meteorological variables. Pollen exposure is also affected by the ongoing climate change as well as by anthropic factors such as occupants in indoor settings.

Conclusion: The multiplex methodologies may be applied on biological and environmental matrices to improve the knowledge of the action mechanisms of allergies. The identification of novel biomarkers using multiplex methodologies is a further opportunity in the study of occupational allergies.

Keywords: Allergens; Occupational Allergy; Multiplex Methodologies; Occupational Health; Plant Allergens; Animal Allergens

References

  1. World Allergy Organization (WAO). “WAO White Book on Allergy 2013 Update”. Ed by R. Pawankar, S.T. Holgate, G.W. Canonica, R.F. Lockey, M.S. Blaiss (2013).
  2. European Academy of Allergy and Clinical Immunology (EAACI). “Global Atlas of Asthma”. Ed by I. Agache, A. Cezmi, Akdis 2nd Edition. April (2021).
  3. National Institute for Occupational Safety and Health (NIOSH). “Work-related asthma”.
  4. Quirce S and Sastre J. “Sensitizing agents inducers of occupational asthma, hypersensitivity pneumonitis and eosinophilic bronchitis”. World Allergy Organization (WAO) (2019).
  5. Commission des normes, de l'équité, de la santé et de la sécurité du travail (CNESST). “List of agents causing occupational asthma” (2022).
  6. Sigsgaard T., et al. “Respiratory diseases and allergy in farmers working with livestock: a EAACI position paper”. Clinical and Translational Allergy29 (2020): 29.
  7. Simoneti CS., et al. “Allergic sensitization to laboratory animals is more associated with asthma, rhinitis, and skin symptoms than sensitization to common allergens”. Clinical and Experimental Allergy11 (2017): 1436-1444.
  8. D’Ovidio MC., et al. “Biological occupational allergy: protein microarray for the study of laboratory animal allergy (LAA)”. AIMS Public Health4 (2018): 352-365.
  9. Sparkes AH. “Human allergy to cats: A review for veterinarians on prevalence, causes, symptoms and control”. Journal of Feline Medicine and Surgery1 (2022): 31-42.
  10. Jeebhay MF., et al. “Food processing and occupational respiratory allergy. An EAACI position paper”. Allergy10 (2019): 1852-1871.
  11. Jeebhay MF and Baatjies R. “Occupational inhalant allergy in food handling occupations”. Current Opinion in Allergy and Clinical Immunology 2 (2022): 64-72.
  12. Mwanga HH., et al. “Work-related allergy and asthma associated with cleaning agents in health workers in Southern African tertiary hospitals”. American Journal of Industrial Medicine5 (2022): 382-395.
  13. Huntley CC., et al. “Occupational asthma in office workers”. Occupational Medicine (Lond) 72.6 (2022): 411-414.
  14. Vlahovich KP and Sood A. “A 2019 update on occupational lung diseases: a narrative review”. Pulmonary Therapy1 (2021): 75-87.
  15. Tizek L., et al. “Urban vs rural - Prevalence of self-reported allergies in various occupational and regional settings”. World Allergy Organization Journal1 (2022): 100625.
  16. Kube H., et al. “From workplace to home environment: spreading of mouse allergens by laboratory animal workers”. International Archives of Occupational and Environmental Health4 (2021): 601-610.
  17. Sander I., et al. “Comparing the concentration levels of allergens and endotoxins in employees’ homes and offices”. International Archives of Occupational and Environmental Health3 (2022): 573-588.
  18. Zahradnik E., et al. “Animal allergens, endotoxin, and β- (1,3)-glucan in small animal practices: exposure levels at work and in homes of veterinary staff”. Annals of Work Exposures and Health 66 (2022): 27-40.
  19. Zahradnik E and Raulf M. “Animal allergens and their presence in the environment”. Frontiers in Immunology 5 (2014): 76.
  20. Tafuro F., et al. “Work-related allergies to storage mites in Parma (Italy) ham workers”. BMJ Open5 (2015): e007502.
  21. Stefanovic N., et al. “The role of the environment and exposome in atopic dermatitis”. Current Treatment Options in Allergy3 (2021): 222-241.
  22. Harada K and Miller RL. “Environmental exposures: evolving evidence for their roles in adult allergic disorders”. Current Opinion in Allergy and Clinical Immunology1 (2022): 24-28.
  23. Xing Y., et al. “Environmental influences and allergic diseases in the asia-pacific region: what will happen in next 30 years?” Allergy, Asthma & Clinical Immunology1 (2022): 21-39.
  24. Pacheco SE., et al. “Climate change and global issues in allergy and immunology”. Journal of Allergy and Clinical Immunology 6 (2021): 1366-1377.
  25. Rothenberg ME. “Linking the allergy epidemic to climate change”. Nature Immunology2 (2022): 149.
  26. Shankar HM and Rice MB. “Update on climate change: its impact on respiratory health at work, home, and at play”. Clinics in Chest Medicine4 (2020): 753-761.
  27. Rodríguez-Fernández A., et al. “A case of study in central northwest of Spain: how to select the optimal monitoring locations for an aerobiological network”. Science of the Total Environment 827 (2022): 154370.
  28. Wang, et al. “Influence of various factors on indoor/outdoor pollen concentration ratio based on experimental research: a review”. Building and Environment 219.11 (2022): 109154.
  29. Pawankar R., et al. “Asia Pacific Association of Allergy Asthma and Clinical Immunology White Paper 2020 on climate change, air pollution, and biodiversity in Asia-Pacific and impact on allergic diseases”. Asia Pacific Allergy1 (2020): e11.
  30. Choi YJ., et al. “The impact of climate change on pollen season and allergic sensitization to pollens”. Immunology and Allergy Clinics of North America1 (2021): 97-109.
  31. Plaz MP and Alcázar P. “Atmospheric pollutants and their association with olive and grass aeroallergen concentrations in Córdoba (Spain)”. Environmental Science and Pollution Research36 (2020): 45447-45459.
  32. D’Ovidio MC., et al. “Pollen and fungal spores evaluation in relation to occupants and microclimate in indoor workplaces”. Sustainability6 (2021): 3154.
  33. Lancia A., et al. “Research progress on aerobiology in the last 30 years: a focus on methodology and occupational health”. Sustainability8 (2021): 4337.
  34. Pelliccioni A., et al. “Intercomparison of indoor and outdoor pollen concentrations in rural and suburban research workplaces”. Sustainability16 (2021): 8776.
  35. D’Ovidio MC., et al. “Climate change and occupational allergies: an overview on biological pollution, exposure and prevention”. Annali dell'Istituto Superiore di Sanità3 (2016): 406-414.
  36. Sedghy F and Varasteh AR. “Interaction between air pollutants and pollen grains: the role on the rising trend in allergy”. Reports of Biochemistry and Molecular Biology2 (2018): 219-224.
  37. Kolek F and Plaza MDP. “Earlier flowering of Betula pendula roth in Augsburg, Germany, due to higher temperature, NO2 and urbanity, and relationship with Betula pollen season”. International Journal of Environmental Research and Public Health 18.19 (2021): 10325.
  38. D’Amato G., et al. “The effects of climate change on respiratory allergy andasthma induced by pollen and mold allergens”. Allergy 9 (2020): 2219-2228.
  39. World Health Organization (WHO). “Health and the environment”. 4 April (2022).
  40. National Institute for Occupational Safety and Health (NIOSH). “Preventing asthma in animal handlers. NIOSH Alert”. DHHS (NIOSH) Publication No. 97-116. January (1998).
  41. Cockcroft A., et al. “Allergy in laboratory animal workers”. Lancet8224 (1981): 827-830.
  42. Agrup G., et al. “Allergy to laboratory animals in laboratory technicians and animal keepers”. British Journal of Industrial Medicine 43 (1986): 192-198.
  43. Bush RK and Stave GM. “Laboratory animal allergy: an update”. ILAR Journal1 (2003): 28-51.
  44. Acton D and McCauley L. “Laboratory animal allergy. An occupational hazard”. AAOHN Journal6 (2007): 241-244.
  45. Zahradnik E and Raulf M. “Respiratory allergens from furred mammals: environmental and occupational exposure”. Veterinary Sciences3 (2017): 38.
  46. Simoneti CS., et al. “Allergic sensitization to laboratory animals is more associated with asthma, rhinitis, and skin symptoms than sensitization to common allergens”. Clinical and Experimental Allergy 1 (2017): 1436-1444.
  47. Stave GM. “Occupational animal allergy”. Current Allergy and Asthma Reports2 (2018): 11.
  48. Kang SY., et al. “Prevalence and diagnostic values of laboratory animal allergy among research personnel”. Asian Pacific Journal of Allergy and Immunology Jul 11. (2021).
  49. Watt AD and McSharry CP. “Laboratory animal allergy: anaphylaxis from a needle injury”. Occupational and Environmental Medicine8 (1996): 573-574.
  50. Kampitak T and Betschel SD. “Anaphylaxis in laboratory workers because of rodent handling: two case reports”. Journal of Occupational Health4 (2016): 381-383.
  51. World Health Organization (WHO). “WHO global air quality guidelines. Particulate matter (PM2.5 and PM10), ozone, nitrogen dioxide, sulfur dioxide and carbon monoxide”. Geneva: World Health Organization, 2021. Licence: CC BY-NC-SA 3.0 IGO.
  52. Donovan BM and Bastarache L. “The current state of omics technologies in the clinical management of asthma and allergic diseases”. Annals of Allergy, Asthma and Immunology6 (2019): 550-557.
  53. Raulf M., et al. “Addressing molecular diagnosis of occupational allergies”. Current Allergy and Asthma Reports 1 (2018): 6.
  54. Quan PL., et al. “Validation of a commercial allergen microarray platform for specific immunoglobulin E detection of respiratory and plant food allergens”. Annals of Allergy, Asthma and Immunology 128 (2022): 283-290.e4.
  55. Directive 2001/20/EC of the European Parliament and of the Council of 4 April 2001 on the Approximation of the Laws, Regulations and Administrative Provisions of the Member States Relating to the Implementation of Good Clinical Practice in the Conduct of Clinical Trials on Medicinal Products for Human Use (2001).
  56. International Commission on Occupational Health (ICOH). “International Code of Ethics for Occupational Health Professionals”. ICOH: Mumbai, India (2014).
  57. Cissé G., et al. “Health, Wellbeing, and the Changing Structure of Communities”. In: Climate Change 2022: Impacts, Adaptation and Vulnerability. Contribution of Working Group II to the Sixth Assessment Report of the Intergovernmental Panel on Climate Change. Edited by H.O. Pörtner, D.C. Roberts, M. Tignor, E.S. Poloczanska, K. Mintenbeck, A. Alegría, M. Craig, S. Langsdorf, S. Löschke, V. Möller, A. Okem, B. Rama. Cambridge University Press, Cambridge, UK and New York, NY, USA (2022): 1041-1170.
  58. Ogulur, I., et al. “Advances and highlights in biomarkers of allergic diseases”. Allergy12 (2021): 3659-3686.

Citation

Citation: Maria Concetta D’Ovidio., et al. “Multiplex Methodologies in the Study of Occupational Allergies Due to Animal and Plant-derived Allergens Exposure”.Acta Scientific Medical Sciences 7.1 (2023): 02-08.

Copyright

Copyright: © 2022 Maria Concetta D’Ovidio., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




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