Acta Scientific Medical Sciences (ASMS)(ISSN: 2582-0931)

Research Article Volume 4 Issue 11

The Role of Zingiber officinale (Ginger) Extract in the Kiss1 Gene Expression in the Ovary and Blood of Wistar Rats

Izuchukwu Azuka Okafor1,2,3*, Uchenna Somtochukwu Nnamah4, Johnson Okwudili Nweke5, Jude Amechi Nnaka1, Selasie Ahiatrogah2,3, Uche Valentine Okeke6 and Chukwuemerie Christian Okoro1

1Department of Anatomy, Faculty of Basic Medical Sciences, College of Health Sciences, Nnamdi Azikiwe University, Nnewi Campus, Nnewi, Nigeria
2Department of Obstetrics and Gynaecology, College of Medicine, University of Ibadan, Ibadan, Nigeria
3Pan African University of Life and Earth Science Institute (Including Health and Agriculture), PAULESI, University of Ibadan, Ibadan, Nigeria
4Hematology Department, Babcock University Teaching Hospital, Ilisan Remo, Ogun State, Nigeria
5Diagnostic Laboratory Unit, Medical Centre, Michael Okpara University of Agriculture, Umudike, Abia State, Nigeria
6Morbid Anatomy Department, Alex Ekwueme Federal University Teaching Hospital Abakaliki, Ebonyi State, Nigeria

*Corresponding Author: Izuchukwu Azuka Okafor, Department of Anatomy, Faculty of Basic Medical Sciences, College of Health Sciences, Nnamdi Azikiwe University, Nnewi Campus, Nigeria.

Received: September 23, 2020; Published: October 22, 2020



Background: Ginger (Zingiber officinale) is one of the most commonly consumed dietary condiments and herbal supplements in the world.

Aim: This study investigated the effect of methanolic extract of Zingiber officinale (MEZO) on the expression level of the Kiss1 gene in the blood and ovary of Wistar rats.

Methods: Fifteen (15) Wistar rats with an average weight of 147g were randomly divided into three (3) groups (A-C). Group A was given no treatment and served as the normal control group. Groups B and C received only oral administration of 200 mg/kg and 400 mg/kg of MEZO respectively. MEZO was administered once a day for 21 days.

Results: Group C showed a significantly reduced relative intensity of Kiss1 gene expression in the blood compared to the control (p = 0.006). There is also a significant increase between the expression intensity observed in the blood compared to the ovary in each experimental group except in group C (group A = CI: 0.55 - 0.94, p = 0.013; group B = CI: 0.69 - 0.86, p = 0.001; group C= CI: -0.09 - 0.73, p = 0.076). However, there is no significant correlation (between the Kiss1 gene relative expression intensity and reproductive hormones. There is no significant difference in the hormonal levels, oxidative stress, and relative ovarian weights across the experimental groups (p > 0.05).

Conclusion: High dose of MEZO showed the potentials of causing the downregulation of Kiss1 gene expression in the blood without any corresponding change in the reproductive hormonal levels.

Keywords: Zingiber officinale; Kiss1; Ovary; Blood; Oxidative Stress; Histology; Hormones; Gene Expression; Ginger; Kisspeptin



  1. Vasala PA. “Ginger”. In Handbook of Herbs and Spices: Second Edition (2012).
  2. Prasad S and Tyagi AK. “Ginger and its constituents: Role in prevention and treatment of gastrointestinal cancer”. Gastroenterology Research and Practice (2015).
  3. Khan AM., et al. “Zingiber officinale ameliorates allergic asthma via suppression of Th2-mediated immune response”. Pharmaceutical Biology (2015).
  4. Bak MJ., et al. “6-shogaol-rich extract from ginger up-regulates the antioxidant defense systems in cells and mice”. Molecules (2012).
  5. Yeh H yu., et al. “Bioactive components analysis of two various gingers (Zingiber officinale Roscoe) and antioxidant effect of ginger extracts. LWT - Food Science and Technology (2014).
  6. Chang JS., et al. “Fresh ginger (Zingiber officinale) has anti-viral activity against human respiratory syncytial virus in human respiratory tract cell lines”. Journal of Ethnopharmacology (2013).
  7. Bordbar H., et al. “Stereological study of the effect of ginger’s alcoholic extract on the testis in busulphan-induced infertility in rats”. International Journal of Reproductive BioMedicine 6(2013): 467-472.
  8. Shirasaki F., et al. “Loss of expression of the metastasis suppressor gene Kiss1 during melanoma progression and its association with LOH of chromosome 6q16.3-q23”. Cancer Research 20(2001):7422-7425.
  9. Yeo SH., et al. “Visualisation of Kiss1 Neurone Distribution Using a Kiss1-CRE Transgenic Mouse. Journal of Neuroendocrinology (2016).
  10. Ruohonen ST., et al. “Role of kisspeptins in the control of the hypothalamic-pituitary-ovarian axis: old dogmas and new challenges”. Fertility and Sterility (2020).
  11. R B D W and S I. “Dihydrotestosterone increases Kiss1 expression in adipose and pituitary tissue, but not in hypothalamus, in a rat model of polycystic ovary syndrome (PCOS)”. Endocrine Abstracts (2009).
  12. Castellano J M., et al. “Expression of Kiss1 in rat ovary: Putative local regulator of ovulation? Endocrinology10(2006): 4852-4862.
  13. Han Y., et al. “Local expressions and function of Kiss1/GPR54 in goats’ testes”. Gene (2020).
  14. Aloqaily B., et al. “SUN-261 Deletion of Hepatic Kisspeptin Results in Abnormal Glucose Metabolism in Female Mice”. Journal of the Endocrine Society (2020).
  15. Kim J., et al. “Regulation of Kiss1 expression by sex steroids in the amygdala of the rat and mouse”. Endocrinology (2011).
  16. Schäfer-Somi S., et al. “Kisspeptin-10 and the G protein-coupled receptor 54 are differentially expressed in the canine pregnant uterus and trophoblast cells”. Reproduction in Domestic Animals (2017).
  17. Tena-Sempere M. “Kisspeptin/GPR54 system as potential target for endocrine disruption of reproductive development and function”. International Journal of Andrology (2010).
  18. Silveira LG., et al. “Mutations of the KISS1 gene in disorders of puberty”. Journal of Clinical Endocrinology and Metabolism (2010).
  19. Gorkem U., et al. “Is there a role for kisspeptin in pathogenesis of polycystic ovary syndrome?” Gynecological Endocrinology (2018).
  20. Tang R., et al. “Kisspeptin and polycystic ovary syndrome”. Frontiers in Endocrinology (2019).
  21. Fan J., et al. “Effect of soyabean isoflavones exposure on onset of puberty, serum hormone concentration and gene expression in hypothalamus, pituitary gland and ovary of female bama miniature pigs. Asian-Australasian Journal of Animal Sciences (2015).
  22. Okafor IA., et al. “A review on Portulaca oleracea (Purslane) plant Its nature and biomedical benefits”. International Journal of Biomedical Research2(2014): 75-80.
  23. Okafor IA and Gbotolorun SC. “Resveratrol prevents cisplatin-induced lipid peroxidation in the non-gravid uterus of Sprague-Dawley rats”. Middle East Fertility Society Journal (2018).
  24. Abdulrazaq NB., et al. “Beneficial effects of ginger (Zingiber officinale) on carbohydrate metabolism in streptozotocin-induced diabetic rats”. British Journal of Nutrition (2012).
  25. Peng J., et al. “Kisspeptin stimulates progesterone secretion via the Erk1/2 mitogen-activated protein kinase signaling pathway in rat luteal cells”. Fertility and Sterility (2013).
  26. Afzali A and Ghalehkandi JG. “Effect of ginger, Zingiber officinale on sex hormones and certain biochemical parameters of male Wistar rats”. 11.1(2018): 181-186.
  27. Ahmadifar E., et al. “Can dietary ginger (Zingiber officinale)alter biochemical and immunological parameters and gene expression related to growth, immunity and antioxidant system in zebrafish (Daniorerio)?” Aquaculture (2019).
  28. Hosseinzadeh A., et al. “Protective Effect of Ginger (Zingiber officinale Roscoe) Extract against Oxidative Stress and Mitochondrial Apoptosis Induced by Interleukin-1β in Cultured Chondrocytes”. Cells Tissues Organs (2017).
  29. Pournaderi PS., et al. “The effects of 6-Gingerol on reproductive improvement, liver functioning and Cyclooxygenase-2 gene expression in estradiol valerate - Induced polycystic ovary syndrome in Wistar rats”. Biochemical and Biophysical Research Communications (2017).


Citation: Izuchukwu Azuka Okafor., et al. “The Role of Zingiber officinale (Ginger) Extract in the Kiss1 Gene Expression in the Ovary and Blood of Wistar Rats". Acta Scientific Medical Sciences 4.11 (2020): 89-96.


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