Acta Scientific Medical Sciences (ASMS)(ISSN: 2582-0931)

Research Article Volume 5 Issue 6

Storage Dependent Cellular Changes in Blood Smears Prepared from EDTA Added Venous Blood Samples

Rintu P Samu, Shuhaib S, Deepa Revi* and Rahul Raj A R

Medical Trust Institute of Medical Sciences, Medical Trust Hospital, Ernakulam, Kerala, India India

*Corresponding Author: Deepa Revi, Assistant Professor, Medical Trust Institute of Medical Science and Technology, Medical Trust Hospital, Ernakulam, Kerala, India. E-mail:

Received: April 24, 2021; Published: May 14, 2021



Ethylene diamine tetra acetate (EDTA) added blood is commonly used to perform various analysis like the complete blood count in a hematology laboratory. Prolonged storage in EDTA can cause morphological changes in the cellular components of the blood which has some potential to tamper with the diagnosis and interpretation of results especially during peripheral blood smear reporting and differential counting. This study aims to assess the morphology related variations in WBC and RBC in peripheral smears prepared from EDTA added blood stored at room temperature at different time periods. Peripheral smears were prepared from 20 healthy volunteers after 0 hrs, 2 hrs, 5 hours, 10 hours and 24 hours of storage in EDTA added blood collection containers. Parameters affecting cellular morphology such as crenation, acanthocytes, bite cells, fragmentation and central pallor (for RBCs) and nuclear degeneration, number of lobes in nucleus and vacuolation in cytoplasm (for WBCs) were quantified microscopically in 10 fields/smear after Leishman staining. Students t-test was performed to determine if there was significant morphological variation at different time points and p value less than 0.05 was considered significant. There was significant increase in the number of RBCs with crenation, acanthocytes, bite cells in smears prepared after 2 hours while WBCs showed significant nuclear degeneration and cytoplasmic changes in smears prepared after 1 hour of storage. Maximum cellular distortion was seen in smears prepared after 24 hours of storage at room temperature. Morphological interpretations of RBCS should preferentially be done within 2 hours while for differential count should preferentially be done within 1 hour to minimize errors and misdiagnosis, when blood samples are stored at room temperature.

Keywords: Complete Blood Cell (CBC); Ethylene Diamine Tetra Acetate (EDTA); Crenation; Bite Cells; Acanthocytes; Nuclear Degeneration; Cytoplasmic Vacuolation



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Citation: Deepa Revi., et al. “Storage Dependent Cellular Changes in Blood Smears Prepared from EDTA Added Venous Blood Samples”.Acta Scientific Medical Sciences 5.6 (2021): 42-46.


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