Acta Scientific Medical Sciences (ASMS)(ISSN: 2582-0931)

Research Article Volume 5 Issue 12

Insect Lipase Activity as a Useful Indicator of Entomoremediation of Oil Contamination

Eman A Abdelfattah*

Entomology Department, Faculty of Science, Cairo University, Giza, Egypt

*Corresponding Author: Eman A Abdelfattah, Entomology Department, Faculty of Science, Cairo University, Giza, Egypt.

Received: August 16, 2021; Published: November 17, 2021


Anthropogenic releasing of oil into the environment is the main source of environmental pollution. Oil contamination is considered as one of hazardous materials to ecosystem components. The severity of damage may reach to all organisms through food chain and food web. So, the demand for creative and eco-friendly way is vital to decontaminate the deleterious effect of oil contamination. This study focuses on using lipase activity and oxidative stress parameters of black soldier fly larva (BSFL) as an indication of eco-friendly biodegradation tool of oil contamination. The results showed that, the insect lipase activity was significantly increased along time treatment (0-48h). Besides that, the lipid peroxidation concentration as a deleterious effect of oil contamination was decrease in the cuticle homogenates than the fat body. Also, the correlation between application time and lipase activity or lipid peroxides was strong to moderate positive correlation. These results emphasized the ability of using insect lipase activity and oxidative stress parameters as lipid peroxidation amount as a valuable indicator of entomoremediation of oil contamination.

Keywords: Hermetia illucens; Oil Contamination; Lipase; Lipid Peroxidation; Entomoremediation


  1. Cocârţă DM., et al. “Crude oil contaminated sites: evaluation by using risk assessment approach”. Sustainability8 (2017): 1365.‏
  2. Cecchi G., et al. “Port Sediments: Problem or Resource? A Review Concerning the Treatment and Decontamination of Port Sediments by Fungi and Bacteria”. Microorganisms6 (2021): 1279.‏
  3. Balba MT., et al. “Bioremediation of oil-contaminated soil: microbiological methods for feasibility assessment and field evaluation”. Journal of Microbiological Methods2 (1998): 155-164.‏
  4. Onwurah IN E., et al. “Crude oil spills in the environment, effects and some innovative clean-up biotechnologies” (2007).
  5. Bobic V. “Soil pollution with petroleum hydrocarbons-bioremediation: potentiality and practice”. Goriva I Maziva1 (2005): 20.‏
  6. Manisalidis I., et al. “Environmental and health impacts of air pollution: a review”. Frontiers in Public Health 8 (2020): 14.‏
  7. Environmental US. Protection Agency. Superfund Engineering Issue~ Treatment of Lead-Contaminated Soils. EPA/540/2-91/009. Office of Solid Waste and Emergency Response. Washington DC and Office of Research and Development, Risk Reduction Engineering Laboratory. Cincinnati, Ohio (1992).‏
  8. Saeed M., et al. “Development of a plant microbiome bioremediation system for crude oil contamination”. Journal of Environmental Chemical Engineering4 (2021): 105401.‏
  9. Jia J., et al. “The dynamic change of microbial communities in crude oil-contaminated soils from oil fields in China”. Soil and Sediment Contamination: An International Journal2 (2017): 171-183.‏
  10. Wu TY., et al. “Pollution control technologies for the treatment of palm oil mill effluent (POME) through end-of-pipe processes”. Journal of Environmental Management7 (2010): 1467-1490.‏
  11. Agamuthu P., et al. “Bioremediation of hydrocarbon contaminated soil using selected organic wastes”. Procedia Environmental Sciences 18 (2013): 694-702.‏
  12. Azeez NM and Sabbar AA. “Efficiency of duckweed (Lemna minor L.) in phytotreatment of wastewater pollutants from Basrah oil refinery”. Journal of Applied Phytotechnology in Environmental Sanitation4 (2012): 163-172.‏
  13. Wang ZL., et al. “Catalases play differentiated roles in the adaptation of a fungal entomopathogen to environmental stresses”. Environmental Microbiology2 (2013): 409-418.‏
  14. Naushad M. “Surfactant assisted nano-composite cation exchanger: development, characterization and applications for the removal of toxic Pb2+ from aqueous medium”. Chemical Engineering Journal 235 (2014): 100-108.‏
  15. Hararah MA., et al. “Zinc adsorption–desorption isotherms: possible effects on the calcareous vertisol soils from Jordan”. Environmental Earth Sciences7 (2012): 2079-2085.‏
  16. Naushad M., et al. “Ion-exchange kinetic studies for Cd (II): Co (II): Cu (II): and Pb (II) metal ions over a composite cation exchanger”. Desalination and Water Treatment10 (2015): 2883-2890.‏
  17. Sharma G and Naushad M. “Adsorptive removal of noxious cadmium ions from aqueous medium using activated carbon/zirconium oxide composite: isotherm and kinetic modelling”. Journal of Molecular Liquids 310 (2020): 113025.‏
  18. Bacosa HP and Inoue C. “Polycyclic aromatic hydrocarbons (PAHs) biodegradation potential and diversity of microbial consortia enriched from tsunami sediments in Miyagi, Japan”. Journal of Hazardous Materials 283 (2015): 689-697.‏
  19. Tirandaz H., et al. “The succession of Dominant Culturable Hydrocarbon-Utilizing Bacteria During Bioremediation of Oil-Based Drilling Waste”. Iranian Journal of Chemistry and Chemical Engineering5 (2019): 267-277.‏
  20. Fatima K., et al. “Successful phytoremediation of crude-oil contaminated soil at an oil exploration and production company by plants-bacterial synergism”. International Journal of Phytoremediation7 (2018): 675-681.‏
  21. Smarzewska S and Guziejewski D. Soil remediation technologies. In “Handbook of Advanced Approaches Towards Pollution Prevention and Control”. Elsevier (2021): 193-219
  22. Abdelfattah EA. “Biomolecule’s oxidation and antioxidant enzymes response as a result of injection of oxidative stressor into 5th instar of Schistocerca gregaria (Orthoptera, Acrididae)”. Entomology, Ornithology and Herpetology 5181 (2016): 2161-2983.‏
  23. Renault D., et al. “Assessment of oxidative stress and activities of antioxidant enzymes depicts the negative systemic effect of iron-containing fertilizers and plant phenolic compounds in the desert locust”. Environmental Science and Pollution Research21 (2016): 21989-22000.‏
  24. Abdelfattah EA., et al. “Biomonitoring of genotoxicity of industrial fertilizer pollutants in Aiolopus thalassinus (Orthoptera: Acrididae) using alkaline comet assay”. Chemosphere 182 (2017): 762-770.‏
  25. Yousef HA., et al. “Evaluation of oxidative stress biomarkers in Aiolopus thalassinus (Orthoptera: Acrididae) collected from areas polluted by the fertilizer industry”. Ecotoxicology 3 (2017): 340-350.‏
  26. Yousef HA., et al. “Antioxidant enzyme activity in responses to environmentally induced oxidative stress in the 5th instar nymphs of Aiolopus thalassinus (Orthoptera: Acrididae)”. Environmental Science and Pollution Research4 (2019): 3823-3833.‏
  27. NASSAR MI., et al. “BEE venom drug potentiality on the macromolecules damage of the larval gut of hermetia illucens (l.): (diptera: stratiomyidae)”. Journal of the Egyptian Society of Parasitology3 (2020): 488-493.
  28. Abdelfattah EA., et al. “Stage-, sex-and tissue-related changes in H2O2, glutathione concentration, and glutathione-dependent enzymes activity in Aiolopus thalassinus (Orthoptera: Acrididae) from heavy metal polluted areas”. Ecotoxicology3 (2021): 478-491.‏
  29. Cammack JA and Tomberlin JK. “The impact of diet protein and carbohydrate on select life-history traits of the Black Soldier Fly Hermetia illucens (L.) (Diptera: Stratiomyidae)”. Insects 2 (2017): 56.
  30. Imlay JA. “Pathways of oxidative damage”. Annual Review of Microbiology 57 (2003): 395-418.
  31. Stadtman ER and Levine RL. “Free radical-mediated oxidation of free amino acids and amino acid residues in proteins”. Amino Acids 25 (2003): 207-218.
  32. Rosa CE., et al. “Antioxidiant responses of Laeonereis acute (Polychaeta) after exposure of hydrogen peroxide”. Brazilian Journal of Medical and Biological Research 41 (2008): 117-121.
  33. Kodrik D., et al. “Hormonal regulation of response to oxidative stress in insects- An update”. International Journal of Molecular Sciences 16 (2015): 25788-25816.
  34. Abd-Elhakeem MA., et al. “New colorimetric method for lipases activity assay in microbial media”. American Journal of Analytical Chemistry (2013‏).
  35. Hermes-Lima M., et al. “Quantification of lipid peroxidation in tissue extracts based on Fe (III) xylenol orange complex formation”. Free Radical Biology and Medicine3 (1995): 271-280.
  36. Bradford MM. “A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding”. Analytical Biochemistry1 (1976): 248-254.
  37. Ewuim SC. “Entomoremediation-a novel in-situ bioremediation approach”. Animal Research International1 (2013): 1681-1684.‏
  38. Yang J., et al. “Evidence of polyethylene biodegradation by bacterial strains from the guts of plastic-eating waxworms”. Environmental Science and Technology23 (2014): 13776-13784.‏
  39. Yang Y., et al. “Biodegradation and mineralization of polystyrene by plastic-eating mealworms: Part 1. Chemical and physical characterization and isotopic tests”. Environmental Science and Technology 20 (2015): 12080-12086.‏
  40. Berasategui A., et al. “Potential applications of insect symbionts in biotechnology”. Applied Microbiology and Biotechnology4 (2016): 1567-1577.‏
  41. Bulak P., et al. “Hermetia illucens as a new and promising species for use in entomoremediation”. Science of the Total Environment 633 (2018): 912-919.‏
  42. Rodriguez-Campos J., et al. “Bioremediation of soil contaminated by hydrocarbons with the combination of three technologies: bioaugmentation, phytoremediation, and vermiremediation”. Journal of Soils and Sediments4 (2019): 1981-1994.‏
  43. de Oliveira VP and Cruz-Landim C. “Morphology and function of insect fat body cells: a review”. Biociências 11 (2003): 195-205.‏
  44. Kannan K and Jain SK. “Oxidative stress and apoptosis”. Pathophysiology 7 (2000): 153-163.
  45. Yao HP., et al. “Identification of the proteome of the midgut of silkworm, Bombyx mori L., by multidimensional liquid chromatography (MDLC) LTQ-Orbitrap MS”. Bioscience Reports 29 (2009): 363-373.
  46. Abdelfattah EA and Renault D. “Effect of different doses of the catecholamine epinephrine on antioxidant responses of larvae of the flesh fly Sarcophaga dux”. Environmental Pollution Research Science (2021): 1-8.
  47. Abdelfattah EA. “Effect of different concentration and application time of vitamin B12 on antioxidant response of Physiophora alceae”. African Journal of Biological Sciences (2021): 189-203.
  48. Abdelfattah EA and Lim JW. “Biotechnology application of organic waste management using black soldier fly, Hermetia illucens”. African Journal of Biological Sciences1 (2021): 171-187.‏


Citation: Eman A Abdelfattah. “Insect Lipase Activity as a Useful Indicator of Entomoremediation of Oil Contamination”.Acta Scientific Medical Sciences 5.12 (2021): 81-88.


Copyright: © 2021 Eman A Abdelfattah. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


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