Acta Scientific Medical Sciences (ASMS)(ISSN: 2582-0931)

Research Article Volume 4 Issue 12

Zingiber officinale (Ginger) Extract has No Effect on Kiss1 Gene Expression in the Testis and Blood but May Cause Inflammation-induced Morphological Sperm Disruptions in Wistar Rats

Izuchukwu Azuka Okafor1,2,3*, Uchenna Somtochukwu Nnamah4, Johnson Okwudili Nweke5, Jude Amechi Nnaka1, Selasie Ahiatrogah2,3 and Uche Valentine Okeke6

1Department of Anatomy, Faculty of Basic Medical Sciences, College of Health Sciences, Nnamdi Azikiwe University, Nnewi, Nigeria
2Department of Obstetrics and Gynaecology, College of Medicine, University of Ibadan, Ibadan, Nigeria
3Pan African University of Life and Earth Science Institute (Including Health and Agriculture), PAULESI, University of Ibadan, Ibadan, Nigeria
4Hematology Department, Babcock University Teaching Hospital, Ilisan Remo, Ogun State, Nigeria
5Diagnostic Laboratory Unit, Medical Centre, Michael Okpara University of Agriculture, Umudike, Abia State, Nigeria
6Morbid Anatomy Department, Alex Ekwueme Federal University Teaching Hospital Abakaliki, Ebonyi State, Nigeria

*Corresponding Author: Izuchukwu Azuka Okafor, Department of Anatomy, Faculty of Basic Medical Sciences, College of Health Sciences, Nnamdi Azikiwe University, Nnewi, Nigeria.

Received: September 23, 2020; Published: November 18, 2020



Background: Zingiber officinale is used extensively both as a culinary spice and herbal medicine for the treatment of different ailments.

Aim: This study investigated the role of ginger in the Kiss1 gene expression in the testis of Wistar rats.

Methods: Fifteen (15) rats with an average weight of 104g were randomly divided into three (3) groups (A-C). Group A was given no treatment and served as the normal control group. Groups B and C received oral administration of 200 mg/kg and 400 mg/kg of methanolic extract of Zingiber officinale (MEZO) respectively. The extract was administered once a day for 21 days.

Results: There was no significant difference in the testicular oxidative status, LH, FSH, TT, sperm motility, sperm count, and sperm morphology across all experimental groups (p < 0.05) except for group C where a significant decrease in sperm morphology was observed compared to the control (p = 0.007). Some vascular congestions with visible aggregates of inflammatory red cells were also observed in group C testes. The relative intensity of Kiss1 gene expression observed in the testis is significantly higher than that in the blood at 200 mg/kg of MEZO (CI: -1.39 - (-0.05), p = 0.045) with a significant negative correlation between the sperm count and the relative intensity of KISS1 gene expression in the blood (r = -.882, p = 0.20).

Conclusion: MEZO does not affect the Kiss1 gene expression levels in the testis and blood of Wistar rats but shows the potential of causing sperm morphological disruptions due to testicular inflammatory response at a high dose.

Keywords: Zingiber officinale; Sperm Count; Sperm Morphology; Sperm Motility; Kiss1; Kisspeptin; Ginger; Gene Expression; Hormones; Testis; Histology; Oxidative Markers



  1. White B. “Ginger: An overview”. American Family Physician 11 (2007): 1689-1691.
  2. Ali BH., et al. “Some phytochemical, pharmacological, and toxicological properties of ginger: a review of recent research”. Food and Chemical Toxicology2 (2008): 409-420.
  3. Jolad SD., et al. “Fresh organically grown ginger (Zingiber officinale): composition and effects on LPS-induced PGE2 production”. Phytochemistry 65 (2004): 1937-1954.
  4. Yeh H yu., et al. “Bioactive components analysis of two various gingers (Zingiber officinale Roscoe) and antioxidant effect of ginger extracts”. LWT - Food Science and Technology1 (2014): 329-334.
  5. Khan AM., et al. “Zingiber officinale ameliorates allergic asthma via suppression of Th2-mediated immune response”. Pharmaceutical Biology3 (2015).
  6. Lantz R C., et al. “The effect of extracts from ginger rhizome on inflammatory mediator production”. Phytomedicine 14 (2007): 123-128.
  7. Bak M J., et al. “6-shogaol-rich extract from ginger up-regulates the antioxidant defense systems in cells and mice”. Molecules7 (2012) 8037-8055.
  8. Hosseinzadeh A., et al. “Protective Effect of Ginger (Zingiber officinale Roscoe) Extract against Oxidative Stress and Mitochondrial Apoptosis Induced by Interleukin-1β in Cultured Chondrocytes”. Cells Tissues Organs 204 (2017): 241-250.
  9. Islam K., et al. “Antimicrobal Activity of Ginger (Zingiber Officinale) Extracts Against Food-Borne Pathogenic Bacteria”. International Journal of Science, Environment and Technology (2014).
  10. Rhode J., et al. “Ginger inhibits cell growth and modulates angiogenic factors in ovarian cancer cells”. BMC Complementary and Alternative Medicine (2007).
  11. Oda S S and Waheeb R S. “Ginger attenuated DI (N-butyl) phthalate-induced reproductive toxicity in pubertal male rabbits”. World Rabbit Science (2018).
  12. Bordbar H., et al. “Stereological study of the effect of ginger’s alcoholic extract on the testis in busulphan-induced infertility in rats”. International Journal of Reproductive BioMedicine. 11.6 (2013): 467-472.
  13. Silveira LG., et al. “Mutations of the KISS1 gene in disorders of puberty”. Journal of Clinical Endocrinology and Metabolism 5 (2010): 2276-2280.
  14. Shirasaki F., et al. “Loss of expression of the metastasis suppressor gene Kiss1 during melanoma progression and its association with LOH of chromosome 6q16.3-q23”. Cancer Research20 (2001): 7422-7425.
  15. Wolfe A and Hussain MA. “Emerging roles for kisspeptin in metabolism in mammals”. Frontiers in Endocrinology 9 (2018): 184.
  16. Rhie YJ. “Kisspeptin/ G protein-coupled receptor-54 system as an essential gatekeeper of pubertal development”. American Pediatrics Endocrinology Metabolism 18 (2013): 55-59.
  17. Tena-Sempere M. “Roles of Kisspeptins in the control of hypothalamic-gonadotropic function, Focus on sexual differentiation and puberty onset”. Endocrinology Development 17 (2010): 52-62.
  18. Hu K L., et al. “Kisspeptin/Kisspeptin Receptor System in the Ovary”. Frontiers in Endocrinology 8 (2018): 365.
  19. Tariq A., et al. “KISS1 and KISS1 receptor expression in the rhesus monkey testis: A possible local regulator of testicular function”. Central European Journal of Biology 8 (2013): 968-974.
  20. Dudek M., et al. “Effects of high-fat diet-induced obesity and diabetes on Kiss1 and GPR54 expression in the hypothalamic-pituitary-gonadal (HPG) axis and peripheral organs (fat, pancreas, and liver) in male rats”. Neuropeptides 56 (2016): 41-49.
  21. Leon S., et al. “Direct actions of kisspeptins on GnRH neurons permit attainment of fertility but are sufficiency to fully preserve gonadotropic axis activity”. Scientific Reproduction 6 (2016): 1-13.
  22. Ahmadifar E., et al. “Can dietary ginger (Zingiber officinale)alter biochemical and immunological parameters and gene expression related to growth, immunity and antioxidant system in zebrafish (Daniorerio)?”. Aquaculture (2019).
  23. Elkady A I., et al. “Differential control of growth, apoptotic activity, and gene expression in human breast cancer cells by extracts derived from medicinal herbs Zingiber officinale”. Journal of Biomedicine and Biotechnology (2012).
  24. Okafor I A and Ezejindu D N. “Phytochemical studies on Portulaca oleracea (purslane) plant”. Global Journal of Biology, Agriculture and Health Sciences1 (2014): 132-136.
  25. Okafor IA and Gbotolorun SC. “Resveratrol prevents cisplatin-induced lipid peroxidation in the non-gravid uterus of Sprague-Dawley rats”. Middle East Fertility Society Journal 23 (2018): 205-210.
  26. Ravindran P N and Babu K N. “Ginger: The genus Zingiber”. In Ginger: The Genus Zingiber (2016).
  27. Sellers R S., et al. “Society of Toxicologic Pathology position paper: organ weight recommendations for toxicology studies”. In Toxicologic pathology (2007).
  28. Chianese R., et al. “Kisspeptins, Estrogens and Male Fertility”. Current Medicinal Chemistry (2016).
  29. Aytürk N., et al. “The effect of kisspeptin on spermatogenesis and apoptosis in rats”. Turkish Journal of Medical Sciences1 (2017): 334-342.
  30. Afzali A and Ghalehkandi JG. “Effect of ginger, Zingiber officinale on sex hormones and certain biochemical parameters of male Wistar rats”. 11.1 (2018): 181-186.
  31. Ataman JE and Ojukwu F. “Protective Effects of Aqueous Extract of Ginger on Castor-oil - Induced Testicular Damage in Wistar Rats”. 14.4 (2014): 199-205.


Citation: Izuchukwu Azuka Okafor., et al. “Zingiber officinale (Ginger) Extract has No Effect on Kiss1 Gene Expression in the Testis and Blood but May Cause Inflammation-induced Morphological Sperm Disruptions in Wistar Rats". Acta Scientific Medical Sciences 4.12 (2020): 17-25.


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