Potential Nucleotides of CD38 are Causing the Aggregation of Platelets
Associate Professor, Basic Medical Sciences, Sulaiman Al Rajhi University, Saudi Arabia
*Corresponding Author: Mazhar Mushtaq, Associate Professor,
Basic Medical Sciences, Sulaiman Al Rajhi University, Saudi Arabia.
February 17, 2022; Published: March 15, 2022
Objective: To elucidate the platelets aggregating effects of CD38 produced nucleotides.
Introduction: The production of cADPR and NAADP has been demonstrated in various cells in different tissues. Their primary physiological response is to elicit an increase in intracellular calcium. Platelets, like other cells, are also calcium dependent on producing their physiological response, normal aggregation.
Materials and Methods: The platelets aggregating effect was studied in thermomax microplate reader. Calcium measurements were carried out using Fura 2-AM loaded platelets subjected to PTI in a magnetically stirred cuvette at 37 °C with proper excitation wavelengths. cADPR and NAADP assay were performed using a fluorescence reader. Thrombin and ADP were used as physiological agonist along with cADPR and NAADP. Values of the result were expressed as mean and were considered statistically significant if p < 0.05.
Result: By using Thrombin and ADP, we were able to delineate the pathway of cADPR and NAADP and their response to releasing calcium from the intracellular organelles. The use of specific inhibitors in the paths of these nucleotides enabled us to deduce that cADPR is upstream to that of NAADP.
Keywords: CD38; cADPR; NAADP; Platelets; Calcium Signal
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