Protective Effect of Arabian Balsam (Commiphora gileadensis) Against Hepatorenal Toxicity of Diazinon in Male Rats
Mohsen Ali Khormi1*, Moustafa Hussein Roshdy Elnaggar2,3 and Mansour Attia Al-Hazmi2
1Department of Biological Sciences, Faculty of Sciences, Jazan University, KSA
2Department of Biological Sciences, Faculty of Sciences, King Abdulaziz University, KSA
3Department of Zoology, Faculty of Sciences, Suez Canal University, Ismailia, Egypt
*Corresponding Author: Mohsen Ali Khormi, Department of Biological Sciences, Faculty of Sciences, Jazan University, KSA.
April 14, 2022; Published: May 09, 2022
The aim of the present study was to assess the protective effect of the Arabian Balsam (Commiphora gileadensis) against the hepatorenal toxicity of Diazinon (DZN) on adult male albino rats. Forty male albino rats weighing 180-220 g were divided into four equal groups as follows: Group I (control group): untreated group that received 0.2 mg/kg of corn oil, daily for 6 weeks. Group II (DZN group): orally administrated 50 mg/kg of DZN in corn oil, daily for 6 weeks. Group III (Balsam group): orally administrated Arabian balsam 500 mg/kg daily for 6 weeks. Group IV (Balsam + DZN group): orally administrated 500 mg/kg balsam + 50 mg/kg DZN, daily for 6 weeks. After six weeks, blood samples were collected from retro-orbital venous plexus. Serum samples used to determine levels of liver and kidney functions. After blood sampling, livers and kidneys were isolated and ﬁxed in 10% buffered formalin and then were processed and stained with Hematoxylin and Eosin stain and examined under light microscope. DZN administration caused significant decrease in final body weights, total proteins, albumin and high density lipoprotein cholesterol but significant increase in triglyceride, total cholesterol, low density lipoprotein cholesterol, very low density lipoprotein cholesterol, aspartate amino-transferase, alanine, aminotransferase, alkaline phosphatase, gamma glutamyl transferase, creatinine and urea nitrogen, creatinine kinase and lactate dehydrogenase enzymes compared with control and Balsam treated groups. Balsam administration led to significant decrease in blood glucose level versus control and DZN groups. In balsam + DZN group led to improvement of lipid profile, liver function tests, kidney function tests and tissue destructive enzymes. These improvements were confirmed histologically. In conclusion, DZN caused hepatorenal toxicity in rats that was improved by Arabian Balsam administration for 6 weeks. In conclusion, DZN led to hyperglycemia, dyslipidemia, alteration of liver and kidney function tests increased in tissue destructive markers as CK and LDH. Administration of balsam led to improvement of measured parameters. These improvements were confirmed histologically. In conclusion, DZN caused hepatorenal toxicity in rats that was improved by Arabian Balsam administration for 6 weeks.
Keywords: Arabian Balsam; Commiphora gileadensis; Diazinon; Kidney; Liver; Physiology; Histology; Rats
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