Acta Scientific Medical Sciences (ASMS)(ISSN: 2582-0931)

Research Article Volume 6 Issue 6

Protective Effects of Manuka Honey Against Diazinon Induced Hepatorenal Toxicity in Adult Male Rats

Mohsen Ali Khormi1*, Moustafa Hussein Roshdy Elnaggar2,3 and Mansour Attia Al-Hazmi2

1Department of Biological Sciences, Faculty of Sciences, Jazan University, KSA
2Department of Biological Sciences, Faculty of Sciences, King Abdulaziz University, KSA
3Department of Zoology, Faculty of Sciences, Suez Canal University, Ismailia, Egypt

*Corresponding Author: Mohsen Ali Khormi, Department of Biological Sciences, Faculty of Sciences, Jazan University, KSA.

Received: April 14, 2022; Published: May 03, 2022

Abstract

Manuka honey is mono-floral honey produced by Manuka myrtle tree. MH has anti-inflammatory, anti-bacterial, anticancer, wound healing and other benefits. This experimental study aimed to monitor Diazinon administration effects on liver and kidney of adult male Albino rats and protective value of 6 weeks administration of MH against effects of Diazinon and mechanism of their actions. Forty rats weighing 180-220 g divided into four groups (10 rats each): Group I (control group): rats received 0.2 mg/kg corn oil, daily for 6 weeks. Group II (DZN group): rats orally administrated 50 mg/kg DZN in corn oil, daily for 6 weeks. Group III (MH group): rats orally administrated 1.5 g/kg MH daily for 6 weeks. Group IV (DZN + MH group): rats orally administrated 50 mg/kg DZN and 1.5 g/kg MH, daily for 6 weeks. After six weeks, blood samples collected from retro-orbital venous plexus. Serum samples used to determine levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), gamma-glutamyl transferase (GGT), total bilirubin, creatinine, blood urea nitrogen (BUN), total protein (TP), albumin (ALB), glucose, triglycerides (TG), total cholesterol (CHO), high-density lipoprotein cholesterol (HDL-c), low-density lipoprotein cholesterol (LDL-c) very-low-density lipoprotein cholesterol (VLDL-c), creatinine kinase (CK) and lactate dehydrogenase (LDH). After blood sampling, livers and kidneys isolated, fixed in 10% buffered formalin and examined under light microscope. DZN led to hyperglycemia, increased in serum levels of triglyceride, CHO, LDL-C, VLDL-C, AST, ALT, ALP, GGT, creatinine, Blood urea nitrogen, CK and LDH but significant decreased in total proteins and albumin. Administration of MH led to improvement of blood glucose, lipid profile, liver function tests and kidney function tests, Tissue destructive enzymes. These improvements were confirmed histologically by examination of hepatic and renal tissues. In conclusions, DZN had hepatotoxicity and renal toxicity that improved by Manuka honey administration for 6 weeks.

Keywords: Manuka Honey; Diazinon; Physiology; Histology; Kidney; Liver; Rats

References

  1. Mojiri A., et al. “Pesticides in aquatic environments and their removal by adsorption methods”. Chemosphere 253 (2020):
  2. Sidhu GK., et al. “Toxicity, monitoring and biodegradation of organophosphate pesticides: a review”. Critical Reviews in Environmental Science and Technology 49.13 (2019): 1135-1187.
  3. Liu J., et al. “Alterations of antioxidant indexes and inflammatory cytokine expression aggravated hepatocellular apoptosis through mitochondrial and death receptor-dependent pathways in Gallus gallus exposed to arsenic and copper”. Environmental Science and Pollution Research16 (2018): 15462-15473.
  4. Nili-Ahmadabadi A., et al. “The role of ghrelin and tumor necrosis factor alpha in diazinon-induced dyslipidemia: insights into energy balance regulation”. Pesticide Biochemistry and Physiology 157 (2019): 138-142.
  5. Ueyama J., et al. “Effect of the organophosphorus pesticide diazinon on glucose tolerance in type 2 diabetic rats”. Toxicology Letters 182.1-3 (2008): 42-47.
  6. Gamhewage M., et al. “Phenoxy herbicides’ interactions with river bottom sediments”. Journal of Soils and Sediments 19.10 (2019): 3620-3630.
  7. El-Senduny FF., et al. “Manuka honey, a unique mono-floral honey. A comprehensive review of its bioactives, metabolism, action mechanisms, and therapeutic merits”. Food Bioscience (2021): 101038.
  8. Schuhladen K., et al. “Manuka honey and bioactive glass impart methylcellulose foams with antibacterial effects for wound-healing applications”. Biomedical Materials 15.6 (2020): 065002.
  9. Al-Attar AM and Zari TA. “Influences of crude extract of tea leaves, Camellia sinensis, on streptozotocin diabetic male albino mice”. Saudi Journal of Biological Sciences 17.4 (2010): 295-301.
  10. Trinder P. “Colorimetric method for glucose determination in blood and other biological fluids”. A Annals of Clinical Biochemistry 6 (1969):
  11. Fossati P and Prencipe L. “Serum triglycerides determined colorimetrically with an enzyme that produces hydrogen peroxide”. Clinical Chemistry 28.10 (1982): 2077-2080.
  12. Richmond W. “Preparation and properties of a cholesterol oxidase from Nocardia sp. and its application to the enzymatic assay of total cholesterol in serum”. Clinical Chemistry. 19.12 (1973): 1350-1356.
  13. Warnick GR., et al. “Estimation of HDL-cholesterol selected methods”. Clinical Chemistry 1 (1983): 91-99.
  14. Friedewald WT., et al. “Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge”. Clinical Chemistry 18.6 (1972): 499-502.
  15. Reitman S and Frankel S. “A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases”. American Journal of Clinical Pathology 28.1 (1957): 56-63.
  16. McComb RB and Bowers GN. “Study of optimum buffer conditions for measuring alkaline phosphatase activity in human serum”. Clinical Chemistry 18.2 (1972): 97-104.
  17. Szasz G. “A kinetic photometric method for serum γ-glutamyl transpeptidase”. Clinical Chemistry 15.2 (1969): 124-136.
  18. Doumas BT., et al. “Standardization in bilirubin assays: evaluation of selected methods and stability of bilirubin solutions”. Clinical Chemistry 19.9 (1973): 984-993.
  19. Peters T. “Total protein: direct Biuret method”. Clinical Chemistry 14 (1968): 1147-1159.
  20. Busher JT. “Clinical Methods: The History, Physical, and Laboratory Examinations”. Chapter 10: Serum Albumin and Globulin. 3rdWalker HK, Hall WD, Hurst JW (editors). Boston, Butterworths (1990).
  21. Moore JF and Sharer JD. “Methods for quantitative creatinine determination”. Current Protocols in Human Genetics 93.1 (2017): A.3O.1-A.3O.7.
  22. Patton CJ and Crouch S. “Spectrophotometric and kinetics investigation of the Berthelot reaction for the determination of ammonia”. Analytical Chemistry 49.3 (1977): 464-469.
  23. Stevens JF., et al. “Measurement of the enzymes lactate dehydrogenase and creatine kinase using reflectance spectroscopy and reagent strips”. Journal of Clinical Pathology 12 (1983): 1371-1376.
  24. Breckenridge C., et al. “A 30-day toxicity study of inhaled fenitrothion in the albino rat”. Toxicology and Applied Pharmacology.62.1 (1982): 32-43.
  25. Kalender Y., et al. “Effects of diazinon on pseudocholinesterase activity and haematological indices in rats: the protective role of vitamin E”. Environmental Toxicology and Pharmacology 22.1 (2006): 46-51.
  26. Mossa ATH., et al. “Physiological and histopathological changes in the liver of male rats exposed to paracetamol and diazinon”. Asian Pacific Journal of Tropical Biomedicine 2.3 ( 2012): S1683-S1690.
  27. Mansour SA and Mossa ATH. “Adverse effects of exposure to low doses of chlorpyrifos in lactating rats”. Toxicology and Industrial Health 27.3 (2011): 213-224.
  28. Stromborg K. Reproductive tests of diazinon on bobwhite quail. Paper presented at the Avian and mammalian wildlife toxicology: Second Conference (1981).
  29. Pakzad M., et al. “Sublethal exposures of diazinon alters glucose homostasis in Wistar rats: Biochemical and molecular evidences of oxidative stress in adipose tissues”. Pesticide Biochemistry and Physiology 105.1 (2013): 57-61.
  30. Al-Attar AM and Abu Zeid IM. “Effect of tea (Camellia sinensis) and olive (Olea europaea L.) leaves extracts on male mice exposed to diazinon”. BioMed Research International (2013): 461415.
  31. El-Shenawy NS., et al. “Amelioratory effect of vitamin E on organophosphorus insecticide diazinon-induced oxidative stress in mice liver”. Pesticide Biochemistry and Physiology 96.2 (2010): 101-107.
  32. Abdollahi M., et al. “Oxidative stress in aging”. Oxidative Medicine and Cellular Longevity. Hindawi, (2014).
  33. Inyang-Etoh P., et al. “Height as a surrogate for bodyweight for estimating the dosage of praziquantel and artesunate in the treatment of urinary schistosomiasis in school-age children”. Mary Slessor Journal of Medicine 10.1 (2010): 10-14.
  34. Shah PC., et al. “Phytase production by Aspergillus niger NCIM 563 for a novel application to degrade organophosphorus pesticides”. AMB Express 7.1 (2017): 1-11.
  35. Muthuviveganandavel V., et al. “Biochemical evaluation of low dose methyl 2-benzimidazole carbamate fungicide on male albino rats”. International Journal of Drug Delivery 2.4 (2010): 352-356.
  36. Zarn JA., et al. “Azole fungicides affect mammalian steroidogenesis by inhibiting sterol 14 alpha-demethylase and aromatase”. Environmental Health Perspectives 111.3 (2003): 255-261.
  37. Agbor GA., et al. “Hepatoprotective activity of Hibiscus cannabinus (Linn.) against carbon tetrachloride and paracetamol induced liver damage in rats”. Pakistan Journal of Biological Sciences 10 (2005): 1397-1401.
  38. Abdou HM and El Mazoudy RH. “Oxidative damage, hyperlipidemia and histological alterations of cardiac and skeletal muscles induced by different doses of diazinon in female rats”. Journal of Hazardous Materials 1-3 (2010): 273-278.
  39. Jafari-Dehkordi E and Seidkhani-Nahal A. “Lipid-lowering effect of artichoke on liver phosphatidate phosphohydrolase and plasma lipids in hyperlipidemic rats”. Journal of Medicinal Plants Research 5.19 (2011): 4918-4924.
  40. Shah MD and Iqbal M. “Diazinon-induced oxidative stress and renal dysfunction in rats”. Food and Chemical Toxicology 48.12 (2010): 3345-3353.
  41. Cayir K., et al. “Protective effect of L-carnitine against cisplatin-induced liver and kidney oxidant injury in rats”. Central European Journal of Medicine 4.2 (2009): 184-191.
  42. Zari TA and Al-Attar AM. “Therapeutic effects of olive leaves extract on rats treated with a sublethal concentration of carbendazim”. European Review for Medical and Pharmacological Sciences 15.4 (2011): 413-426.
  43. Karimi-Khouzani O., et al. “Anti-inflammatory and ameliorative effects of gallic acid on fluoxetine-induced oxidative stress and liver damage in rats”. Pharmacological Reports 69.4 (2017): 830-835.
  44. Ahmadi-Naji R., et al. “Evaluation of the effects of the hydroalcoholic extract of Terminalia chebula fruits on diazinon-induced liver toxicity and oxidative stress in rats”. Avicenna Journal of Phytomedicine 7.5 (2017): 454-466.
  45. Hassani S., et al. “Alteration of hepatocellular antioxidant gene expression pattern and biomarkers of oxidative damage in diazinon-induced acute toxicity in Wistar rat: a time-course mechanistic study”. EXCLI Journal 17 (2018): 57-71.
  46. Lari P., et al. “Evaluation of diazinon-induced hepatotoxicity and protective effects of crocin”. Toxicology and Industrial Health 31.4 (2015): 367-376.
  47. Al-Seeni MN., et al. “The hepatoprotective activity of olive oil and Nigella sativa oil against CCl 4 induced hepatotoxicity in male rats”. BMC Complementary and Alternative Medicine 16.1 (2016): 1-14.
  48. Kılıcoglu B., et al. “The ultrastructural research of liver in experimental obstructive jaundice and effect of honey”. The American Journal of Surgery 195.2 (2008): 249-256.
  49. Jalili S., et al. “Histopathological observations on protective effects of vitamin E on endosulfan induced cardiotoxicity in rats”. Pakistan Journal of Biological Sciences: PJBS 10.11 (2007): 1922-1925.

Citation

Citation: Mohsen Ali Khormi., et al. “Protective Effects of Manuka Honey Against Diazinon Induced Hepatorenal Toxicity in Adult Male Rats”.Acta Scientific Medical Sciences 6.6 (2022): 09-22.

Copyright

Copyright: © 2022 Mohsen Ali Khormi., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




Metrics

Acceptance rate30%
Acceptance to publication20-30 days
Impact Factor1.403

Indexed In





News and Events


  • Certification for Review
    Acta Scientific certifies the Editors/reviewers for their review done towards the assigned articles of the respective journals.
  • Submission Timeline for Upcoming Issue
    The last date for submission of articles for regular Issues is July 10, 2022.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Article of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of “Best Article of the Issue”.
  • Welcoming Article Submission
    Acta Scientific delightfully welcomes active researchers for submission of articles towards the upcoming issue of respective journals.
  • Contact US