Acta Scientific Medical Sciences (ASMS)(ISSN: 2582-0931)

Research Article Volume 5 Issue 6

B-SHOT a New Technique in Genital Area Rejuvenation; Co-Injection of Stromal Vascular Fraction and Adipose-Derived Mesenchymal Stem Cell

Bülent Cihantimur*, Özgür Ağlamiş and Yavuz Özsular

Department of Plastic Surgery, Estetik International Quasar Clinic, Turkey

*Corresponding Author: Bülent Cihantimur, Department of Plastic Surgery, Estetik International Quasar Clinic, Turkey.

Received: January 20, 2021; Published: May 29, 2021

Abstract

Purpose: We named the co-injection of stromal vascular fraction (SVF) and adipose-derived mesenchymal stem cell, which are cells with the highest regenerative capacity of the body derived from adipose tissue, as B-SHOT rejuvenation and investigated whether this new technique is effective in genital area rejuvenation.

Material and Method: 40 female participants participated in the present study. 40 cc lipoaspiration material was reached from the abdominal region. By using fat micronizing device, lipoaspirate was subjected to non-enzymatic mechanical filtration up to 400 microns and then centrifuged. The obtained Stromal Vascular Fraction (FAT JUICE) and adipose derived mesenchymal stem cells were injected with our B-SHOT technique to the clitoris, around the openings of skene glands, vaginal mucosa, and G spot area. All participants were evaluated with VHI and FSFI before and after the application in the 3rd, 6th, 9th and 12th months.

Results: Elasticity, vaginal pH and humidity scores from VHI parameters significantly increased in the 3rd and 6th months after treatment (p < 0.001). In addition, significant increase in the score of the new parameter continued in the 9th month, as well (p < 0.05). Scores of orgasm, satisfaction and pain among FSFI parameters significantly increased in the 3rd and 6th months after treatment.

Conclusion: Our B-SHOT technique may be a new practical rejuvenation protocol for genital rejuvenation, which is a low cost, safe, effective technique, prepared within a short time without the need for additional staff or laboratory and benefits from the regenerative effect of adipose derived mesenchymal stem cells in SVF.

Keywords: B-SHOT; Genital Rejuvenation; Adipose Derived Mesenchymal Stem Cell; Stromal Vascular Fraction

References

  1. Rigotti G., et al. “Adipose-Derived Mesenchymal Stem Cells: Past, Present, and Future”. Aesthetic Plastic Surgery 33 (2009): 271-273.
  2. Clauser L., et al. “Autologous Fat Transfer for Facial Augmentation and Regeneration”. Atlas of the Oral and Maxillofacial Surgery Clinics of North America 26 (2018): 25-32.
  3. Zuk PA., et al. “Human Adipose Tissue Is a Source of Multipotent Stem Cells”. Molecular Biology of the Cell 13 (2002): 4279-4295.
  4. Hass R., et al. “Different populations and sources of human mesenchymal stem cells (MSC): A comparison of adult and neonatal tissue-derived MSC”. Cell Communication and Signaling 9 (2011): 12.
  5. Rodbell M. “Metabolism of isolated fat cells”. Journal of Biological Chemistry 239 (1964): 375-380.
  6. Mizuno H., et al. “Concise Review: Adipose-Derived Stem Cells as a Novel Tool for Future Regenerative Medicine”. Stem Cells 30 (2012): 804-810.
  7. Horwitz EM., et al. “Transplantability and therapeutic effects of bone marrow-derived mesenchymal cells in children with osteogenesis imperfecta”. Nature Medicine 5 (1999): 309-313.
  8. Schäffler A and Büchler C. “Concise Review: Adipose Tissue-Derived Stromal Cells-Basic and Clinical Implications for Novel Cell-Based Therapies”. Stem Cells 25 (2007): 818-827.
  9. Kasir R., et al. “Regenerative Engineering of Cartilage Using Adipose-Derived Stem Cells”. Regenerative Engineering and Translational Medicine 1 (2015): 42-49.
  10. Shah FS., et al. “A non-enzymatic method for isolating human adipose tissue-derived stromal stem cells”. Cytotherapy 15 (2013): 979-985.
  11. Tonnard P., et al. “Nanofat Grafting”. Plastic and Reconstructive Surgery 132 (2013): 1017-1026.
  12. Bachmann G. “Urogenital ageing: an old problem newly recognized”. Maturitas 22 (1995): S1-S5.
  13. Sand M., et al. “The female sexual function index (FSFI): a potential “gold standard” measure for assessing therapeutically-induced change in female sexual function”. Fertility and Sterility 92 (2009): S129.
  14. Wiegel M., et al. “The Female Sexual Function Index (FSFI): Cross-Validation and Development of Clinical Cutoff Scores”. Journal of Sex and Marital Therapy 31 (2005): 1-20.
  15. Bora P and Majumdar AS. “Adipose tissue-derived stromal vascular fraction in regenerative medicine: a brief review on biology and translation”. Stem Cell Research and Therapy 8 (20179): 145.
  16. Aronowitz JA., et al. “Mechanical versus enzymatic isolation of stromal vascular fraction cells from adipose tissue”. Springer Plus 4 (2015): 713.
  17. Senesi L., et al. “Mechanical and Enzymatic Procedures to Isolate the Stromal Vascular Fraction From Adipose Tissue: Preliminary Results”. Frontiers in Cell and Developmental Biology 7 (2019): 88.
  18. Condé-Green A., et al. “Shift toward Mechanical Isolation of Adipose-derived Stromal Vascular Fraction”. Plastic and Reconstructive Surgery 4 (2016): e1017.
  19. Lin K., et al. “Characterization of adipose tissue-derived cells isolated with the Celution™ system”. Cytotherapy 10 (2008): 417-426.
  20. Amirkhani MA., et al. “Rejuvenation of facial skin and improvement in the dermal architecture by transplantation of autologous stromal vascular fraction: a clinical study”. Bioimpacts 6 (2016): 149-154.
  21. Cardoso AL., et al. “Adipose tissue stromal vascular fraction in the treatment of full thickness burns in rats”. Acta Cirúrgica Brasileira 31 (2016): 578-585.
  22. Rigotti G., et al. “Expanded Stem Cells, Stromal-Vascular Fraction, and Platelet-Rich Plasma Enriched Fat: Comparing Results of Different Facial Rejuvenation Approaches in a Clinical Trial”. Aesthetic Surgery Journal 36 (2016): 261-270.
  23. Klar AS., et al. “Characterization of vasculogenic potential of human adipose-derived endothelial cells in a three-dimensional vascularized skin substitute”. Pediatric Surgery International 32 (2015): 17-27.
  24. Aggarwal S. “Human mesenchymal stem cells modulate allogeneic immune cell responses”. Blood 105 (2005): 1815-1822.
  25. Cheng C., et al. “Cell-Assisted Skin Grafting”. Plastic and Reconstructive Surgery 137 (2016): 58-66.
  26. Gentile P., et al. “Comparing different nanofat procedures on scars: role of the stromal vascular fraction and its clinical implications”. Regenerative Medicine 12 (2017): 939-9952.
  27. Tamburino S., et al. “The Role of Nanofat Grafting in Vulvar Lichen Sclerosus: A Preliminary Report”. Archives of Plastic Surgery 43 (2016): 93-95.
  28. Casabona F., et al. “New Surgical Approach to Lichen Sclerosus of the Vulva: The Role of Adipose-Derived Mesenchymal Cells and Platelet-Rich Plasma in Tissue Regeneration”. Plastic and Reconstructive Surgery 126 (2010): 210-211.
  29. Menkes S., et al. “Microfat and Nanofat Grafting in Genital Rejuvenation”. Aesthetic Surgery Journal (2020).
  30. Vanaman M., et al. “Emerging Trends in Nonsurgical Female Genital Rejuvenation”. Dermatology Surgery 42 (2016): 1019-1029.
  31. Park HJ., et al. “Hyaluronic acid pulmonary embolism: a critical consequence of an illegal cosmetic vaginal procedure”. Thorax 65 (2010): 360-361.
  32. Duan Y., et al. “Polyacrylamide hydrogel pulmonary embolism—A fatal consequence of an illegal cosmetic vaginal tightening procedure: A case report”. Forensic Science International 238 (2014): 6-10.

Citation

Citation: Bülent Cihantimur., et al. “B-SHOT a New Technique in Genital Area Rejuvenation; Co-Injection of Stromal Vascular Fraction and Adipose-Derived Mesenchymal Stem Cell”.Acta Scientific Medical Sciences 5.6 (2021): 158-166.

Copyright

Copyright: © 2021 Bülent Cihantimur., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

div>



Metrics

Acceptance rate30%
Acceptance to publication20-30 days
Impact Factor1.403

Indexed In





Contact US